Complete dissociation of rat pancreas into acini by a perfusion- incubation method: Measurement of protein synthesis and the degradation of endogenous proteins

Pancreas

Volumn 18, Issue 4, 1999, Pages 342-348

  Telbisz, Agnes ^a   Kovacs, Attila L ^a  

^a EÖTVÖS LORÁND UNIVERSITY   (Hungary)

Author keywords

Exocrine cells; Insulin; Isolation technique; Ultrastructure; Uniform suspension

Indexed keywords

LEUPEPTIN;

ANIMAL TISSUE; ARTICLE; BASEMENT MEMBRANE; CELL POLARITY; CELL VIABILITY; CONTROLLED STUDY; DESMOSOME; ELECTRON MICROSCOPY; NONHUMAN; PANCREAS ACINUS; PANCREAS PERFUSION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; RAT; TIGHT JUNCTION;

EID: 0032960435     PISSN: 08853177     EISSN: None     Source Type: Journal    
DOI: 10.1097/00006676-199905000-00003     Document Type: Article

References (17)

1. Williams JA, Korc M, Dormer RL. Action of secretagogue on a new preparation of functionally intact, isolated pancreatic acini. Am J Physiol 1978;235:E517-24.
2. Amsterdam A, Jamieson JD. Studies on dispersed pancreatic exocrine cells I: dissociation technique and morphologic characteristics of separated cells. J Cell Biol 1974;63:1037-56.
3. Arias AE, Bendayan M. Secretagogue induction of cell differentiation in pancreatic acinar cells in vitro. Exp Cell Res 1991;195: 199-205.
4. Williams JA, Gary P, Moffat B. Effects of ions on amylase release by dissociated pancreatic acinar cells. Am J Physiol 1976;231: 1562-7.
5. Bendayan M, Duhr M, Gingras D. Studies on pancreatic cells in tissue culture: basal lamina (basement membrane) matrix promotes three-dimensional reorganisation. Eur J Cell Biol 1986;42:60-7.
6. Livne E, Oliver C. Internalisation of cationised ferritin by isolated pancreatic acinar cells. J Histochem Cytochem 1986;34:167-76.
7. Seglen PO. Preparation of isolated rat liver cells. Methods Cell Biol 1976;13:29-83.
8. Gorham LW, Waymouth C. Differentiation in vitro of embryonic cartilage and bone in a chemically defined medium. Proc Soc Exp Biol Med 1965;119:287-90.
9. Kovacs AL, Laszlo L, Fellinger E, et al. Combined effects of fasting and vinblastine treatment on serum insulin level, the size of autophagic-lysosomal compartment, protein content and lysosomal enzyme activities of liver and exocrine pancreatic cells of the mouse. Comp Biochem Physiol B 1989;94:505-10.
10. Hebel R, Stromberg MV. Anatomy and embryology of the laboratory rat. Germany: Biomed Verlag Wörthsee, 1986.
11. Letko G, Spormann H, Sokolowski A, Schulz HU. Pancreatic acinar cells: isolation, characterisation and application in physiologic and pathophysiologic studies, with special reference to acute pancreatitis. Exp Pathol 1988;34:10-23.
12. Lopes JM, Larbier M. Influence of amino-acid concentration in the culture medium on the rate of amino-acid incorporation into protein and amino-acid oxidation of cultured chicken hepatocytes. Reprod Nutr Dev 1994;34:157-64.
13. Clark RM, Chandler PT, Park CS, Norman AW. Extracellular amino acid effects on milk protein synthesis and intracellular amino acid pools with bovine mammary cells in culture. J Dairy Sci 1980;8:1230-4.
14. Willemer S, Adler G. Mechanism of acute pancreatitis: cellular and subcellular events. Int J Pancreatol 1991;9:21-30.
15. Amsterdam A, Jamieson JD. Studies on dispersed pancreatic exocrine cells II: functional characteristics of separated cells. J Cell Biol 1974;63:1057-73.
16. Seglen PO, Gordon PB, Grinde B, Solheim A, Kovacs AL, Poli A. Inhibitors and pathways of hepatocytic protein degradation. Acta Biol Med Ger 1981;40:1587-98.
17. Seglen PO. Effects of amino acids, ammonia and leupeptin on protein synthesis and degradation in isolated rat hepatocytes. Biochem J 1978;174:469-74.