A simplified procedure for the preparation of MHC/peptide tetramers: Chemical biotinylation of an unpaired cysteine engineered at the C-terminus of MHC-I

Journal of Immunological Methods

Volumn 234, Issue 1-2, 2000, Pages 61-70

  Kalergis, Alexis M ^a   Goyarts, Earl C ^a   Palmieri, Edith ^a   Honda, Shinichiro ^a   Zhang, Weijia ^a   Nathenson, Stanley G ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Cysteine biotinylation; MHC I tetramers; TCR activation; TCR cross reactivity; TCR specificity

Indexed keywords

CYSTEINE; INTERLEUKIN 2; MAJOR HISTOCOMPATIBILITY ANTIGEN; T LYMPHOCYTE RECEPTOR; TETRAMER;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BIOTINYLATION; CARBOXY TERMINAL SEQUENCE; CROSS REACTION; CYTOTOXIC T LYMPHOCYTE; DISSOCIATION; HYBRIDOMA; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; RECEPTOR AFFINITY; T LYMPHOCYTE ACTIVATION;

BETA 2-MICROGLOBULIN; BIOTINYLATION; CROSS REACTIONS; CYSTEINE; GENETIC ENGINEERING; H-2 ANTIGENS; INTERLEUKIN-2; LYMPHOCYTE ACTIVATION; NUCLEOCAPSID; NUCLEOCAPSID PROTEINS; PEPTIDES; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; T-LYMPHOCYTES; T-LYMPHOCYTES, CYTOTOXIC;

EID: 0033951457     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00215-X     Document Type: Article

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