Intracellular protein degradation and autophagy in isolated pancreatic acini of the rat

Cell Biochemistry and Function

Volumn 18, Issue 1, 2000, Pages 29-40

  Telbisz, Agnes ^a   Kovacs, Attila L ^a  

^a EÖTVÖS LORÁND UNIVERSITY   (Hungary)

Author keywords

Amino acids; Inhibition; Lysosomal protein breakdown

Indexed keywords

3 METHYLADENINE; AMINE; CELL PROTEIN; LEUPEPTIN; VINBLASTINE;

ACINAR CELL; ANIMAL CELL; ARTICLE; AUTOPHAGY; BIOCHEMISTRY; CELL STRUCTURE; CELL VACUOLE; LIVER CELL; LYSOSOME; MALE; MEMBRANE FUSION; MORPHOMETRICS; NONHUMAN; PHAGOSOME; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; RAT; STEADY STATE;

ADENINE; AMINES; AMINO ACIDS; ANIMALS; AUTOPHAGY; CELLS, CULTURED; ENZYME INHIBITORS; KINETICS; LEUPEPTINS; LYSOSOMES; MALE; MICROSCOPY, ELECTRON; PANCREAS; PROTEIN BIOSYNTHESIS; PROTEINS; RATS; RATS, LONG-EVANS; VINBLASTINE;

EID: 0033965924     PISSN: 02636484     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-0844(200001/03)18:1<29::AID-CBF844>3.0.CO;2-G     Document Type: Article

References (56)

1. Ballard, F. J. (1987). Regulation of intracellular protein breakdown with special reference to cultured cells. In: Lysosomes: Their Role in Protein Breakdown. (Glaumann, H. and Ballard, F. J., eds). Academic Press Inc. Ltd: London, pp. 285-314.
2. Gordon, P. B., Kisen, G. O., Kovacs, A. L. and Seglen, P. O. (1989). Experimental characterization of the autophagiclysosomal pathway in isolated rat hepatocytes. Biochem. Soc. Symp., 55, 129-143.
3. Hendil, K. B., Lauridsen, A. M. and Seglen, P. O. (1990). Both endocytic and endogenous protein degradation in fibroblasts is stimulated by serum/amino acid deprivation and inhibited by 3-methyladenine. Biochem. J., 272, 577-581.
4. Hopgood, M. F., Clark, M. G. and Ballard, F. J. (1977). Inhibition of protein degradation in isolated rat hepatocyles. Biochem. J., 164, 399-407.
5. Kovacs, A. L., Grinde, B. and Seglen, P. O. (1981). Inhibition of autophagic vacuole formation and protein degradation by amino acids in isolated hepatocytes. Exp. Cell Res., 133, 431-436.
6. Mortimore, G. E. and Poso, A. R. (1984). Lysosomal pathways in hepatic protein degradation: regulatory role of amino acids. Fed. Proc., 43, 1289-1294.
7. Rabkin, R., Tsao, T., Shi, J. D. and Mortimore, G. (1991). Amino acids regulate kidney cell protein breakdown. J. Lab. Clin. Med., 117, 505-513.
8. Scornik, O. A. (1984). Effects of inhibitors of protein degradation on the rate of protein synthesis in Chinese hamster ovary cells. J. Cell. Physiol., 121, 257-262.
9. Seglen, P. O. and Gordon, P. B. (1984). Amino acid control of autophagic sequestration and protein degradation in isolated rat hepatocytes. J. Cell Biol., 99, 435-444.
10. Warburton, M. J. and Poole, B. (1977). Effect of medium composition on protein degradation and DNA synthesis in rat embryo libroblasts. Proc. Natl. Acad. Sci. USA, 74, 2427-2431.
11. Mortimore, G. E. (1987). Mechanism and regulation of induced and basal protein degradation in liver. In: Lysosomes: Their Role in Protein Breakdown. (Glaumann, H. and Ballard, F. J., eds). Academic Press Inc. Ltd: London, pp. 415-441.
12. Mortimore, G. E. and Schworer, C. M. (1977). Induction of autophagy by amino acid deprivation in perfused liver. Nature, 270, 174-176.
13. Seglen, P. O. (1987). Regulation of autophagic protein degradation in isolated liver cells. In: Lysosomes: Their Role in Protein Breakdown. (Glaumann, H. and Ballard, F. J., eds). Academic Press Inc. Ltd: London, pp.371-410.
14. Seglen, P. O. and Bohley, P. (1992). Autophagy and other vacuolar protein degradation mechanisms. Experientia, 48, 158-171.
15. Kovacs, A. L., Laszlo, L., Fellinger, E., Jakab, A., Orosz, A., Rez, G. and Kovacs, J. (1989). Combined effects of lasting and vinblastine treatment on serum insulin level, the size of autophagic-lysosomal compartment, protein content and lysosomal enzyme activities of liver and exocrine pancreatic cells of the mouse. Comp. Biochem. Physiol., B94, 505-510.
16. Kovacs, A. L., Laszlo, L., Tajti, L. and Kovacs, J. (1981). Dose dependence of vinblastine-induced autophagocytosis in liver parenchymal, exocrine pancreas and seminal vesicle epithelial cells of the mouse in vivo. Acta Biol. Acad. Sci. Hung., 32, 157-159.
17. Kovacs, J., Laszlo, L. and Kovacs, A. L. (1988). Regression of autophagic vacuoles in pancreatic acinar, seminal vesicle epithelial, and liver parenchymal cells: a comparative morphometric study of the effect of vinblastine and leupeptin followed by cycloheximide treatment. Exp. Cell Res., 174, 244-251.
18. Rez, G., Csak. J., Fellinger, E., Laszlo, L., Kovacs, A. L., Oliva, O. and Kovacs, J. (1996). Time course of vinblastine-induced autophagocytosis and changes in the endoplasmic reticulum in murine pancreatic acinar cells: a morphometric and biochemical study. Eur. J. Cell Biol., 71, 341-350.
19. Willemer, S. and Adler, G. (1991). Mechanism of acute pancreatitis. Cellular and subcellular events. Int. J. Pancreatol., 9, 21-30.
20. Tooze, J., Hollinshead, M., Ludwig, T., Howell, K., Hoflack, B. and Kern, H. (1990). In exocrine pancreas, the basolateral endocytic pathway converges with autophagic pathway immediately after the early endosome. J. Cell Biol., 111, 329-345.
21. Telbisz, A. and Kovacs, A. L. (1999). Complete dissociation of rat pancreas into acini by a perfusion-incubation method. Measurement of protein synthesis and the degradation of endogenous proteins. Pancreas, 18(4), 342-348.
22. Kovacs, J. and Rez, G. (1989). Quantitative aspects of cellular autophagocytosis. Morphometric and cell fractionation studies. Revis. Biol. Celular, 20, 63-78.
23. Marzella, L. and Glaumann, H. (1987). Autophagy, microautophagy and crinophagy as mechanisms for protein degradation. In: Lysosomes: Their Role in Protein Breakdawn. (Glaumann, H. and Ballard, F. J., eds). Academic Press Inc. Ltd: London, pp. 319-362.
24. Pfeifer, U. (1987). Functional morphology of the lysosomal apparatus. In: Lysosomes: Their Role in Protein Breakdown. (Glaumann, H. and Ballard, F. J., eds). Academic Press Inc. Ltd: London, pp. 3-48.
25. Kovacs, A. L., Reith, A. and Seglen, P. O. (1982). Accumulation of autophagosomes after inhibition of hepatocytic protein degradation by vinblastine, leupeptin or a lysosomotropic amine. Exp. Cell Res., 137, 191-201.
26. Seglen, P. O., Grinde, B. and Solheim, A. E. (1979). Inhibition of the lysosomal pathway of protein degradation in isolated rat hepatocytes by ammonia, methylamine, chloroquine and leupeptin. Eur. J. Biochem., 95, 215-225.
27. Seglen, P. O. and Gordon, P. B. (1982). 3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc. Natl. Acad. Sci. USA. 79, 1889-1892.
28. Neely, A. N., Cox, J. R., Fortney, J. A., Schworer, C. and Mortimore, G. E. (1977). Alterations of lysosomal size and density during rat liver perfusion. Suppression by insulin and amino acids. J. Biol. Chem., 252, 6948-6954.
29. Scornik, A. and Botbol, V. (1987). Protein metabolism and liver growth. In: Lysosomes: Their Role in Protein Breakdown. (Glaumann, H. and Ballard, F. J . eds). Academic Press Inc. Ltd: London, pp. 445-486.
30. Gorham, L. W. and Waymouth, C (1965). Differentiation in vitro of embryonic cartilage and hone in a chemically defined medium. Proc. Soc. Exp. Biol. Med., 119, 287-290.
31. Seglen, P. O. (1976). Preparation of isolated rat liver cells. In: Methods in Cell Biology. Vol. 13. (Prescott, D. M., ed.). Academic Press: New York, pp. 29-83.
32. Mortimore, G. E. and Mondon, C. E. (1970). Inhibition by insulin of valine turnover in liver. Evidence for a general control of proteolysis. J. Biol. Chem., 245, 2375-2383.
33. Seglen, P. O. and Solheim, A. E. (1978). Valine uptake and incorporation into protein in isolated rat hepatocytes, nature of the precursor pool for protein synthesis. Eur. J. Biochem., 85, 15-25.
34. Weibel, E. R. (1969). Stereological principles for morphometry in electronmicroscopic cytology. Int. Rev. Cytol., 26, 235-302.
35. Williams, M. A. (1977). Quantitative Methods in Biology; Part II. Chapter 2.6, 2.7, North-Holland: Amsterdam New York-Oxford.
36. Seglen, P. O. (1978). Effects of amino acids, ammonia and leupeptin on protein synthesis and degradation in isolated rat hepatocytes. Biochem, J., 174, 469-474.
37. Seglen, P. O., Gordon, P. B., Grinde, B. Solheim, A., Kovacs, A. L. and Poli, A. (1981). Inhibitors and pathways of hepatocytic protein degradation. Acta Biol. Med. Germ., 40, 1587-1598.
38. Schworer, C. M., Shiffer, K. A. and Mortimore, G. E. (1981). Quantitative relationship between autophagy and proteolysis during graded amino acid deprivation in perfused rat liver. J. Biol. Chem., 256, 7652-7658.
39. Wheatley, D. N., Grisolia, S. and Hernandez-Yago, J. (1982). Significance of the rapid degradation of newly synthesized proteins in mammalian cells: a working hypothesis. J. Theoret. Biol., 98, 283-300.
40. Kovacs, A. L., Laszlo, L. and Kovacs, J. (1985). Effect of amino acids and cycloheximide on changes caused by vinblastine, leupeptin and methylamine in the autophagic/ lysosomal system of mouse hepatocytes in vivo. Exp. Cell. Res., 157, 83-94.
41. Kovacs, J., Rez, G., Kovacs, A. L., Csak, J. and Zboray, G. (1982). Autophagocytosis: freeze-fracture morphology, effects of vinblastine and influence of transcriptional and translational inhibitors. Acta Biol. Med. Germ., 41, 131-135.
42. Kovacs, A. L. and Seglen, P. O. (1982). Inhibition of hepatocytic protein degradation by inducers of autophagosome accumulation. Acta Biol. Med. Germ., 41, 125-130.
43. Fengsrud, M., Roos, N., Berg, T., Liou, W. L., Slot, J. W. and Seglen, P. O. (1995). Ultrastructural and immunocytochemical characterization of autophagic vacuoles in isolated hepatocytes: Effects of vinblastine and asparagine on vacuole distributions. Exp. Cell Res., 221, 504-519.
44. Gordon, P. B., Hoyvik, H. and Seglen, P. O. (1992). Prelysosomal and lysosomal connections between autophagy and endocytosis. Biochem. J., 283, 361-369.
45. Rez, G., Fellinger, E., Oliva, O., Palfia, Z., Csak, J., Laszlo, L., Kovacs, A. L., Kovacs, J. and Karpati, A. P. (1991). Mechanism and dynamics of macroautophagy in murine exocrine pancreatic cells. A review of vinblastine-induced changes. Acta Biol. Hung., 42, 57-86.
46. Caro, L. H., Plomp, P J., Wolvetang, E. J., Kerkhof, C. and Meijer, A. J. (1988). 3-Methyladenine, an inhibitor of autophagy, has multiple effects on metabolism. Eur. J. Bichem., 175, 325-329.
47. Chandler, C. S. and Ballard, F. J. (1988). Regulation of the breakdown rates of biotin-containing proteins in Swiss 3T3-L1 cells. Biochem. J., 251, 749-755.
48. Houri, J. J., Ogier-Denis, E., Trugnan, G. and Codogno, P. (1993). Autophagic degradation of N-linked glycoproteins is downregulated in differentiated human colon adenocarcinoma cells. Biochem. Biophys. Res. Commun., 197, 805-811.
49. Jia, L., Dourmashkin, R. R., Allen, P. D., Gray, A. B., Newland, A. C. and Kelsey, S. M. (1997). Inhibition of autophagy abrogates tumour necrosis factor alpha induced apoptosis in human T-lymphoblastic leukaemic cells. Br. J. Haematol., 98, 673-685.
50. Punnonen, E. L. and Reunanen, H. (1990). Effects of vinblastine. leucine, and histidine, and 3-methyladenine on autophagy in Ehrlich ascites cells. Exp. Mol. Pathol., 52, 87-97.
51. Schwarze, P. E., Solheim, A. E. and Seglen, P. O. (1982). Amino acid and energy requirements for rat hepatocytes in primary culture. In vitro, 18, 43-54.
52. Kovacs, A. L. and Seglen, P. O. (1981). Inhibition of hepatocytic protein degradation by methylaminopurines and inhibitors of protein synthesis. Biochim. Biophys. Acta, 676, 213-220
53. De Lisle, R. C. and Williams, J. A. (1987). Zymogen granule acidity is not required for stimulated pancreatic protein secretion. Am. J. Phaysiol., 253, G711-G719.
54. Frexes-Steed, M., Warner, M. L., Bulus, N., Flakoll, P. and Abumrad, N. N. (1990). Role of insulin and branched-chain amino acids in regulating protein metabolism during fasting. Am. J. Physiol., 258, E907-E917.
55. Mann, G. E. and Peran, S. (1986). Basolateral amino acid transport system in the perfused exocrine pancreas:sodium-dependency and kinetic interactions between influx and efflux mechanisms. Biochim. Biophys. Acta. 585, 263-274.
56. Van Venrooij, W. J., Poort, C., Kramer, M. F. and Jansen, M. T. (1972). Relationship between extracellular amino acids and protein synthesis in vitro in the rat pancreas. Eur. J. Biochem., 30, 427-433.