Capsular polysaccharide synthesis in Streptococcus pneumoniae serotype 14: Molecular analysis of the complete cps locus and identification of genes encoding glycosyltransferases required for the biosynthesis of the tetrasaccharide subunit

Molecular Microbiology

Volumn 26, Issue 1, 1997, Pages 197-208

  Kolkman, Marc A B ^a   Wakarchuk, Warren ^b   Nuijten, Piet J M ^a,c   Van Der Zeijst, Bernard A M ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^c INTERVET INTERNATIONAL BV   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOSYLTRANSFERASE; POLYSACCHARIDE;

ARTICLE; BACTERIAL MEMBRANE; BIOSYNTHESIS; CLONING; CONTROLLED STUDY; GENE LOCUS; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; SEROTYPE; STREPTOCOCCUS PNEUMONIAE;

EID: 0030867784     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.5791940.x     Document Type: Article

References (46)

1. Alloing, G., de Philip, P., and Claverys, J.-P. (1994) Three highly homologous membrane-bound lipoproteins participate in oligopeptide transport by the Ami system of the Gram-positive Streptococcus pneumoniae. J Mol Biol 241: 44-58.
2. Arrecubieta, C., García, E., and López, R. (1995) Sequence and transcriptional analysis of a DNA region involved in the production of capsular polysaccharide in Streptococcus pneumoniae type 3. Gene 167: 1-7.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Smith, J.A., Seidman, J.G., and Struhl, K. (1987) Current Protocols in Molecular Biology, New York: John Wiley & Sons.
4. Avery, O.T., MacLeod, C.M., and McCarty, M. (1944) Studies on the chemical nature of the substance inducing transformation of pneumococcal types. Induction of transformation by a desoxyribonucleic acid fraction isolated from pneumococcus type III. J Exp Med 79: 137-158.
5. Bugert, P., and Geider, K. (1995) Molecular analysis of the ams operon required for exopolysaccharide synthesis of Erwinia amylovora. Mol Microbiol 15: 917-933.
6. Cross, A.S. (1990) The biological significance of bacterial encapsulation. Curr Topics Microbiol Immunol 150: 87-95.
7. Dillard, J.P., Vandersea, M.W., and Yother, J. (1995) Characterization of the cassette containing genes for type 3 capsular polysaccharide biosynthesis in Streptococcus pneumoniae. J Exp Med 181: 973-983.
8. Fischer, W., Behr, T., Hartmann, R., Peter-Katalinic, J., and Egge, H. (1993) Teichoic acid and lipoteichoic acid of Streptococcus pneumoniae possess identical chain structures. A reinvestigation of teichoic acid (C polysaccharide). Eur J Biochem 215: 851-857.
9. García, E., and Löpez, R. (1997) Molecular biology of the capsular genes of Streptococcus pneumoniae. FEMS Microbiol Lett 149: 1-10.
10. Gawron-Burke, C., and Clewell, D.B. (1984) Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning genes from Gram-positive bacteria. J Bacteriol 159: 214-221.
11. Glucksmann, M.A., Reuber, T.L., and Walker, G.G. (1993) Family of glycosyltransferases needed for the synthesis of succinoglycan by Rhizobium meliloti. J Bacteriol 175: 7033-7044.
12. Griffith, F. (1928) The significance of pneumococcal types. J Hyg 27: 113-159.
13. Guidolin, A., Morona, J.K., Morona, R., Hansman, D., and Paton, J.C. (1994) Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F. Infect Immun 62: 5384-5396.
14. Henrichsen, J. (1995) Six newly recognized types of Streptococcus pneumoniae. J Clin Microbiol 33: 2759-2762.
15. Jennings, H.J. (1990) Capsular polysaccharides as vaccine candidates. Curr Topics Microbiol Immunol 150: 97-128.
16. Jennings, M.P., Hood, D.W., Peak, I.R.A., Virji, M., and Moxon, E.R. (1995) Molecular analysis of a locus for the biosynthesis and phase-variable expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis. Mol Microbiol 18: 729-740.
17. Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., Kimura, T., Hosouchi, T., Matsuno, A., Muraki, A., Nazaki, N., Naruo, K., Okumura, S., Shimpo, S., Takeuchi, C., Wada, T., Watanabe, A., Yamada, M., Yasuda, M., and Tabata, S. (1996) Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res 3: 109-136.
18. Klein, P., Kanehisa, M., and DeLisi, C. (1985) The detection and classification of membrane spanning proteins. Biochim Biophys Acta 815: 468-476.
19. Klena, J.D., and Schnaitman, C.A. (1993) Function of the rfb gene cluster and rfe gene in the synthesis of O antigen by Shigella dysenteriae 1. Mol Microbiol 9: 393-402.
20. Kolkman, M.A.B., Morrison, D.A., van der Zeijst, B.A.M., and Nuijten, P.J.M. (1996) The capsule polysaccharide synthesis locus of Streptococcus pneumoniae serotype 14: identification of the glycosyltransferase gene cps 14E. J Bacteriol 178: 3736-3741.
21. Kolkman, M.A.B., van der Zeijst, B.A.W., and Nuijten, P.J.M. (1997) Functional analysis of glycosyltransferases encoded by the capsular polysaccharide locus of Streptococcus -pneumoniae serotype 14. J Biol Chem 272: 19502-19508.
22. Lazarevic, V., Margot, P., Soldo, B., and Karamata, D. (1992) Sequencing and analysis of the Bacillus subtilis lytRABC divergon: a regulatory unit encompassing the structural genes of the N-acetylmuramoyl-L-alanine amidase and its modifier. J Gen Microbiol 138: 1949-61.
23. Lin, W.S., Cunneen, T., and Lee, C.Y. (1994) Sequence analysis and molecular characterization of genes required for the biosynthesis of type 1 capsular polysaccharide in Staphylococcus aureus. J Bacteriol 176: 7005-7016.
24. Liu, D., Cole, R.A., and Reeves, P.R. (1996) An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase. J Bacteriol 178: 2102-2107.
25. Martin, B., Humbert, O., Camara, M., Guenzi, E., Walker, J., Mitchell, T., Andrew, P., Prudhomme, M., Alloing, G., Hakenbeck, R., Morrison, D.A., Boulnois, G.J., and Claverys, J.P. (1992) A highly conserved repeated DNA element located in the chromosome of Streptococcus pneumoniae. Nucleic Acids Res 20: 3479-3483.
26. Morona, J.K., Guidolin, A., Morona, R., Hansman, D., and Paton, J.C. (1994) Isolation, characterization, and nucleotide sequence of IS 1202, an insertion sequence of Streptococcus pneumoniae. J Bacteriol 176: 4437-4443.
27. Morona, J.K., Morona, R., and Paton, J.C. (1997) Characterization of the locus encoding the Streptococcus pneumoniae type 19F capsular polysaccharide biosynthetic pathway. Mol Microbiol 23: 751-763.
28. Moxon, E.R., and Kroll, J.S. (1990) The role of bacterial polysaccharide capsules as virulence factors. Curr Topics Microbiol Immunol 150: 65-85.
29. Muñoz, R., Mollerach, M., López, R., and García, E. (1997) Molecular organization of the genes required for the synthesis of type 1 capsular polysaccharide of Streptococcus pneumoniae: formation of binary encapsulated pneumococci and identification of cryptic dTDP-rhamnose biosynthesis genes. Mol Microbiol 25: 79-92.
30. Pooley, H.M., Abellan, F.-X., and Karamata, D. (1992) CDP-glycerol:poly(glycerophosphate) glycerophosphotransferases, which is involved in the synthesis of the major wall teichoic acid in Bacillus subtilis 168, is encoded by tagF (rodC). J Bacteriol 174: 646-649.
31. Pozzi, G., Masala, L., Iannelli, F., Manganelli, R., Håvarstein, L.S., Piccoli, L., Simon, D., and Morrison, D.A. (1996) Competence for genetic transformation in encapsulated strains of Streptococcus pneumoniae: two allelic variants of the peptide pheromone. J Bacteriol 178: 6087-6090.
32. Reuber, T.L., and Walker, G.C. (1993) Biosynthesis of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti. Cell 74: 269-280.
33. Roberts, I.S. (1996) The biochemistry and genetics of capsular polysaccharide production in bacteria. Annu Rev Microbiol 50: 285-315.
34. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
35. Saxena, I.M., Brown, Jr, R.M., Fevre, M., Geremia, R.A., and Henrissat, B. (1995) Multidomain architecture of β-glycosyl transferases: implications for mechanism of action. J Bacteriol 177: 1419-1424.
36. Selbitschka, W., Arnol, W., Jording, D., Kosier, B., Toro, N., and Pühler, A. (1995) The insertion sequence element ISRm2011-2 belongs to the IS630-Tc1 family of transposable elements and is abundant in Rhizobium meliloti. Gene 163: 59-64.
37. Siber, G.R. (1994) Pneumococcal disease: prospects for a new generation of vaccines. Science 265: 1385-1387.
38. Skurnik, M., Venho, R., Toivanen, P., and Al-Hendy, A. (1995) A novel locus of Yersinia enterocolitica serotype O:3 involved in lipopolysaccharide outer core biosynthesis. Mol Microbiol 17: 575-594.
39. Stingele, F., Neeser, J.-R., and Mollet, B. (1996) Identification and characterization of the eps (exopolysaccharide) gene cluster from Streptococcus thermophilus Sfi6. J Bacteriol 178: 1680-1690.
40. Van Dam, J.E.G., Fleer, A., and Snippe, H. (1990) Immunogenicity and immunochemistry of Streptococcus pneumoniae capsular polysaccharides. Antonie van Leeuwenhoek 58: 1-47.
41. Van Eldere, J., Brophy, L., Loynds, B., Celis, P., Hancock, I., Carman, S., Kroll, J.C., and Moxon, E.R. (1995) Region II of the Haemophilus influenzae type b capsulation locus is involved in serotype-specific polysaccharide synthesis. Mol Microbiol 15: 107-118.
42. Wakarchuk, W., Martin, A., Jennings, M.P., Moxon, E.R., and Richards, J.C. (1996) Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis. J Biol Chem 271: 19166-19173.
43. Watson, D.A., and Musher, D.M. (1990) Interruption of capsule production in Streptococcus pneumoniae serotype 3 by insertion of transposon Tn976. Infect Immun 58: 3135-3138.
44. Yao, Z., and Valvano, M.A. (1994) Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a. J Bacteriol 176: 4133-4143.
45. Zhang, L., Radziejewska-Lebrecht, J., Krajewska-Pietrasik, D., Toivanen, P., and Skurnik, M. (1997) Molecular and chemical characterization of the lipopolysaccharide O-antigen and its role in the virulence of Yersinia enterocolitica serotype O:8. Mol Microbiol 23: 63-76.
46. Zhou, L., Hui, F.M., and Morrison, D.A. (1995) Characterization of IS 1167, a new insertion sequence in Streptococcus pneumoniae. Plasmid 33: 127-138.