Functional analysis of genes in the rfb locus of Leptospira borgpetersenii serovar Hardjo subtype Hardjobovis

Infection and Immunity

Volumn 68, Issue 7, 2000, Pages 3793-3798

  Bulach, Dieter M ^a   Kalambaheti, Thareerat ^a   De La Peña Moctezuma, Alejandro ^a   Adler, Ben ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

LIPOPOLYSACCHARIDE;

ARTICLE; GENE CLUSTER; GENE CONSTRUCT; GENE FUNCTION; GENE LOCUS; GENE MUTATION; GENETIC ANALYSIS; LEPTOSPIRA; LEPTOSPIROSIS; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SALMONELLA ENTERICA; SEQUENCE HOMOLOGY; VIBRIO CHOLERAE;

ANIMALS; BACTERIAL PROTEINS; CARBOHYDRATE EPIMERASES; CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, BACTERIAL; HUMANS; LEPTOSPIRA; O ANTIGENS; OPEN READING FRAMES; UDPGLUCOSE 4-EPIMERASE;

EID: 0033942976     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.7.3793-3798.2000     Document Type: Article

References (32)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. M. Moore, J. G. Seldman, J. A. Smith, and K. Struhl. 1993. Current protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y.
3. Birnboim, M. J., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
4. Burrows, L. L., D. F. Charter, and J. S. Lam. 1996. Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster. Mol. Microbiol. 22:481-495.
5. Elleman, T. C., and J. E. Peterson. 1987. Expression of multiple types of N-methyl Phe pili in Pseudomonas aeruginosa. Mol. Microbiol. 1:377-80.
6. Faine, S., B. Adler, C. Bolin, and P. Perolat. 1999. Leptospira and Leptospirosis, 2nd ed. MediSci, Melbourne, Australia.
7. Fallarino, A., C. Mavrangelos, U. H. Stroeher, and P. A. Manning. 1997. Identification of additional genes required for O-antigen biosynthesis in Vibrio cholerae O1. J. Bacteriol. 179:2147-2153.
8. Farrelly, H. E., B. Adler, and S. Faine. 1987. Opsonic monoclonal antibodies against lipopolysaccharide antigens of L. interrogans serovar hardjo. J. Med. Microbiol. 23:1-7.
9. Freiberg, C., R. Fellay, A. Bairoch, W. J. Broughton, A. Rosenthal, and X. Perret. 1997. Molecular basis of symbiosis between Rhizobium and legumes. Nature 387:394-401.
10. Hanahan, D. 1983. Studies on the transformation of E. coli with plasmids. J. Mol. Biol. 166:557-580.
11. Hitchcock, P. J., and T. M. Brown. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:269-277.
12. Jiang, X.-M., H. N. Brahmbhatt, N. B. Quigley, and P. R. Reeves. 1987. A low copy number cosmid. Plasmid 18:170-172.
13. Johnson, R. C., J. Walby, R. A. Henry, and N. E. Auran. 1973. Cultivation of parasitic leptospires: effect of pyruvate. Appl. Microbiol. 26:118-119.
14. Jost, B., B. Adler, T. Vinh, and S. Faine. 1986. A monoclonal antibody reacting with a determinant on leptospiral lipopolysaccharide protects guinea pigs against leptospirosis. J. Med. Microbiol. 22:269-275.
15. Kalmbaheti, T., D. M. Bulach, K. Rajakumar, and B. Adler. 1999. Genetic organization of the lipopolysaccharide O-antigen biosynthetic locus of Leptospira borgpetersenii serovar Hardjobovis. Microb. Pathog. 27:105-117.
16. Kiser, K. B., and J. C. Lee. 1998. Staphylococcus aureus cap5O and cap5P genes functionally complement mutations affecting enterobacterial common-antigen biosynthesis in Escherichia coli. J. Bacteriol. 180:403-406.
17. Kolkman, M. A. B., W. Wakarchuk, P. J. M. Nuijten, and B. A. M. van der Zeijst. 1997. Capsular polysaccharide synthesis in Streptococcus pneumoniae serotype 14: molecular analysis of the complete cps locus and identification of genes encoding glycosyltransferases required for the biosynthesis of the tetrasaccharide subunit. Mol. Microbiol. 26:197-208.
18. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
19. Lugtenberg, B., J. Meijers, R. Peters, P. van der Hoek, and L. van Alphen. 1975. Electrophoretic resolution of the 'major outer membrane protein' of Escherichia coli K12 into four bands. FEBS Lett. 58:254-258.
20. Meier-Dieter, U., R. Starman, K. Barr, H. Mayer, and P. D. Rick. 1990. Biosynthesis of enterobacterial common antigen in Escherichia coli. J. Biol. Chem. 265:13490-13497.
21. Moreno, F., R. Rodicio, and P. Herrero. 1981. A new colorimetric assay for UDP-glucose 4-epimerase activity. Cell. Mol. Biol. 27:589-592.
22. Rick, P. D., H. Mayer, B. A. Neumeyer, S. Wolski, and D. Bitter-Suermann. 1985. Biosynthesis of enterobacterial common antigen. J Bacteriol. 162:494-503.
23. Sambrook, J., E. K. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Plainview, N.Y.
24. 10 E. coli transformants/μg. Focus 12:38-40.
25. Sugiyama, T., N. Kido, Y. Arakawa, M. Mori, S. Naito, M. Ohta, and N. Kato. 1990. Rapid small-scale preparation method of cell surface polysaccharides. Microbiol. Immunol. 34:635-641.
26. Tsai, C.-M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:115-119.
27. van Kranenburg, R., I. I. van Swam, J. D. Marugg, M. Kleerebezem, and W. M. de Vos. 1999. Exopolysaccharide biosynthesis in Lactococcus lactis NIZO B40: functional analysis of the glycosyltransferase genes involved in synthesis of the polysaccharide backbone. J. Bacteriol. 181:338-340.
28. Wang, L., D. Liu, and P. R. Reeves. 1996. C-terminal half of Salmonella enterica WbaP (RfbP) is the galactosyl-1-phosphate transferase domain catalyzing the first step of O-antigen synthesis. J. Bacteriol. 178:2598-604.
29. Wang, R. F., and S. R. Kushner. 1991. Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100:195-199.
30. West, S. E. H., H. P. Schweizer, C. Dall, A. K. Sample, and A. L. J. Runyen-Janecky. 1994. Construction of improved Escherichia-Pseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa. Gene 148:81-86.
31. Westphal, O., and K. Jann. 1965. Bacterial lipopolysaccharides; extraction with phenol-water and further applications of the procedure. Methods Carbohydr. Chem. 5:83-91.
32. Wilkinson, R. G., P. Gemski, and B. A. Stocker. 1972. Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. J. Gen. Microbiol. 70:527-554.