Pooling cervical swabs and testing by ligase chain reaction are accurate and cost-saving strategies for diagnosis of Chlamydia trachomatis

Journal of Clinical Microbiology

Volumn 38, Issue 7, 2000, Pages 2480-2483

  Kapala, J ^a   Copes, D ^b   Sproston, A ^a   Patel, J ^a   Jang, D ^c   Petrich, A ^c   Mahony, J ^c   Biers, K ^a   Chernesky, M ^c  

^a DYNACARE LABORATORIES   (Canada)

^b Choice in Health Clinic   (Canada)

^c ST JOSEPH S HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CHLAMYDIA TRACHOMATIS; COST CONTROL; DIAGNOSTIC ACCURACY; ENZYME IMMUNOASSAY; LIGASE CHAIN REACTION; NUCLEIC ACID HYBRIDIZATION; PREVALENCE; PRIORITY JOURNAL; URINALYSIS;

CHLAMYDIA TRACHOMATIS; RICKETTSIALES;

EID: 0033943406     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.7.2480-2483.2000     Document Type: Article

References (13)

1. Black, C. M. 1997. Current methods of laboratory diagnosis of Chlamydia trachomatis infections. Clin. Microbiol. Rev. 10:160-184.
2. Chernesky, M., S. Chong, D. Jang, K. Luinstra, M. Faught, and J. Mahony. 1998. Inhibition of amplification of Chlamydia trachomatis plasmid DNA by the ligase chain reaction associated with female urines. Clin. Microbiol. Infect. 4:397-400.
3. Chernesky, M. A., D. Jang, H. Lee, J. D. Burczak, H. Hu, J. Sellors, S. J. Tomazin-Allen, and J. B. Mahony. 1994. Diagnosis of Chlamydia trachomatis infections in men and women by testing first-void urine by ligase chain reaction. J. Clin. Microbiol. 32:2682-2685.
4. Chernesky, M. A., D. Jang, J. Sellors, K. Luinstra, S. Chong, S. Castriciano, and J. B. Mahony. 1997. Urinary inhibitors of polymerase chain reaction and ligase chain reaction and testing of multiple specimens may contribute to lower assay sensitivities for diagnosing Chlamydia trachomatis infected women. Mol. Cell. Probes 11:243-249.
5. Kacena, K. A., S. B. Quinn, M. R. Howell, G. E. Madico, T. C. Quinn, and C. A. Gaydos. 1998. Pooling urine samples for ligase chain reaction screening for genital Chlamydia trachomatis infection in asymptomatic women. J. Clin. Microbiol. 36:481-485.
6. Krepel, J., J. Patel, A. Sproston, F. Hopkins, D. Jang, J. Mahony, and M. Chernesky. 1999. The impact on accuracy and cost of ligase chain reaction testing by pooling urine specimens for the diagnosis of Chlamydia trachomatis infections. Sex. Transm. Dis. 26:504-507.
7. Mahony, J., S. Chong, D. Jang, K. Luinstra, M. Faught, D. Dalby, J. Sellors, and M. Chernesky. 1998. Urine specimens from pregnant and nonpregnant women inhibitory to amplification of Chlamydia trachomatis nucleic acid by PCR, ligase chain reaction, and transcription-mediated amplification: identification of urinary substances associated with inhibition and removal of inhibitory activity. J. Clin. Microbiol. 36:3122-3126.
8. Mahony, J. B., K. E. Luinstra, J. W. Sellors, L. Pickard, S. Chong, D. Jang, and M. A. Chernesky. 1994. Role of confirmatory PCRs in determining performance of chlamydia amplicor PCR with endocervical specimens from women with a low prevalence of infection. J. Clin. Microbiol. 32:2490-2493.
9. Peeling, R. W., B. Toye, P. Jessamine, and I. Gemmill. 1998. Pooling of urine specimens for PCR testing: a cost saving strategy for Chlamydia trachomatis control programmes. Sex. Transm. Infect. 74:66-70.
10. Sherlock, C. H., S. A. Strathdee, T. Le, D. Sutherland, M. V. O'Shaughnessy, and M. T. Schechter. 1995. Use of pooling and out patient laboratory specimens in an anonymous seroprevalence survey of HIV infection in British Columbia, Canada. AIDS 9:945-950.
11. Stamm, W. E. 1999. Chlamydia trachomatis infections: progress and problems. J. Infect. Dis. 179(Suppl. 2):S380-S383.
12. Stary, A., E. Schuh, M. Kerschbaumer, B. Götz, and H. Lee. 1998. Performance of transcription-mediated amplification and ligase chain reaction assays for detection of chlamydial infection in urogenital samples obtained by invasive and noninvasive methods. J. Clin. Microbiol. 36:2666-2670.
13. Wiesenfeld, H. C., R. P. Heine, A. Rideout, I. Macio, F. DiBiasi, and R. L. Sweet. 1996. The vaginal introitus: a novel site for Chlamydia trachomatis testing in women. Am. J. Obstet. Gynecol. 174:1542-1546.