Production of functional human selenocysteine-containing KDRF/thioredoxin reductase in E. coli

Journal of Biochemistry

Volumn 127, Issue 6, 2000, Pages 977-983

  Koishi, Ryuta ^a   Nakamura, Takemichi ^a   Takazawa, Tomoko ^a   Yoshimura, Chigusa ^a   Serizawa, Nobufusa ^a  

^a DAIICHI SANKYO CO   (Japan)

Author keywords

KDRF; LC ESI MS; Selenium; Selenocysteine; Thioredoxin reductase

Indexed keywords

COMPLEMENTARY DNA; FORMATE DEHYDROGENASE; SELENOCYSTEINE; THIOREDOXIN REDUCTASE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL PROTECTION; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; HUMAN; HUMAN CELL; LUNG ADENOCARCINOMA; NONHUMAN; PROTEIN EXPRESSION; SEQUENCE ANALYSIS;

ESCHERICHIA COLI; MAMMALIA;

EID: 0033948058     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022714     Document Type: Article

References (35)

1. Van Vleet, J.F. and Ferrans, V.J. (1992) Etiologic factors and pathologic alterations in selenium-vitamin E deficiency and excess in animals and humans. Biol. Trace Elem. Res. 33, 1-21
2. Bock, A., Forchhammer, K., Heider, J., Leinfelder, W., Sawers, G., Veprek, B., and Zinoni, F. (1991) Selenocysteine: the 21st amino acid. Mol. Microbiol. 5, 515-520
3. Stadtman, T.C. (1990) Selenium biochemistry. Annu. Rev. Biochem. 59, 111-127
4. Bock, A., Forchhammer, K., Heider, J., and Baron, C. (1991) Selenoprotein synthesis: an expansion of the genetic code. Trends Biochem. Sci. 16, 463-467
5. Zinoni, F., Heider, J., and Bock, A. (1990) Features of the formate dehydrogenase mRNA necessary for decoding of the UGA codon as selenocysteine. Proc. Natl. Acad. Sci. USA 87, 4660-4664
6. Berg, B.L., Baron, C., and Stewart, V. (1991) Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. J. Biol. Chem. 266, 22386-22391
7. Heider, J., Baron, C., and Bock, A. (1992) Coding from a distance: dissection of the mRNA determinants required for the incorporation of selenocysteine into protein. EMBO J. 11, 3759-3766
8. Berry, M.J., Banu, L., Harney, J.W., and Larsen, P.R. (1993) Functional characterization of the eukaryotic SECIS elements which direct selenocysteine insertion at UGA codons. EMBO J. 12, 3315-3322
9. Berry, M.J., Banu, L., Chen, Y., Mandel, S.J., Kieffer, J.D., Harney, J.W., and Larsen, P.R. (1991) Recognition of UGA as a selenocysteine codon in Type I deiodinase requires sequences in the 3′ untranslated region. Nature 353, 273-276
10. Rocher, C., Faucheu, C., Herve, F., Benicourt, C., and Lalanne, J-L. (1991) Cloning of murine SeGpx cDNA and synthesis of mutated GPx proteins in Escherichia coli. Gene 98, 193-200
11. Koishi, R., Kawashima, I., Yoshimura, C., Sugawara, M., and Serizawa, N. (1997) Cloning and characterization of a novel oxidoreductase KDRF from a human bone marrow-derived stromal cell line KM-102. J. Biol. Chem. 272, 2570-2577
12. Gasdaska, P.Y., Gasdaska, J.R., Cochran, S., and Powis, G. (1995) Cloning and sequencing of a human thioredoxin reductase. FEBS Lett. 373, 5-9
13. Miranda-Vizuete, A. and Spyrou, G. (1997) The novel oxidoreductase KDRF (KM-102-derived reductase-like factor) is identical with human thioredoxin reductase. Biochem. J. 325, 287-288
14. Moore, E.G., Reichard, P., and Thelander, L. (1964) Enzymic synthesis of deoxyribonudeotides. J. Biol. Chem. 239, 3445-3452
15. Bjornstedt, M., Hamberg, M., Kumar, S., Xue, J., and Holmgren, A. (1995) Human thioredoxin reductase directly reduces lipid hydroperoxides by NADPH and selenocysteine strongly stimulates the reaction via catalytically generated selenols. J. Biol. Chem. 270, 11761-11764
16. Mitsui, A., Hirakawa, T., and Yodoi, J. (1992) Reactive oxygen-reducing and protein-refolding activities of adult T cell leukemia-derived factor/human thioredoxin. Biochem. Biophys. Res. Commun. 186, 1220-1226
17. Schallreuter, K.U. and Wood, J.M. (1986) The role of thioredoxin reductase in the reduction of free radicals at the surface of the epidermis. Biochem. Biophys. Res. Commun. 136, 630-637
18. Stadtman, E.R. (1992) Protein oxidation and aging. Science 257, 1220-1224
19. Cross, C.E. (1987) Oxygen radicals and human disease. Ann. Intern. Med. 107, 526-545
20. Cross, A.R. (1990) Inhibitors of the leukocyte superoxide generating oxidase: mechanisms of action and methods for their elucidation. Free Radical Biol. Med. 8, 71-93
21. Tamura, T. and Stadtman, T.C. (1996) A new selenoprotein from human lung adenocarcinoma cells: purification, properties, and thioredoxin reductase activity. Proc. Natl. Acad. Sci. USA 93, 1006-1011
22. Gladyshev, V.N., Jeang, K-T., and Stadtman, T.C. (1996) Selenocysteine, identified as the penultimate C-terminal residue in human T-cell thioredoxin reductase, corresponds to TGA in the human placental gene. Proc. Natl. Acad. Sci. USA 93, 6146-6151
23. Takahashi, T., Nakanishi, M., Yao, Y., Uyeda, I., and Serizawa, N. (1998) Direct formation of human interleukin-11 by cis-acting system of plant virus protease in Escherichia coli. Biosci. Biotechnol. Biochem. 62, 953-958
24. Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982) Molecular Cloning:A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
25. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
26. Hellman, U., Wernstedt, C., Gonez, J., and Heldin, C-H. (1995) Improvement of an "In-Gel" digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing. Anal. Biochem. 224, 451-455
27. Shevchenko, A., Wilm, M., Vorm, O., and Mann, M. (1996) Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal. Chem. 68, 850-858
28. Holmgren, A. (1977) Bovine thioredoxin system. J. Biol. Chem. 252, 4600-4606
29. Luthman, M. and Holmgren, A. (1982) Rat liver thioredoxin and thioredoxin reductase: purification and characterization. Biochemistry 21, 6628-6633
30. Rose, K., Savoy, L-A., Simona, M.G., Offord, R.E., and Wingfield, P. (1988) C-terminal peptide identification by fast atom bombardment mass spectrometry. Biochem. J. 250, 253-259
31. 18O into peptide fragments and its application for protein sequencing by electrospray and matrix-assisted laser desorption/ionization mass spectrometry. Electrophoresis 17, 945-953
32. Zhong, L., Arner, E.S.J., Ljung, J., Aslund, F., and Holmgren, A. (1998) Rat and calf thioredoxin reductase are homologous to glutathione reductase with a carboxyl-terminal elongation containing a conserved catalytically active penultimate selenocysteine residue. J. Biol. Chem. 273, 8581-8591
33. Gorlatov, S.N. and Stadtman, T.C. (1998) Human thioredoxin reductase from HeLa cells: selective alkylation of selenocysteine in the protein inhibits enzyme activity and reduction with NADPH influences affinity to heparin. Proc. Natl. Acad. Sci. USA 95, 8520-8525
34. Rubartelli, A., Bajetto, A., Allavena, G., Wollman, E., and Sitia, R. (1992) Secretion of thioredoxin by normal and neoplastic cells through a leaderless secretory pathway. J. Biol. Chem. 267, 24161-24164
35. Arner, E.S.J., Sarioglu, H., Lottspeich, F., Holmgren, A., and Bock, A. (1999) High-level expression in Escherichia coli of selenocysteine-containing rat thioredoxin reductase utilizing gene fusions with engineered bacterial-type SECIS element and coexpression with selA, selB and selC genes. J. Mol. Biol. 292, 1003-1016