Establishment and characterization of a culture system for enzymatically released rat dental pulp cells

Calcified Tissue International

Volumn 66, Issue 2, 2000, Pages 139-144

  Yokose, S ^a   Kadokura, H ^a   Tajima, Y ^a   Fujieda, K ^a   Katayama, I ^a   Matsuoka, T ^a   Katayama, T ^a  

^a MEIKAI UNIVERSITY SCHOOL OF DENTISTRY   (Japan)

Author keywords

Cell culture; Dental pulp cells; Differentiation; Odontoblast like cells

Indexed keywords

ALKALINE PHOSPHATASE; CALCIUM; GLYCOSAMINOGLYCAN POLYSULFATE; MESSENGER RNA; OSTEOCALCIN; PARATHYROID HORMONE; PARATHYROID HORMONE RELATED PROTEIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CULTURE MEDIUM; DENTIN; ENZYME ACTIVITY; INCUBATION TIME; MINERALIZATION; NONHUMAN; ODONTOBLAST; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TOOTH PULP;

ALKALINE PHOSPHATASE; ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL SEPARATION; CELLS, CULTURED; DENTAL PULP; GENE EXPRESSION REGULATION; GLYCOSAMINOGLYCANS; ODONTOBLASTS; OSTEOCALCIN; RATS; RATS, SPRAGUE-DAWLEY; RECEPTOR, PARATHYROID HORMONE, TYPE 1; RECEPTORS, PARATHYROID HORMONE; RNA, MESSENGER; TIME FACTORS; TRANSCRIPTION, GENETIC;

EID: 0033955416     PISSN: 0171967X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002230010028     Document Type: Article

References (40)

1. Whitoson SW (1998) Dentin-pulp complex. In: Ten Cate AR (ed) Oral histology: development, structure, and function, 5th ed. Mosby, St. Louis, pp 150-196
2. Young WG, Ruch JV, Stevens MR, Begu-Kirn C, Zhang CZ, Lesot H, Waters MJ (1995) Comparison of the effects of growth hormone, insulin-like growth factor-I and fetal calf serum on mouse molar odontogenesis in vitro. Arch Oral Biol 40:789-799
3. Tsukamoto Y, Fukutani S, Shin-Ike T, Kubota T, Sato S, Suzuki Y, Mori M (1992) Mineralized nodule formation by cultures of human dental pulp-derived fibroblasts. Arch Oral Biol 37:1045-1055
4. Nakashima M (1991) Establishment of primary cultures of pulp cells from bovine permanent incisors. Arch Oral Biol 36:655-663
5. Shiba H, Nakamura S, Shirakawa M, Nakanishi K, Okamoto H, Satakeda H, Noshiro M, Kamihagi K, Katayama M, Kata Y (1955) Effects of basic fibroblast growth factor on proliferation, the expression of osteonectin (SPARC) and alkaline phosphatase, and calcification in cultures of human pulp cells. Dev Biol 170:457-466
6. Neubuser A, Peters H, Balling R, Martin GR (1997) Antagonistic interactions between FGF and BMP signaling pathways: a mechanism for positioning the sites of tooth formation. Cell 90:247-255
7. Nakashima M (1994) Induction of dentin formation on canine amputated pulp by recombinant human bone morphogenetic proteins (BMP)-2 and -4. J Dent Res 73:1515-1522
8. Shirakawa M, Shiba H, Nakanishi K, Ogawa T, Okamoto H, Nakashima K, Noshiro M, Kata Y (1994) Transforming growth factor-beta-1 reduces alkaline phosphatase mRNA and activity and stimulates cell proliferation in cultures of human pulp cells. J Dent Res 73:1509-1514
9. Kasugai S, Adachi M, Ogura H (1988) Establishment and chracterization of a clonal cell line (RPC-C2A) from dental pulp of the rat incisor. Arch Oral Biol 33:887-891
10. Kawase T, Orikasa M, Ogata S, Burns D (1995) Protein tyrosine phosphorylation induced by epidermal growth factor and insulin-like growth factor-I in a rat clonal dental pulp-cell line. Arch Oral Biol 40:921-929
11. Satoyoshi M, Koizumi T, Teranaka T, Iwamoto T, Takita H, Saito S, Mikuni-Tkagaki Y (1995) Extracellular processing of dentin matrix protein in the mineralizing odontoblast culture. Calcif Tissue Int 57:237-241
12. Waddington RJ, Embery G, Hall RC (1993) The influence of fluoride on proteoglycan structure using a rat odontoblast in vitro system. Calcif Tissue Int 52:392-398
13. Wada Y, Kataoka H, Yokose S, Ishizuya T, Miyazono K, Gao YH, Shibasaki Y, Yamaguchi A (1998) Changes in osteoblast phenotype during differentiation of enzymatically isolated rat calvaria cells. Bone 22:479-485
14. Yokose S, Ishizuya T. Ikeda T, Nakamura T, Tsurukami H, Kaeasaki K, Suda T, Yoshiki S, Yamaguchi A (1996) An estrogen deficiency caused by ovariectomy increases plasma levels of systemic factors that stimulate proliferation and differentiation of osteoblasts in rats. Endocrinology 137:469-478
15. Dare A, Hachisu R, Yamaguchi A, Yokose S, Yoshiki S, Okano T (1997) Effects of ionizing radiation on proliferation and differentiation of osteoblast-like cells. J Dent Res 76:658-664
16. Asahina I, Sampath TK, Nishimura I, Hauschka PV (1993) Human osteogenic protein-1 induces both chondroblastic and osteoblastic differentiation of osteoprogenitor cells derived from newborn rat calvaria. J Cell Biol 123:921-933
17. Ishizuya T, Yokose S, Hori M, Noda T, Suda T, Yoshiki S, Yamaguchi A (1997) Parathyroid hormone exerts disparate effects on osteoblast differentiation depending on exposure time in rat osteoblastic cells. J Clin Invest 99:2961-2970
18. George A, Sabsay B, Simonian PAL, Veis A (1993) Characterization of a novel dentin matrix acidic phosphoprotein. J Biol Chem 268:12624-12630
19. Miller WA, Everett MM, Cramer JF (1976) Growth of bovine pulp cells in monolayer culture. J Endod 2:385-389
20. Nuki K, Bonting SL (1961) Quantitative histochemistry of the developing hamster tooth: alkaline phosphatase and lactic dehydrogenase. J Histochem Cytochem 9:117-125
21. Goggins JF, Fullmer HM (1967) Hydrolytic enzyme histochemistry of the rat molar pulp. Arch Oral Biol 12:639-644
22. Yoshiki S, Kurahashi Y (1971) A light and electron microscopic study of alkaline phosphatase activity in the early stage of dentinogenesis in the young rat. Arch Oral Biol 16:1143-1154
23. Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS (1990) Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationship in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143:420-430
24. Nakashima M (1992) The effects of growth factors on DNA synthesis, proteoglycan synthesis, and alkaline phosphatase activity in bovine dental pulp cell. Arch Oral Biol 37:231-236
25. Bouvier M, Joffre A, Magloire H (1990) In vitro mineralization of a three-dimensional collagen matrix by human dental pulp cells in the presence of chondroitin sulphate. Arch Oral Biol 35:301-309
26. Chung C-H, Golub EE, Forbes E, Tokuoka T, Shapiro IM (1992) Mechanism of action of β-glycerophosphate on bone cell mineralization. Calcif Tissue Int 51:305-311
27. Gundberg CM, Hauschka PV, Lian JB, Gallop PM (1984) Osteocalcin: isolation, characterization and detection. Methods Enzymol 107:516-544
28. Geroge A, Silberstein R, Veis A (1995) In situ hybridization shows DMP-1 (AG1) to be a developmentally regulated dentin-specific protein produced by mature odontoblasts. Connect Tissue Res 33:67-72
29. Macdougall M, Gu TT, Luan X, Simmons D, Chen J (1998) Identification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissues. J Bone Miner Res 13:422-431
30. Linde A (1984) Noncollagenous proteins and proteoglycans in dentinogenesis. In: Linde A (ed) Dentin and dentinogenesis, vol II. CRC Press, Boca Raton, Florida, pp 55-92
31. Bidder M, Latifi T, Towler DA (1998) Reciprocal temporospatial patterns of Msx2 and osteocalcin gene expression during murine odontogenesis. J Bone Miner Res 13:609-619
32. Ten Cate AR (1998) Dentinogenesis. In: Ten Cate AR (ed) Oral histology: development, structure, function. Mosby, St. Louis, pp 128-149
33. Clark RD, Smith JG, Davidson EA (1965) Hexosamine and acidic glycosaminoglycans in human teeth. Biochim Biophys Acta 101:267-272
34. Jones IL, Leaver AG (1974) Glycosaminoglycans of human dentin. Calcif Tissue Res 16:37-44
35. Nicholson WAP, Ashton BA, Hohling HJ, Quint P, Schreiber J (1977) Electron microprobe investigations into the process of hard tissue formation. Cell Tissue Res 177:331-345
36. Pugliarello MC, Vittur F, Bernard B, Bonucci E, Ascenzi A (1970) Chemical modifications in osteons during calcification. Calcif Tissue Res 5:108-114
37. Baylink D, Wergedal J, Thompson E (1972) Loss of proteoglycan saccharides at site where bone minerlization is initiated. J Histochem Cytochem 20:279-292
38. Lee K, Deeds JD, Segre G (1995) Expression of parathyroid hormone-related peptide and its receptor messenger ribonucleic acids during fetal development of rats. Endocrinology 136:453-463
39. Karaplis AC, Luz A, Glowacki J. Bronson RT, Tybulewicz VLJ, Kronenberg HM, Mulligan RC (1993) Lethal skeletal dysplasia from targeted disruption of the parathyroid hormone-related peptide gene. Genes Dev 8:277-289
40. Lundgren T, Stenport V, Wetter A, Linde A (1998) Parathyroid horrmone (1-34) receptor-binding and second-messenger response in rat incisor odontoblasts. Calcif Tissue Int 62:255-259