The regulator of the yeast proline utilization pathway is differentially phosphorylated in response to the quality of the nitrogen source

Molecular and Cellular Biology

Volumn 20, Issue 3, 2000, Pages 892-899

  Huang, Hoching L ^a   Brandriss, Marjorie C ^a  

^a RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; NITROGEN; PERMEASE; PHOSPHATASE; PHOSPHOPROTEIN; PHOSPHORUS 32; PHOSPHOTRANSFERASE; PROLINE; PROTEIN PUT3P; SULFUR 35; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; NITROGEN METABOLISM; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PHOSPHORYLATION; REGULATOR GENE; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION REGULATION;

CULTURE MEDIA; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; NITROGEN; PHOSPHOPROTEINS; PHOSPHORYLATION; PROLINE; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC;

EID: 0033958305     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.20.3.892-899.2000     Document Type: Article

References (50)

1. Axelrod, J. D., J. Majors, and M. C. Brandriss. 1991. Proline-independent binding of PUT3 transcriptional activator protein detected by footprinting in vivo. Mol. Cell. Biol. 11:564-567.
2. Bisson, L. F. 1991. Influence of nitrogen on yeast and fermentation of grapes, p. 78-89. In Proceedings of the International Symposium on Nitrogen in Grapes and Wine. American Society for Enology and Viticulture, Davis, Calif.
3. Blinder, D., P. E. Coschigano, and B. Magasanik. 1996. Interaction of the GATA factor Gln3p with the nitrogen regulator Ure2p in Saccharomyces cerevisiae. J. Bacteriol. 178:4734-4736.
4. Brandriss, M. C. 1987. Evidence for positive regulation of proline utilization in Saccharomyces cerevisiae. Genetics 117:429-435.
5. Brandriss, M. C. 1979. Isolation and preliminary characterization of Saccharomyces cerevisiae proline auxotrophs. J. Bacteriol. 138:816-822.
6. Brandriss, M. C., and B. Magasanik. 1979. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: enzyme induction by proline. J. Bacteriol. 140:498-503.
7. Brandriss, M. C., and B. Magasanik. 1979. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: mutation causing constitutive enzyme expression. J. Bacteriol. 140:504-507.
8. Chasman, D. I., and R. D. Kornberg. 1990. Gal4 protein: purification, association with Gal80 protein, and conserved domain. Mol. Cell. Biol. 10:2916-2923.
9. Coffman, J. A., R. Rai, and T. G. Cooper. 1995. Genetic evidence for Gln3p-independent, nitrogen catabolite repression-sensitive gene expression in Saccharomyces cerevisiae. J. Bacteriol. 177:6910-6918.
10. Coffman, J. A., R. Rai, T. Cunningham, V. Svetlov, and T. G. Cooper. 1996. Gat1p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae. Mol. Cell. Biol. 16:847-858.
11. Coffman, J. A., R. Rai, D. M. Loprete, T. Cunningham, V. Svetlov, and T. G. Cooper. 1997. Cross regulation of four GATA factors that control nitrogen catabolic gene expression in Saccharomyces cerevisiae. J. Bacteriol. 179:3416-3429.
12. Coornaert, D., S. Vissers, B. Andre, and M. Grenson. 1992. The UGA43 negative regulatory gene of Saccharomyces cerevisiae contains both a GATA-1 type zinc finger and a putative leucine zipper. Curr. Genet. 21:301-307.
13. Cunningham, T. S., and T. G. Cooper. 1991. Expression of DAL80, whose product is homologous to the GATA factors and is a negative regulator of multiple nitrogen catabolic genes in Saccharomyces cerevisiae, is sensitive to nitrogen catabolite repression. Mol. Cell. Biol. 11:6205-6215.
14. Daugherty, J. R., R. Rai, H. El Berry, and T. G. Cooper. 1993. Regulatory circuit for responses of nitrogen catabolic gene expression to the GLN3 and DAL80 proteins and nitrogen catabolite repression in Saccharomyces cerevisiae. J. Bacteriol. 175:64-73.
15. des Etages, S. G., D. A. Falvey, R. J. Recce, and M. C. Brandriss. 1996. Functional analysis of the PUT3 transcriptional activator of the proline utilization pathway in Saccharomyces cerevisiae. Genetics 142:1069-1082.
16. Hedges, D., M. Proft, and K.-D. Entian. 1995. CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 15:1915-1922.
17. Jauniaux, J.-C., M. Vandenbol, S. Vissers, K. Broman, and M. Grenson. 1987. Nitrogen catabolite regulation of proline permease in Saccharomyces cerevisiae. Cloning of the PUT4 gene and study of PUT4 RNA levels in wild-type and mutant strains. Eur. J. Biochem. 164:601-606.
18. Johnston, M., and R. W. Davis. 1984. Sequences that regulate the divergent GAL1-10 promoter in Saccharomyces cerevisiae. Mol. Cell. Biol. 4:1440-1448.
19. Jones, E. W. 1991. Tackling the protease problem in Saccharomyces cerevisiae. Methods Enzymol. 194:428-453.
20. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
21. Lasko, P., and M. C. Brandriss. 1981. Proline transport in Saccharomyces cerevisiae. J. Bacteriol. 148:241-247.
22. 6 zinc cluster transcriptional activator: a new role for SNF1 in the glucose response. Mol. Cell. Biol. 16:1921-1928.
23. Magasanik, B. 1992. Regulation of nitrogen utilization, p. 283-317. In E. W. Jones, J. R. Pringle, and J. R. Broach (ed.), The molecular and cellular biology of the yeast Saccharomyces, vol. 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
24. Marczak, J. E., and M. C. Brandriss. 1991. Analysis of constitutive and noninducible mutations of the PUT3 transcriptional activator. Mol. Cell. Biol. 11:2609-2619.
25. Marczak, J. E., and M. C. Brandriss. 1989. Isolation of constitutive mutations affecting the proline utilization pathway in Saccharomyces cerevisiae and a molecular analysis of the PUT3 transcriptional activator. Mol. Cell. Biol. 9:4696-4705.
26. Mitchell, A. P., and B. Magasanik. 1984. Regulation of glutamine-repressible gene products by the GLN3 function in Saccharomyces cerevisiae. Mol. Cell. Biol. 4:2758-2766.
27. Mylin, L. M., P. J. Bhat, and J. E. Hopper. 1989. Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator. Genes Dev. 3:1157-1165.
28. Mylin, L. M., M. Johnston, and J. E. Hopper. 1990. Phosphorylated forms of GAL4 are correlated with ability to activate transcription. Mol. Cell. Biol. 10:4623-4629.
29. Okajima, T., T. Tanabe, and T. Yasuda. 1993. Nonurea sodium dodecyl sulfate-polyacrylamide gel electrophoresis with high-molarity buffers for the separation of proteins and peptides. Anal. Biochem. 211:293-300.
30. Orr-Weaver, T. L., J. Szostak, and R. Rothstein. 1983. Genetic applications of yeast transformation with linear and gapped plasmids. Methods Enzymol. 101:228-245.
31. Parthun, M. R., and J. A. Jaehning. 1992. A transcriptionally active form of GAL4 is phosphorylated and associated with GAL80. Mol. Cell. Biol. 12: 4981-4987.
32. Randez-Gil, F., N. Bojunga, M. Proft, and K.-D. Entian. 1997. Glucose derepression of gluconeogenic enzymes in Saccharomyces cerevisiae correlates with phosphorylation of the gene activator Cat8p. Mol. Cell. Biol. 17:2502-2510.
33. Reece, R. J., and M. Ptashne. 1993. Determinants of binding site-specificity among yeast C6 zinc cluster proteins. Science 261:909-911.
34. Rowen, D. W., N. Esiobu, and B. Magasanik. 1997. Role of GATA factor Nil2p in nitrogen regulation of gene expression in Saccharomyces cerevisiae. J. Bacteriol. 179:3761-3766.
35. Sadowski, I., C. Costa, and R. Dhanawansa. 1996. Phosphorylation of Gal4p at a single C-terminal residue is necessary for galactose-inducible transcription. Mol. Cell. Biol. 16:4879-4887.
36. Sadowski, I., D. Neidbala, K. Wood, and M. Ptashne. 1991. GAL4 is phosphorylated as a consequence of transcriptional activation. Proc. Natl. Acad. Sci. USA 88:10510-10514.
37. 6 zinc cluster family of transcriptional regulators. Nucleic Acids Res. 24:4599-4607.
38. Schmidt, A., T. Beck, A. Koller, J. Kunz, and M. N. Hall. 1998. The TOR nutrient signalling pathway phosphorylates NPR1 and inhibits turnover of the tryptophan permease. EMBO J. 17:6924-6931.
39. Siddiqui, A. H., and M. C. Brandriss. 1988. A regulatory region responsible for proline-specific induction of the yeast PUT2 gene is adjacent to its TATA box. Mol. Cell. Biol. 8:4634-4641.
40. Siddiqui, A. H., and M. C. Brandriss. 1989. The Saccharomyces cerevisiae PUT3 activator protein associates with proline-specific upstream activation sequences. Mol. Cell. Biol. 9:4706-4712.
41. Soussi-Boudekou, S., and B. Andre. 1999. A co-activator of nitrogen-regulated transcription in Saccharomyces cerevisiae. Mol. Microbiol. 31:753-762.
42. Soussi-Boudekou, S., S. Vissers, A. Urrestarazu, J.-C. Jauniaux, and B. Andre. 1997. Gzf3p, a fourth GATA factor involved in nitrogen-regulated transcription in Saccharomyces cerevisiae. Mol. Microbiol. 23:1157-1168.
43. Stanbrough, M., and B. Magasanik. 1995. Transcriptional and posttranslational regulation of the general amino acid permease of Saccharomyces cerevisiae. J. Bacteriol. 177:94-102.
44. Stanbrough, M., D. W. Rowen, and B. Magasanik. 1995. Role of the GATA factors Gln3p and Nil1p of Saccharomyces cerevisiae in the expression of nitrogen-regulated genes. Proc. Natl. Acad. Sci. USA 92:9450-9454.
45. 6 binuclear cluster. Nat. Struct. Biol. 4:751-759.
46. Tomenchok, D. M., and M. C. Brandriss. 1987. Gene-enzyme relationships in the proline biosynthesis pathway of Saccharomyces cerevisiae. J. Bacteriol. 169:5346-5372.
47. Towbin, H., J. Stachelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
48. Vandenbol, M., J.-C. Jauniaux, and M. Grenson. 1990. The Saccharomyces cerevisiae NPR1 gene required for the activity of ammonia-sensitive amino acid permeases encodes a protein kinase homologue. Mol. Gen. Genet. 222:393-399.
49. Walters, K. J., K. T. Dayie, R. J. Reece, M. Ptashne, and G. Wagner. 1997. Structure and mobility of the PUT3 dimer. Nat. Struct. Biol. 4:744-750.
50. Xu, S., D. A. Falvey, and M. C. Brandriss. 1995. Roles of URE2 and GLN3 in the proline utilization pathway in Saccharomyces cerevisiae. Mol. Cell. Biol. 15:2321-2330.