Human pancreatic triglyceride lipase expressed in yeast cells: Purification and characterization

Protein Expression and Purification

Volumn 13, Issue 1, 1998, Pages 36-40

  Yang, Yanqing ^a   Lowe, Mark E ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BLUE SEPHAROSE; CDNA CLONE; CHARACTERIZATION; CHROMATOGRAPHY; DIETHYLAMINOETHYLCELLULOSE; PURIFICATION; SIGNAL PEPTIDE; TRIGLYCERIDE LIPASE; YEAST CELL;

PICHIA PASTORIS;

EID: 0032103761     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0874     Document Type: Article

References (11)

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2. van Tilbeurgh H., Sarda L., Verger R., Cambillau C. Structure of the pancreatic lipase-procolipase complex. Nature. 359:1992;159-162.
3. van Tilbeurgh H., Egloff M. P., Martinez C., Rugani N., Verger R., Cambillau C. Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography. Nature. 362:1993;814-820.
4. Lowe M. E. The catalytic site residues and interfacial binding of human pancreatic lipase. J. Biol. Chem. 267:1992;17069-17073.
5. Jennens M. L., Lowe M. E. A surface loop covering the active site of human pancreatic lipase influences interfacial activation and lipid binding. J. Biol. Chem. 269:1994;25470-25474.
6. Cordle R. A., Lowe M. E. Purification and characterization of human procolipase expressed in yeast cells. Prot. Exp. Purif. 13:1997;30-35.
7. Lowe M. E. Mutation of the catalytic site Asp177 to Glu177 in human pancreatic lipase produces an active lipase with increased sensitivity to proteases. Biochim. Biophys. Acta. 1302:1996;177-183.
8. Lowe M. E. Human pancreatic procolipase expressed in insect cells: Purification and characterization. Prot. Exp. Purif. 5:1994;583-586.
9. Carriere F., Thirstrup K., Hjorth S., Ferrato F., Nielsen P. F., Withers-Martinez C., Cambillau C., Boel E., Thim L., Verger R. Pancreatic lipase structure-function relationships by domain exchange. Biochemistry. 36:1997;239-248.
10. Rogalska E., Cudrey C., Ferrato F., Verger R. Stereoselective hydrolysis of triglycerides by animal and microbial lipases. Chirality. 5:1993;24-30.
11. DeCaro A., Figarella C., Amic J., Michel R., Guy O. Human pancreatic lipase: A glycoprotein. Biochim. Biophys. Acta. 490:1977;411-419.