Phenotypic characterization of mutants in vaccinia virus gene G2R, a putative transcription elongation factor

Journal of Virology

Volumn 70, Issue 1, 1996, Pages 47-54

  Penelope Black, E ^a   Condit, Richard C ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

ANIMAL CELL; ARTICLE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; TRANSCRIPTION REGULATION; VACCINIA VIRUS; VIRUS GENE;

EID: 0029655746     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.1.47-54.1996     Document Type: Article

References (28)

1. Baldick, C. J., Jr., J. G. Keck, and B. Moss. 1992 Mutational analysis of the core, spacer, and initiator regions of vaccinia virus intermediate-class promoters. J. Virol 66:4710-4719.
2. Baldick, C. J., Jr. and B. Moss. 1993. Characterization and temporal regulation of mRNAs encoded by vaccinia virus intermediate-stage genes. J Virol. 67:3515-3527.
3. Bayliss, C. D., and R. C. Condit. 1993. Temperature-sensitive mutants in the vaccinia virus A18R gene increase double-stranded RNA synthesis as a result of aberrant viral transcription. Virology 194:254-262.
4. Condit, R. C., R. Easterly, R. F. Pacha, Z. Fathi, and R. J. Meis. 1991. A vaccinia virus isatin-β-thiosemicarbazone resistance mutation maps, in the viral gene encoding the 132-kDa subunit of RNA polymerase. Virology 185:857-861.
5. Condit, R. C., and A. Motyczka. 1981. Isolation and preliminary characterization of temperature-sensitive mutants of vaccinia virus. Virology 113:224-241.
6. Condit, R. C., A. Motyczka, and G. Spizz. 1983 Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus Virology 128:429-443.
7. Condit, R. C., and E. G. Niles. 1990. Orthopoxvirus genetics. Curr. Top Microbiol. Immunol. 163:1-40.
8. Condit, R. C., Y. Xiang, and J. I. Lewis. 1995. Unpublished data.
9. 1 subunit of the virion-associated DNA-dependent RNA polymerase. J. Virol. 61:1842-1850.
10. Feinberg, A. P., and B. Vogelstein. 1984 A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Addendum. Anal Biochem. 137:266-267.
11. Hassett, D. E., and R. C. Condit. 1994. Targeted construction of temperature-sensitive mutations in vaccinia virus by replacing clustered charged residues with alanine Proc. Natl. Acad. Sci. USA 91:4554-4558.
12. Kane, C. M. 1994. Transcript elongation and gene regulation in cukaryotes. p. 279-296. In R C. Conaway and J. W. Conaway (ed.), Transcription mechanisms and regulation. Raven Press, New York.
13. Kovacs, G. R., R. Rosales, J. G. Keck, and B. Moss. 1994. Modification of the cascade model for regulation of vaccinia virus gene expression: purification of a prereplicative, late-stage-specific transcription factor. J Virol. 68:3443-3447.
14. Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
15. Mahr, A., and B. E. Roberts. 1984. Arrangement of late RNAs transcribed from a 7.1-kilobase EcoRI vaccinia virus DNA fragment. J. Virol. 49:510-520.
16. Meis, R. J. 1990 Molecular biological and phenotypic characterization of a vaccinia virus gene which renders the virus dependent on isatin-beta-thiosemicarbazone (IBT). PhD thesis. State University of New York, Buffalo
17. Meis, R. J., and R. C. Condit. 1991. Genetic and molecular biological characterization of a vaccinia virus gene which renders the virus dependent on isatin-β-thiosermcarbazone (IBT). Virology 182:442-454.
18. Milligan, J. F., D. R. Groebe, G. W. Witherell, and O. C. Uhlenbeck. 1987 Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 15:8783-8798.
19. Moss, B. 1990 Poxviridae and their replication, p. 2079-2112. In B. N. Fields, D M Knipe, R M Chanock, M. S. Hirsch, J. L. Melnick, T. P. Monath, and B. Roizman (ed.), Virology. Raven Press, New York.
20. Moss, B., B. Y. Ahn, B. Amegadzie, P. D. Gershon, and J. G. Keck. 1991 Cytoplasmic transcription system encoded by vaccinia virus. J. Biol. Chem 266:1355-1358.
21. Moyer, R. W. 1987. The role of the host cell nucleus in vaccinia virus morphogenesis. Virus Res. 8:173-191
22. Pacha, R. F., and R. C. Condit. 1985. Characterization of a temperature-sensitive mutant of vaccinia virus reveals a novel function that prevents virus-induced breakdown of RNA. J. Virol. 56:395-403.
23. Rosales, R., N. Harris, B. Y. Ahn, and B. Moss. 1994. Purification and identification of a vaccinia virus-encoded intermediate stage promoter-specific transcription factor that has homology to eukaryotic transcription factor SII (TFIIS) and an additional role as a viral RNA polymerase subunit. J Biol. Chem. 269:14260-14267.
24. Rosales, R., G. Sutter, and B. Moss. 1994 A cellular factor is required for transcription of vaccinia viral intermediate-stage genes. Proc. Natl. Acad Sci. USA 91:3794-3798.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. Shuman, S., S. S. Broyles, and B. Moss. 1987. Purification and characterization of a transcription termination factor from vaccinia virions. J. Biol. Chem. 262:12372-12380.
27. Simpson, D. A., and R. C. Condit. 1995. Vaccinia virus gene A18R encodes an essential DNA helicase J. Virol. 69:6131-6139
28. Yuen, L., and B. Moss. 1987. Oligonucleotide sequence signaling transcriptional termination of vaccinia virus early genes. Proc. Natl. Acad Sci. USA. 84:6417-6421.