Comparative quantification of IL-1β, IL-10, IL-10r, TNFα and IL-7 mRNA levels in UV-irradiated human skin in vivo

Inflammation Research

Volumn 49, Issue 6, 2000, Pages 290-296

  Brink, N ^a   Szamel, M ^b   Young, A R ^c   Wittern, K P ^a   Bergemann, J ^a  

^a BEIERSDORF AG   (Germany)

^b HANNOVER MEDICAL SCHOOL   (Germany)

^c ST THOMAS HOSPITAL   (United Kingdom)

Author keywords

Cytokine; Irradiation; Receptor; Skin; TaqMan(TM) PCR; UV

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; INTERLEUKIN 10; INTERLEUKIN 10 RECEPTOR; INTERLEUKIN 1BETA; INTERLEUKIN 7; MANGANESE DERIVATIVE; MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA;

ADULT; ARTICLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; FEMALE; GENE EXPRESSION; GENE INDUCTION; HUMAN; HUMAN TISSUE; IMMUNE DEFICIENCY; MALE; RADIATION DOSE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKIN INFLAMMATION; ULTRAVIOLET IRRADIATION;

EID: 0033947148     PISSN: 10233830     EISSN: None     Source Type: Journal    
DOI: 10.1007/PL00000209     Document Type: Article

References (22)

1. [1] Urbach F. Evidence and epidemiology of ultraviolet-induced cancers in man. Natl Cancer Inst Monogr 1978; 50: 5-10.
2. [2] Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res 1996; 6: 986-94.
3. [3] Holland PM, Abramson RD, Watson R, Gelfand DH. Detection of specific polymerase chain reaction product by utilising the 5′-3′ endonuclease activity of Thermus aquaticus DNA polymerase. Proc Natl Acad Sci (USA) 1991; 88: 7276-80.
4. [4] Cruz PD Jr. Effects of UV light on the immune system: answers to five basic questions. American Journal of contact dermatitis 1996; 7: 47-52.
5. [5] Cumberbatch M, Dearman RJ, Kimber I. Interleukin-1β and the stimulation of Langerhans migration: comparison with tumour necrosis factor alpha. Arch Dermatol Res 1997; 289: 277-84.
6. + macrophages that infiltrate human epidermis after in vivo ultraviolet exposure potently produce IL-10 and represent the major secretory source of epidermal IL-10 protein. J Immunol 1994; 153: 5256-64.
7. [7] Beissert S, Hosoi J, Kuhn R, Rajewsky K, Muller W, Granstein RD. Impaired immunosuppressive response to ultraviolet radiation in interleukin-10-deficient mice. J Invest Dermatol 1996; 107: 553-7.
8. [8] Matsue H, Bergstresser PR, Takashima A. Keratinocyte-derived IL-7 serves as a growth factor for dendritic epidermal T cells in mice. J Immunol 1993; 151: 6012-9.
9. [9] Takashima A, Ariizumi K, Bergstresser PR. Mechanism for UVB-induced depletion of dendritic epidermal T cells. J Invest Dermatol 1993; 100: 521-5.
10. [10] Longo N, Berninger NS, Hartly JL. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reaction. Gene 1990; 93: 125-31.
11. [11] Baadgaard O. In vivo ultraviolet irradiation of human skin results in profound perturbation of the immune system: Relevance to ultraviolet-induced skin cancer. Arch Dermatol 1991; 127: 99-109.
12. [12] Cruz PD Jr. Ultraviolet B (UVB)-induced immunosuppression: biological, cellular, and molecular effects. Adv Dermatol 1994; 9: 79-94.
13. [13] Becker-Andre M, Hahlbrock K. Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY). Nucleic Acids Res 1989; 1: 9437-46.
14. [14] Wang AM, Doyle MV, Mark DF. Quantitation of mRNA by the polymerase chain reaction. Proc Natl Acad Sci (USA) 1989; 86: 9717-21.
15. [15] Gilliland G, Perrin S, Blanchard K, Bunn F. Analysis of cytokine mRNA and DNA: detection and quantitation by comparative polymerase chain reaction. Proc Natl Acad Sci (USA) 1990; 87: 2725-9.
16. [16] Klein SC, van Wichen DF, Golverdingen JG, Jacobse KC, Broekhuizen R, de Weger RA. An improved, sensitive, non-radioactive in situ hybridization method for the detection of cytokine mRNAs. APMIS 1995; 103: 345-53.
17. [17] Barr RM, Walker SL, Tsang W, Harrison GI, Ettehadi P, Greaves MW, et al. Suppressed alloantigen presentation, increased TNF-alpha, IL-1, IL-1Ra, IL-10 and modulation of TNF-R in UV-irradiated human skin. J Invest Dermatol 1999; 112: 692-98.
18. [18] Gilchrest BA, Murphy GF, Soter NA. Effect of chronologic ageing and ultraviolet irradiation on Langerhans cells in human epidermis. J Invest Dermatol 1982; 79: 85-8.
19. [19] Strickland I, Rhodes LE, Flanagan BF, Friedmann PS. TNF-alpha and IL-8 are upregulated in the epidermis of normal human skin after UVB exposure: correlation with neutrophil accumulation and E-selectin expression. J Invest Dermatol 1997; 108: 763-8.
20. [20] Groves RW, Allen MH, Ross EL, Barker JN, MacDonald DM. Tumour necrosis factor a is proinflammatory in normal human skin and modulates cutaneous adhesion molecule expression. Br J Dermatol 1995; 132: 345-52.
21. [21] Enk AH, Angeloni VL, Udey MC, Katz SI. An essential role for Langerhans cell-derived IL-1β in the initiation of primary immune responses in skin. J Immunol 1993; 150: 3698-704.
22. [22] Skov L, Hansen H, Allen M, Villadsen L, Norval M, Barker JNWN et al. Contrasting effects of ultraviolet A1 and ultraviolet B exposure on the induction of tumour necrosis factor-alpha in human skin. Br J Dermatol 1997; 138: 216-20.