Imaging microtubules and kinesin decorated microtubules using tapping mode atomic force microscopy in fluids

European Biophysics Journal

Volumn 28, Issue 8, 2000, Pages 611-620

  Kacher, Claudia M ^a   Weiss, Ingrid M ^a   Stewart, Russell J ^b   Schmidt, Christoph F ^c   Hansma, Paul K ^d   Radmacher, Manfred ^e   Fritz, Monika ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b UNIVERSITY OF UTAH   (United States)

^c VU UNIVERSITY AMSTERDAM   (Netherlands)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e UNIVERSITY OF MUNICH   (Germany)

Author keywords

Atomic force microscopy; Kinesin; Microtubules

Indexed keywords

ADENOSINE TRIPHOSPHATE DERIVATIVE; ADENYLYLIMIDODIPHOSPHATE; GLASS; KINESIN; PALMITIC ACID DERIVATIVE; SILANE DERIVATIVE;

AQUEOUS SOLUTION; ARTICLE; ATOMIC FORCE MICROSCOPY; LIPID BILAYER; MICROTUBULE; OPTICAL RESOLUTION; SOLID; STRUCTURE ANALYSIS;

EID: 0033964571     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002490050001     Document Type: Article

References (41)

1. Bezanilla M, Drake B, Nudler E, Kashlev M, Hansma PK, Hansma HG (1994) Motion and enzymatic degradation of DNA in the atomic force microscope. Biophys J 67: 1-6
2. Bezanilla M, Manne S, Laney DE, Lyubchenko YL, Hansma HG (1995) Adsorption of DNA to mica, silylated mica and minerals: characterization by atomic force microscopy. Langmuir 11: 655-659
3. Binnig G, Quate CF, Gerber C (1986) Atomic force microscope. Phys Rev Lett 56: 930
4. Block SM (1997) Real engines of creation. Nature 386: 217-219
5. Bloom GS, Endow SA (1994) Motor proteins 1: kinesins. Protein Profile 1: 1059-1106
6. Bloom GS, Wagner MC, Pfister KK, Brady ST (1988) Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide. Biochemistry 27: 3409-3416
7. Brinkley WBR (1997) Microtubules: a brief historical perspective. J Struct Biol 118: 84-86
8. Fritz M, Radmacher M, Gaub HE (1994) Granula motion and membrane spreading during activation of human platelets imaged by atomic force microscopy. Biophys J 66: 1328-1334
9. Fritz M, Radmacher M, Allersma M, Cleveland J, Stewart R, Hansma P, Schmidt C (1995a) Imaging microtubules in buffer solution using tapping mode atomic force microscopy. Proc SPIE 2384: 150-157
10. Fritz M, Radmacher M, Cleveland JP, Gieselmann R, Allersma MW, Stewart RJ, Schmidt CF, Hansma PK (1995b) Imaging globular and filamentous proteins in physiological buffer solution in tapping mode atomic force microscopy. Langmuir 11: 3529-3535
11. Hansma PK, Cleveland JP, Radmacher M, Walters DA, Hillner PE, Bezanilla M, Fritz M, Vie D, Hansma HG, Prater CB, Massie J, Fukunaga L, Gurley J, Elings V (1994) Tapping mode atomic force microscopy in liquids. Appl Phys Lett 64: 1738-1740
12. Hirokawa N (1998) Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science 279: 519-5265
13. Hirose K, Lockhart A, Cross RA, Amos LA (1995) Nucleotide-dependent angular change in kinesin motor domain bound to tubulin. Nature 376: 277-279
14. Hoenger A, Sack S, Thormählen M, Marx A, Müller J, Gross H, Mandelkow E (1998) Image reconstructions of microtubules decorated with monomeric and dimeric kinesins: comparison with X-ray structure and implications for motility. J Cell Biol 141: 419-430
15. Hoh JH, Schoenenberger C-A (1994) Surface morphology and mechanical properties of MDCK monolayers by atomic force microscopy. Biophys J 107: 1105-1114
16. Hoh J, Sosinsky GE, Revel J-P, Hansma PK (1993) Structure of the extracellular surface of the gap junction by atomic force microscopy. Biophys J 65: 149-163
17. Hyams JS, Lloyd CW (1994) Microtubules. Wiley-Liss, New York
18. Karrasch S, Dolder M, Schabert F, Ramsden J, Engel A (1993) Covalent binding of biological samples to solid supports for scanning probe microscopy in buffer solution. Biophys J 65: 2437-2446
19. Karrasch S, Hegerl R, Hoh JH, Baumeister W, Engel A (1994) Atomic force microscopy produces faithful high-resolution images of protein surfaces in an aqueous environment. Proc Natl Acad Sci USA 91: 836-838
20. Keller D, Deputy D, Alduino A, Luo K (1992) Sharp, vertical-walled tips for SFM imaging of steep or soft samples. Ultramicroscopy 42-44: 1481-1489
21. Kikkawa M, Ishikawa T, Wakabayashi T, Hirokawa N (1995) Three-dimensional structure of the kinesin head-microtubule complex. Nature 376: 274-276
22. Mandelkow E-M, Schultheiss R, Rapp R, Müller M, Mandelkow E (1986) On the surface lattice of microtubules: helix starts, protofilament number, seam, and handedness. J Cell Biol 102: 1067-1073
23. Metoz F, Wade RH (1997) Diffraction by helical structures with seams: microtubules. J Struct Biol 118: 128-139
24. Müller DJ, Büldt G, Engel A (1995a) Force-induced conformational change of bacteriohodopsin. J Mol Biol 249: 239-243
25. Müller DJ, Schabert FA, Büldt G, Engel A (1995b) Imaging purple membranes in aqueous solutions at sub-nanometer resolution by atomic force microscopy. Biophys J 68: 1681-1686
26. Nogales E, Wolf SG, Khan I, Luduena RF, Downig KH (1995) Structure of tubulin at 6.5 Å and location of the taxol-binding site. Nature 375: 424-427
27. Nogales E, Wolf SG, Downing KH (1997) Visualizing the secondary structure of tubulin: three-dimensional map at 4 Å. J Struct Biol 118: 119-127
28. Radmacher M, Tillmann RW, Fritz M, Gaub HE (1992) From molecules to cells - imaging soft samples with the AFM. Science 257: 1900-1905
29. Radmacher M, Fritz M, Hansma HG, Hansma PK (1994) Direct observation of enzyme activity with the atomic force microscope. Science 265: 1577-1579
30. Radmacher M, Fritz M, Kacher CM, Cleveland JP, Hansma PK (1996) Measuring viscoelastic properties of human platelets with the atomic force microscope. Biophys J 70: 556-567
31. Schabert FA, Rabe JP (1996) Vertical dimension of hydrated biological samples in tapping mode scanning force microscopy. Biophys J 70: 1514-1520
32. Svoboda K, Schmidt CF, Schnapp BJ, Block SM (1993) Direct observation of kinesin stepping by optical trapping interferometry. Nature 365: 721-727
33. Thormählen M, Marx A, Müller SA, Song Y-H, Mandelkow E-M, Aebi U, Mandelkow E (1998) Interaction of monomeric and dimeric kinesin with microtubules. J Cell Biol 141: 419-430
34. Vale RD, Reese TS, Sheetz MP (1985) Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Cell 42: 39-50
35. Vinckier A, Heyvaert I, D'Hoore A, McKittrick T, Van Haesendonck C, Engelborghs Y, Hellemans L (1995) Immobilizing and imaging microtubules by atomic force microscopy. Ultramicroscopy 57: 337-343
36. Wade RH, Hyman AA (1997) Microtubule structure and dynamics. Curr Opin Cell Biol 9: 12-17
37. Wendel M, Lorenz H, Kotthaus JP (1995) Sharpened electron beam deposited tips for high resolution atomic force microscope lithography and imaging. Appl Phys Lett 67: 3732-3734
38. Williams RCJ, Lee JC (1982) Preparation of tubulin from brain. Methods Enzymol 85: 376-385
39. Yang JT, Saxton WM, Stewart RJ, Raff E, Goldstein LSB (1990) Evidence that the head of kinesin is sufficient for force generation and motility in vitro. Science 249: 42-47
40. Yang J, Tamm LK, Tillack TW, Shao Z (1993) New approach for atomic force microscopy of membrane proteins - the imaging of cholera toxin. J Mol Biol 229: 286-290
41. Zantl R, Artzner F, Rapp G, Rädler JO (1999) Thermotropic structural changes of saturated-cationic-lipid-DNA complexes. Europhys Lett 45: 90-96