Clustered charge-to-alanine mutagenesis of the vaccinia virus H5 gene: Isolation of a dominant, temperature-sensitive mutant with a profound defect in morphogenesis

Journal of Virology

Volumn 74, Issue 5, 2000, Pages 2393-2405

  Demasi, Joseph ^b   Traktman, Paula ^a  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; VIRUS PROTEIN;

ANIMAL CELL; ARTICLE; GENE EXPRESSION; GENE REPLICATION; HUMAN; HUMAN CELL; MUTAGENESIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; TEMPERATURE SENSITIVE MUTANT; VACCINIA VIRUS; VIRUS GENE; VIRUS ISOLATION; VIRUS MORPHOGENESIS; VIRUS MUTANT;

ALANINE; ALLELES; AMINO ACID SEQUENCE; ANIMALS; GENES, DOMINANT; GENES, VIRAL; L CELLS (CELL LINE); MICE; MICROSCOPY, ELECTRON; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLAQUE ASSAY; TEMPERATURE; VACCINIA VIRUS; VIRAL PROTEINS; VIRION; VIRUS REPLICATION;

EID: 0033962255     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.5.2393-2405.2000     Document Type: Article

References (54)

1. Banham, A. H., D. P. Leader, and G. L. Smith. 1993. Phosphorylation of ribosomal proteins by the vaccinia virus B1R protein kinase. FEBS Lett. 321:27-31.
2. Banham, A. H., and G. L. Smith. 1992. Vaccinia virus gene B1R encodes a 34-kDa serine/threonine protein kinase that localizes in cytoplasmic factories and is packaged into virions. Virology 191:803-812.
3. Beaud, G., and R. Beaud. 1997. Preferential virosomal location of underphosphorylated H5R protein synthesized in vaccinia virus-infected cells. J. Gen. Virol. 78:3297-3302.
4. Beaud, G., R. Beaud, and D. P. Leader. 1995. Vaccinia virus gene H5R encodes a protein that is phosphorylated by the multisubstrate vaccinia virus B1R protein kinase. J. Virol. 69:1819-1826.
5. Beaud, G., A. Sharif, A. Topa-Masse, and D. P. Leader. 1994. Ribosomal protein S2/Sa kinase purified from HeLa cells infected with vaccinia virus corresponds to the B1R protein kinase and phosphorylates in vitro the viral ssDNA-binding protein. J. Gen. Virol. 75:283-293.
6. Betakova, T., E. J. Wolffe, and B. Moss. 1999. Regulation of vaccinia virus morphogenesis: phosphorylation of the A14L and A17L membrane proteins and C-terminal truncation of the A17L protein are dependent on the F10L kinase. J. Virol. 3:3534-3543.
7. Black, E. P., N. Moussatche, and R. C. Condil. 1998. Characterization of the interactions among vaccinia virus transcription factors G2R, A18R, and H5R. Virology 245:313-322.
8. Black, P., and R. C. Condil. 1996. Phenotypic characterization of mutants in vaccinia virus gene G2R, a putative transcription elongation factor. J. Virol. 70:47-54.
9. Chakrabarti, S., J. R. Sisler, and B. Moss. 1997. Compact, synthetic, vaccinia virus early/late promoter for protein expression. BioTechniques 23:1094-1097.
10. Condit, R. C., A. Motyczka, and G. Spizz. 1983. Isolation, characterization and physical mapping of temperature sensitive mutants of vaccinia virus. Virology 128:429-443.
11. Covi, J. A., J. M. Belote, and D. L. Mykles. 1999. Subunit compositions and catalytic properties of proteasomes from developmental temperature-sensitive mutants of Drosophila melanogaster. Arch. Biochem. Biophys. 368:85-97.
12. Derrien, M., A. Punjabi, M. Khanna, O. Grubisha, and P. Traktman. 1999. Tyrosine phosphorylation of A17 during vaccinia virus infection: involvement of the H1 phosphatase and the F10 kinase. J. Virol. 73:7287-7296.
13. Diamond, S. E., and K. Kirkegaard. 1994. Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis. J. Virol. 68:863-876.
14. Ensinger, M., and M. Rovinsky. 1983. Marker rescue of temperature sensitive mutations of vaccinia virus WR: correlation of genetic and physical maps. J. Virol. 48:419-428.
15. Evans, E., and P. Traktman. 1987. Molecular genetic analysis of a vaccinia virus gene with an essential role in DNA replication. J. Virol. 61:3152-3162.
16. Falkner, F. G., and B. Moss. 1990. Transient dominant selection of recombinant vaccinia viruses. J. Virol. 64:3108-3111.
17. Gibbs, C. S., and M. J. Zoller. 1991. Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions. J. Biol. Chem. 266:8923-8931.
18. Goebel, S. J., G. P. Johnson, M. E. Perkus, S. W. David, J. P. Winslow, and E. Paoletti. 1990. The complete DNA sequence of vaccinia virus. Virology 179:247-266.
19. Gordon, J., A. Mohandas, S. Wilton, and S. Dales. 1991. A prominent antigenic surface polypeptide involved in the biogenesis and function of the vaccinia virus envelope. Virology 181:671-686.
20. Grimley, P. M., E. N. Rosenblum, S. J. Mims, and B. Moss. 1970. Interruption by rifampicin of an early stage in vaccinia virus morphogenesis: accumulation of membranes which are precursors of virus envelopes. J. Virol. 6:519-533.
21. Guan, K., S. S. Broyles, and J. E. Dixon. 1991. A tyr/ser phosphatase encoded by vaccinia virus. Nature 350:359-362.
22. Hassett, D. E., and R. C. Condit. 1994. Targeted construction of temperature-sensitive mutations in vaccinia virus by replacing clustered charged residues with alanine. Proc. Natl. Acad. Sci USA 91:4554-4558.
23. Hassett, D. E., J. I. Lewis, X. Xing, L. DeLange, and R. C. Condit. 1997. Analysis of a temperature-sensitive vaccinia virus mutant in the viral mRNA capping enzyme isolated by clustered charge-to-alanine mutagenesis and transient dominant selection. Virology 238:391-409.
24. Kane, E. M., and S. Shuman. 1993. Vaccinia virus morphogenesis is blocked by a temperature-sensitive mutation in the 17 gene that encodes a virion component. J. Virol. 67:2689-2698.
25. Klemperer, N., J. Ward, E. Evans, and P. Traktman. 1997. The vaccinia virus I1 protein is essential for the assembly of mature virions. J. Virol. 71:9285-9294.
26. Kovacs, G. R., and B. Moss. 1996. The vaccinia virus H5R gene encodes late gene transcription factor 4: purification, cloning, and overexpression. J. Virol. 70:6796-6802.
27. Lin, S., and S. S. Broyles. 1994. Vaccinia protein kinase 2: a second essential serine/threonine kinase encoded by vaccinia virus. Proc. Natl. Acad. Sci. USA 91:7653-7657.
28. Lin, S., W. Chen, and S. S. Broyles. 1992. The vaccinia virus B1R gene product is a serine/threonine protein kinase. J. Virol. 66:2717-2723.
29. Liu, K., B. Lemon, and P. Traktman. 1995. The dual specificity phosphatase encoded by vaccinia virus, VH1, is essential for viral transcription in vivo and in vitro. J. Virol. 69:7823-7834.
30. Merchlinsky, M., and B. Moss. 1986. Resolution of linear minimichrosomes with hairpin ends from circular plasmids containing vaccinia virus concatemer junctions. Cell 45:879-884.
31. Mohandas, A. R., G. A. Dekaban, and S. Dales. 1994. Vaccinia virion surface polypeptide Ag35 expressed from a baculovirus vector is targeted to analogous poxvirus and insect virus components. Virology 200:207-219.
32. Moss, B. 1990. Poxviridae and their replication, p. 2079-2111. In B. N. Fields and D. M. Knipe (ed.), Virology. Raven Press, Inc., New York, N.Y.
33. Moss, B., and E. N. Rosenblum. 1973. Protein cleavage and poxvirus morphogenesis: tryptic peptide analysis of core precursors accumulated by blocking assembly with rifampicin. J. Mol. Biol. 81:267-269.
34. Nakano, E., D. Panicali, and E. Paoletti. 1982. Molecular genetics of vaccinia virus: demonstration of marker rescue. Proc. Natl. Acad. Sci. USA 79:1593-1596.
35. Ravanello, M. P., and D. E. Hruby. 1994. Conditional lethal expression of the vaccinia virus L1R myristylated protein reveals a role in virion assembly. J. Virol. 68:6401-6410.
36. Rempel, R. E., M. K. Anderson, E. Evans, and P. Traktman. 1990. Temperature-sensitive vaccinia virus mutants identify a gene with an essential role in viral replication. J. Virol. 64:574-583.
37. Rempel, R. E., and P. Traktman. 1992. Vaccinia virus B1 kinase: phenotypic analysis of temperature-sensitive mutants and enzymatic characterization of recombinant proteins. J. Virol. 66:4413-4426.
38. Rodriguez, D., M. Esteban, and J. R. Rodriguez. 1995. Vaccinia virus A17L gene product is essential for an early step in virion morphogenesis. J. Virol. 69:4640-4648.
39. Rodriguez, J. R., C. Risco, J. L. Carrascosa, M. Esteban, and D. Rodriguez. 1998. Vaccinia virus 15-kilodalton (A14L) protein is essential for assembly and attachment of viral crescents to virosomes. J. Virol. 72:1287-1296.
40. Rosel, J., P. L. Earl, J. Weir, and B. Moss. 1986. Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment. J. Virol. 60:436-449.
41. Rossi, G., A. Salminen, L. M. Rice, A. T. Brunger, and P. Brennwald. 1997. Analysis of a yeast SNARE complex reveals remarkable similarity to the neuronal SNARE complex and a novel function for the C terminus of the SNAP-25 homolog, Sec9. J. Biol. Chem. 272:16610-16617.
42. Sam, C.-K., and K. R. Dumbell. 1981. Expression of poxvirus DNA in coinfected cells and marker rescue of thermosensitive mutants by subgenomic fragments of DNA. Ann. Virol. 132:135-150.
43. Tartaglia, J., A. Piccini, and A. Paoletti. 1986. Vaccinia virus rifampicin-resistance locus specified a late 63,000 Da gene product. Virology 150:45-54.
44. Traktman, P., M. K. Anderson, and R. E. Rempel. 1989. Vaccinia virus encodes an essential gene with strong homology to protein kinases. J. Biol. Chem. 264:21458-21461.
45. Traktman, P., A. Caligiuri, S. A. Jesty, K. Liu, and U. Sankar. 1995. Temperature-sensitive mutants with lesions in the vaccinia virus F10 kinase undergo arrest at the earliest stage of virion morphogenesis. J. Virol. 69: 6581-6587.
46. Traktman, P., K. Liu, R. A. Rollins, S. A. Jesty, and B. Unger. Elucidating the essential rule of the A14 phosphoprotein in vaccinia virus morphogenesis: construction and characterization of a tetracycline-inducible recombinant. J. Virol., in press.
47. Wang, S., and S. Shuman. 1995. Vaccinia virus morphogenesis is blocked by temperature-sensitive mutations in the F10 gene, which encodes protein kinase 2. J. Virol. 69:6376-6388.
48. Weir, J. P., G. Bajszar, and B. Moss. 1982. Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell free translation of selected mRNA. Proc. Natl. Acad. Sci. USA 79:1210-1214.
49. Wertman, K. F., D. G. Drubin, and D. Holstein. 1992. Systematic mutational analysis of the yeast ACT1 gene. Genetics 132:337-350.
50. Williams, O., E. J. Wolffe, A. S. Weisberg, and M. Merchlinsky. 1999. Vaccinia virus WR gene A5L is required for morphogenesis of mature virions. J. Virol. 73:4590-4599.
51. Wolffe, E. J., D. M. Moore, E. J. Peters, and B. Moss. 1996. Vaccinia virus A17L open reading frame encodes an essential component of nascent viral membranes that is required to initiate morphogenesis. J. Virol. 70:2797-2808.
52. Zhang, Y., J. G. Keck, and B. Moss. 1992. Transcription of viral late genes is dependent on expression of the viral intermediate gene G8R in cells infected with an inducible conditional-lethal mutant vaccinia virus. J. Virol. 66:6470-6479.
53. Zhang, Y., and B. Moss. 1991. Vaccinia virus morphogenesis is interrupted when expression of a gene encoding an 11-kilodalton phosphorylated protein is prevented by the Escherichia coli lac repressor. J. Virol. 65:6101-6110.
54. Zhang, Y., and B. Moss. 1992. Immature viral envelope formation is interrupted at the same stage by lac operator mediated repression of the vaccinia virus D13L gene and by the drug rifampicin. Virology 187:643-653.