IL-12 induces SDS-stable class II αβ dimers in murine dendritic cells

Cytokine

Volumn 12, Issue 4, 2000, Pages 401-404

  Grohmann, U ^a   Orabona, C ^a   Bianchi, R ^a   Belladonna, M L ^a   Fioretti, M C ^a   Puccetti, P ^a  

^a UNIVERSITY OF PERUGIA   (Italy)

Author keywords

Dendritic cells; IL 12; MHC class II; SDS stability

Indexed keywords

DIMER; DODECYL SULFATE SODIUM; HLA ANTIGEN CLASS 2; INTERLEUKIN 12;

ANIMAL CELL; ANTIGEN EXPRESSION; ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; DENDRITIC CELL; IN VITRO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL;

EID: 0033949016     PISSN: 10434666     EISSN: None     Source Type: Journal    
DOI: 10.1006/cyto.1999.0558     Document Type: Article

References (14)

1. Germain RN, Hendrix LR. MHC class II structure, occupancy and surface expression determined by post-endoplasmic reticulum antigen binding. Nature. 353:1991;134-139.
2. kα-chain required for the binding and stability of two antigenic peptides. J Immunol. 156:1996;176-182.
3. g7molecules from non-obese diabetic mice are poor peptide binders. J Immunol. 156:1996;450-458.
4. Bianchi R, Grohmann U, Belladonna ML, Silla S, Fallarino F, Ayroldi E, Fioretti MC, Puccetti P. IL-12 is both required and sufficient for initiating T cell reactivity to a class I-restricted tumor peptide (P815AB) following transfer of P815AB-pulsed dendritic cells. J Immunol. 157:1996;1589-1597.
5. Grohmann U, Bianchi R, Ayroldi E, Belladonna ML, Surace D, Fioretti MC, Puccetti P. A tumor-associated and self antigen peptide presented by dendritic cells may induce T cell anergy in vivo, but IL-12 can prevent or revert the anergic state. J Immunol. 158:1997;3593-3602.
6. +lymphocytes in the initiation of class I-restricted reactivity to a tumor/self peptide. Crit Rev Immunol. 18:1998;87-98.
7. Grohmann U, Belladonna ML, Bianchi R, Orabona C, Ayroldi E, Fioretti MC, Puccetti P. IL-12 acts directly on DC to promote nuclear localization of NF-κB and primes DC for IL-12 production. Immunity. 9:1998;315-323.
8. g7protein of non-obese diabetic mice. Eur J Immunol. 27:1997;2478-2483.
9. Cella MA, Engering A, Pinet V, Pieters J, Lanzavecchia A. Inflammatory stimuli induce accumulation of MHC class II complexes on dendritic cells. Nature. 388:1997;782-787.
10. Pierre P, Mellman I. Developmental regulation of invariant chain proteolysis controls MHC class II trafficking in mouse dendritic cells. Cell. 93:1998;1135-1145.
11. Banchereau J, Steinman RM. Dendritic cells and the control of immunity. Nature. 392:1998;245-252.
12. Trinchieri G. Interleukin-12: a proinflammatory cytokine with immunoregulatory functions that bridges innate resistance and antigen-specific adaptive immunity. Annu Rev Immunol. 13:1995;251-276.
13. Nelson CA, Petzold SJ, Unanue ER. Identification of two distinct properties of class II major histocompatibility complex-associated peptides. Proc Natl Acad Sci U S A. 90:1993;1227-1231.
14. Grohmann U, Ullrich SJ, Mage MG, Appella E, Fioretti MC, Puccetti P, Romani L. Identification and immunogenic properties of an 80-kDa surface antigen on a drug-treated tumor variant: relationship to MuLV gp70. Eur J Immunol. 20:1990;629-636.