Constitutive expression of the cloned phenol hydroxylase gene(s) from Alcaligenes eutrophus JMP134 and concomitant trichloroethylene oxidation

Applied and Environmental Microbiology

Volumn 62, Issue 9, 1996, Pages 3227-3233

  Kim, Youngjun ^a,c   Ayoubi, Patricia ^a   Harker, Alan R ^b  

^a OKLAHOMA STATE UNIVERSITY   (United States)

^b BRIGHAM YOUNG UNIVERSITY   (United States)

^c The Catholic University of Korea   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CATECHOL 1,2 DIOXYGENASE; OXYGENASE; PHENOL; TOLUENE; TRICHLOROETHYLENE; UNSPECIFIC MONOOXYGENASE;

ARTICLE; BIODEGRADATION; COSMID; GENE EXPRESSION; NONHUMAN; OXIDATION; TRANSPOSON; WAUTERSIA EUTROPHA;

ALCALIGENES; BACTERIA (MICROORGANISMS); PSEUDOMONAS; RALSTONIA PICKETTII; WAUTERSIA EUTROPHA;

EID: 0029812685     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.9.3227-3233.1996     Document Type: Article

References (47)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Andersen, K., and M. Douglas. 1984. Construction and use of a gene bank of Alcaligenes eutrophus in the analysis of ribulose bisphosphate carboxylase genes. J. Bacteriol. 155:973-978.
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1989. Current protocols in molecular biology. John Wiley & Sons, Inc., New York.
4. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
5. Boyer, H. B., and D. Roulland-Dussoix. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:455-472.
6. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye binding. Anal. Biochem. 72:248-254.
7. Byrne, A. M., J. J. Kukor, and R. H. Olsen. 1995. Sequence analysis of the gene cluster encoding toluene-3-monooxygenase from Pseudomonas pickettii PKO1. Gene 154:65-70.
8. Dabrock, B., J. Riedel, J. Bertram, and G. Gottschalk. 1992. Isopropylbenzene (cumene) - a new substrate for the isolation of trichloroethene-degrading bacteria. Arch. Microbiol. 158:9-13.
9. Ditta, G., S. Stanfield, D. Corbin, and D. R. Helinski. 1980. Broad host range DNA cloning system for Gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77:7347-7351.
10. Don, R. H., and J. M. Pemberton. 1981. Properties of six pesticide degradation plasmids isolated from Alcaligenes paradoxus and Alcaligenes eutrophus. J. Bacteriol. 145:681-686.
11. Don, R. H., and J. M. Pemberton. 1985. Genetic and physical map of the 2,4-dichlorophenoxyacetic acid degradative plasmid pJP4. J. Bacteriol. 161: 466-468.
12. Don, R. H., A. J. Weightman, H.-J. Knackmuss, and K. N. Timmis. 1985. Transposon mutagenesis and cloning analysis of the pathways for degradation of 2,4-dichlorophenoxyacetic acid and 3-chlorobenzoate in Alcaligenes eutrophus JMP134(pJP4). J. Bacteriol. 161:85-90.
13. Folsom, B. R., P. J. Chapman, and P. H. Pritchard. 1990. Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates. Appl. Environ. Microbiol. 56:1279-1285.
14. Fürste, J. P., W. Pansegrau, R. Frank, H. Blöocker, P. Scholz, M. Bagdasarian, and E. Lanka. 1986. Molecular cloning of the RP4 DNA primase region in a multirange tacP expression vector. Gene 48:119-131.
15. Gish, W., and D. J. States. 1993. Identification of protein coding regions by database similarity search. Nat. Genet. 3:266-272.
16. Harker, A. R., and Y. Kim. 1990. Trichloroethylene degradation by two independent aromatic-degrading pathways in Alcaligenes eutrophus JMP134. Appl. Environ. Microbiol. 4:1179-1181.
17. Harker, A. R., R. H. Olsen, and R. J. Seidler. 1989. Phenoxyacetic acid degradation by the 2,4-dichlorophenoxyacetic acid pathway of the plasmid pJP4: mapping and characterization of the 2,4-D regulatory gene, tfdR. J. Bacteriol. 171:314-320.
18. Hegeman, G. D. 1966. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type. J. Bacteriol. 91:1140-1154.
19. Kaphammer, B., and R. H. Olsen. 1990. Cloning and characterization of tfdS, the repressor-activator gene of tfdB, from the 2,4-dichlorophenoxyacetic acid catabolic plasmid pJP4. J. Bacteriol. 172:5856-5858.
20. Knauf, V. C., and E. W. Nester. 1982. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid. Plasmid 8:45-54.
21. Kukor, J. J., and R. H. Olsen. 1990. Molecular cloning, characterization, and regulation of a Pseudomonas pickettii PKO1 gene encoding phenol hydroxylase and expression of the gene in Pseudomonas aeruginosa PAO1c. J. Bacteriol. 172:4584-4630.
22. Kukor, J. J., and R. H. Olsen. 1991. Genetic organization and regulation of a meta cleavage pathway for catechols produced from catabolism of toluene, benzene, phenol, and cresols by Pseudomonas pickettii PKO1. J. Bacteriol. 173:4587-4554.
23. Little, C. D., A. V. Palumbo, S. E. Herbes, M. E. Lidstrom, R. L. Tyndall, and P. J. Gilmer. 1988. Trichloroethylene biodegradation by a methane-oxidizing bacterium. Appl. Environ. Microbiol. 54:951-956.
24. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
25. Matrubutham, U., and A. R. Harker. 1994. Analysis of duplicated gene sequences associated with tfdR and tfdS in Alcaligenes eutrophus JMP134. J. Bacteriol. 176:2348-2353.
26. Nelson, M. J. K., S. O. Montgomery, W. R. Mahaffey, and P. H. Pritchard. 1987. Biodegradation of trichloroethylene and the involvement of an aromatic biodegradative pathway. Appl. Environ. Microbiol. 53:949-954.
27. Nelson, M. J. K., S. O. Montgomery, and P. H. Pritchard. 1988. Trichloroethylene metabolism by microorganisms that degrade aromatic compounds. Appl. Environ. Microbiol. 54:604-606.
28. Nozaki, M. 1970. Metapyrocatechase (Pseudomonas). Methods Enzymol. 17A:522-525.
29. Olsen, R. H., G. DeBusscher, and W. R. McCombie. 1982. Development of broad-host-range vectors and gene banks: self-cloning of the Pseudomonas aeruginosa PAO chromosome. J. Bacteriol. 150:60-69.
30. Perkins, E. J., M. P. Gordon, O. Caceres, and P. F. Lurquin. 1990. Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4. J. Bacteriol. 172:2351-2355.
31. Perkins, E. J., and P. F. Lurquin. 1988. Duplication of a 2,4-dichlorophenoxyacetic acid monooxygenase gene in Alcaligenes eutrophus JMP134 (pJP4). J. Bacteriol. 170:5669-5672.
32. Pieper, D. H., K. H. Engesser, and H. J. Knackmuss. 1989. Regulation of catabolic pathways of phenoxyacetic acids and phenols in Alcaligenes eutrophus JMP134. Arch. Microbiol. 151:356-371.
33. Pieper, D. H., W. Reineke, K. Engesser, and H. Knackmuss. 1988. Metabolism of 2,4-dichlorophenoxyacetic acid, 4-chloro-2-methylphenoxyacetic and 2-methylphenoxyacetic acid by Alcaligenes eutrophus JMP134. Arch. Microbiol. 150:95-102.
34. Rasche, M. E., M. R. Hyman, and D. J. Arp. 1991. Factors limiting aliphatic chlorocarbon degradation by Nitrosomonas europaea: cometabolic inactivation of ammonia monooxygenase and substrate specificity. Appl. Environ. Microbiol. 57:2986-2994.
35. Sala-Trepat, J. M., K. Murray, and P. A. Williams. 1972. The metabolic divergence in the meta-cleavage of catechols by Pseudomonas putida NCIB10015: physiological significance and evolutionary implications. Eur. J. Biochem. 28:347-356.
36. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
37. Shields, M. S., S. O. Montgomery, S. M. Cuskey, P. J. Chapman, and P. H. Prilchard. 1991. Mutants of Pseudomonas cepacia C4 defective in catabolism of aromatic compounds and trichloroethylene. Appl. Environ. Microbiol. 7:1935-1941.
38. Shields, M. S., and M. J. Reagin. 1992. Selection of a Pseudomonas cepacia strain constitutive for the degradation of trichloroethylene. Appl. Environ. Microbiol. 58:3977-3983.
39. Simon, R., U. Priefer, and A. Puhler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1:748-791.
40. Spain, J. C., G. J. Zylstra, C. K. Blake, and D. T. Gibson. 1989. Monohydroxylation of phenol and 2,5-dichlorophenol by toluene dioxygenase in Pseudomonas putida F1. Appl. Environ. Microbiol. 55:2648-2652.
41. Stanier, R., N. Palleroni, and M. Doudoroff. 1966. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43:159-271.
42. Streber, W., K. N. Timmis, and M. H. Zenk. 1987. Analysis, cloning, and high-level expression of 2,4-dichlorophenoxy-acetate monooxygenase gene tfdA of Alcaligenes eutrophus JMP134. J. Bacteriol. 169:2950-2955.
43. Tsein, H. C., G. A. Brussean, R. S. Hanson, and L. P. Wackett. 1989. Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b. Appl. Environ. Microbiol. 55:3155-3161.
44. Wackett, L. P., G. A. Brusseau, S. R. Householder, and R. S. Hanson. 1989. A survey of microbial oxygenases: trichloroethylene degradation by propane-oxidizing bacteria. Appl. Environ. Microbiol. 55:2960-2964.
45. Wackett, L. P., and S. R. Householder. 1989. Toxicity of trichloroethylene to Pseudomonas putida F1 is mediated by toluene dioxygenase. Appl. Environ. Microbiol. 55:2723-2725.
46. Weiss, A. A., E. L. Hewlett, G. A. Myers, and S. Falkow. 1983. Tn5-induced mutations affecting virulence factors of Bordetella pertussis. Infect. Immun. 42:33-41.
47. Winter, R. B., K. M. Yen, and B. D. Ensley. 1989. Efficient degradation of trichloroethylene by a recombinant Escherichia coli. Bio/Technology 7:282-285.