Elucidation of protein-protein interactions using chemical cross-linking or label transfer techniques

Current Opinion in Chemical Biology

Volumn 4, Issue 1, 2000, Pages 28-33

  Fancy, David A ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CROSS LINKING REAGENT; CYSTEINE; PORPHYRIN; PORPHYRIN DERIVATIVE; PROTEIN;

CATALYSIS; ESCHERICHIA COLI; METHODOLOGY; NONHUMAN; PHOTOAFFINITY LABELING; PROTEIN CROSS LINKING; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; REVIEW; STRUCTURE ANALYSIS;

ESCHERICHIA COLI;

EID: 0033970146     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(99)00047-2     Document Type: Review

References (19)

1. Li Y., Bjorklund S., Kim Y.J., Kornberg R.D. Yeast RNA polymerase II holoenzyme. Meth Enzymol. 273:1996;172-175.
2. Russell S.J., Reed S.H., Huang W., Friedberg E.C., Johnston S.A. The 19S regulatory complex of the proteasome functions independently of proteolysis in nucleotide excision repair. Mol Cell. 3:1999;687-695.
3. Knorre D.G., Godovikova T.S. Photoaffinity labeling as an approach to study supramolecular nucleoprotein complexes. FEBS Lett. 433:1998;9-14.
4. Marriott G., Ottl J. Synthesis and applications of heterobifunctional photocleavable cross-linking reagents. Meth Enzymol. 291:1998;155-175.
5. Chen Y., Ebright Y.W., Ebright R.H. Identification of the target of a transcription activator protein by protein-protein photocrosslinking. Science. 265:1994;90-92.
6. Ansari A.Z., Reece R.J., Ptashne M. A transcriptional activating region with two contrasting modes of protein interaction. Proc Natl Acad Sci USA. 95:1998;13543-13548.
7. Koh S.S., Ansari A.Z., Ptashne M., Young R.A. An activator target in the RNA polymerase II holoenzyme. Mol Cell. 1:1998;895-904. This study used photolabeling to indentify the Srb4-Gal4 AD protein interaction within a complex of 50 polypeptides. This demonstrates the power of the label-transfer technique.
8. Dey D., Bochkariov D.E., Jokhadze G.G., Traut R.R. Cross-linking of selected residues in the N- and C-terminal domains of Escherichia coli protein L7/L12 to other ribosomal proteins and the effect of elongation factor Tu. J Biol Chem. 273:1998;1670-1676.
9. 125I label transfer reagent. Bioconj Chem. 5:1994;205-212.
10. Brown K.C., Yang S.H., Kodadek T. Highly specific oxidative cross-linking of proteins mediated by a nickel-peptide complex. Biochemistry. 34:1995;4733-4739.
11. Bertrand R., Derancourt J., Kassab R. Probing the hydrophobic interactions in the skeletal actomyosin subfragment 1 and its nucleotide complexes by zero-length cross-linking with a nickel-peptide chelate. Biochem. 36:1997;9703-9714.
12. Brown K.C., Yu Z., Burlingame A.L., Craik C.S. Determining protein protein interactions by oxidative cross-linking of a glycine-glycine-histidine fusion protein. Biochemistry. 37:1998;4397. A chemical-genetic approach was used in this study, which showed the great chemical specificity possible through oxidative cross-linking.
13. Gill G., Richter R.A., Ghosh M., Burrows C.J., Rokita S.E. Nickel-dependent oxidative cross-linking of a protein. Chem Res Toxicol. 10:1997;302-309.
14. Fancy D.A., Melcher K., Johnston S.A., Kodadek T. New chemistry for the study of multiprotein complexes: the six-histidine tag as a receptor for a protein crosslinking reagent. Chem Biol. 3:1996;551-559.
15. Fancy D.A., Kodadek T. Site-directed oxidative protein crosslinking. Tetrahedron. 53:1997;11953-11960.
16. Campbell L.A., Kodadek T., Brown K.C. Protein cross-linking mediated by metalloporphyrins. Bioorg Med Chem. 6:1998;1301-1307.
17. Fancy D.A., Kodadek T. New chemistry for the analysis of protein protein interactions: rapid and efficient cross-linking triggered by long wavelength light. Proc Natl Acad Sci USA. 96:1999;6020-6024. A fast and efficient photochemical cross-linking approach is presented that should allow the study of cahnging protein-protein interactions in real time.
18. Kim K., Fancy D.A., Kodadek T. Photo-induced protein cross-linking mediated by palladium porphyrins. J Am Chem Soc. 1999;. in press.
19. Shriver D.F., Atkins P.W., Langford C.H. Inorganic chemistry. 1990;Oxford University Press, Oxford.