Recycling of importin α from the nucleus is suppressed by loss of RCC1 function in living mammalian cells

Cell Structure and Function

Volumn 25, Issue 2, 2000, Pages 115-123

  Tachibana, Taro ^a,b   Hieda, Miki ^a   Miyamoto, Yoichi ^a   Kose, Shingo ^a   Imamoto, Naoko ^a   Yoneda, Yoshihiro ^a,b  

^a OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Importin ; Nuclear protein transport; Ran; RCC1

Indexed keywords

GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; KARYOPHERIN; VIRUS T ANTIGEN;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CELL STRAIN BHK; CONTROLLED STUDY; CYTOPLASM; GENE FUNCTION; GENE LOSS; GENE REPRESSION; HETEROKARYON; IMMUNOCYTOCHEMISTRY; MAMMAL CELL; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN TRANSPORT; RECYCLING; SIMIAN VIRUS 40;

ANIMALS; CELL CYCLE PROTEINS; CELL LINE; CELL NUCLEUS; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; COCULTURE TECHNIQUES; CYTOPLASM; DNA-BINDING PROTEINS; FLUORESCENT DYES; GUANINE NUCLEOTIDE EXCHANGE FACTORS; KARYOPHERINS; MICE; MICROINJECTIONS; NUCLEAR LOCALIZATION SIGNALS; NUCLEAR PORE; NUCLEAR PROTEINS; PROTEIN BINDING; PROTEINS; RAN GTP-BINDING PROTEIN; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

ANIMALIA; MAMMALIA; SIMIAE; SIMIAN VIRUS; SIMIAN VIRUS 40;

EID: 0033939104     PISSN: 03867196     EISSN: None     Source Type: Journal    
DOI: 10.1247/csf.25.115     Document Type: Article

References (39)

1. Adam, E.J. and Adam, S.A. 1994. Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope. J. Cell Biol. 125: 547-555.
2. Bischoff, F.R. and Görlich, D. 1997. RanBP1 is crucial for the release of RanGTP from importin β-related nuclear transport factors. FEBS Lett., 419: 249-254.
3. Bischoff, F.R., Klebe, C., Kretschmer, J., Wittinghofer, A., and Ponstingl, H. 1994. RanGAP1 induces GTPase activity of nuclear Ras-related Ran. Proc. Natl. Accid. Sci. USA, 91: 2587-2591.
4. Bischoff, F.R., Krebber, H., Kempf, T., Hermes, I., and Ponstingl, H. 1995. Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport. Proc. Natl. Acad. Sci.USA, 92: 1749-1753.
5. Bischoff, F.R. and Ponstingl, H. 1991a. Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1. Nature, 354: 80-82.
6. Bischoff, F.R. and Ponstingl, H. 1991b. Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Proc. Natl. Acad Sci. USA, 88: 10830-10834.
7. Enenkel, C., Blobel, G., and Rexach, M. 1995. Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes. J. Biol. Chem., 270: 16499-16502.
8. Görlich, D., Kostka, S., Kraft, R., Dingwall, C., Laskey, R.A., Hartmann, E., and Prehn, S. 1995. Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope. Curr. Biol., 5: 383-392.
9. Görlich, D., Pante, N., Kutay, U., Aebi, U., and Bischoff, F.R. 1996. Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J., 15: 5584-5594.
10. Hieda, M., Tachibana, T., Yokoya, F., Kose, S., Imamoto, N., and Yoneda, Y. 1999. A monoclonal antibody to the COOH-terminal acidic portion of Ran inhibits both the recycling of Ran and nuclear protein import in living cells. J. Cell Biol., 144:645-655.
11. Imamoto, N., Shimamoto, T., Takao, T., Tachibana, T., Kose, S., Matsubae, M., Sekimoto, T., Shimonishi, Y., and Yoneda, Y. 1995a. In vivo evidence for involvement of a 58 kDa component of nuclear pore- targeting complex in nuclear protein import. EMBO J., 14: 3617-3626.
12. Imamoto, N., Tachibana, T, Matsubae, M., and Yoneda., Y. 1995b. A karyophilic protein forms a stable complex with cytoplasmic components prior to nuclear pore binding. J. Biol. Chem., 270: 8559-8565.
13. Izaurralde, E., Kutay, U., von Kobbe, C., Mattaj, I.W., and Görlich, D. 1997. The asymmetric distribution of the constituents, of the Ran system is essential for transport into and out of the nucleus. EMBO J., 16: 6535-6547.
14. Kamei, Y., Yuba, S., Nakayama, T., and Yoneda, Y. 1999. Three distinct classes of the α-subunit of the nuclear pore-targeting complex (importin-α) are differentially expressed in adult mouse tissues. J. Histochem. Cytochem., 47: 363-372.
15. Koepp, D.M., Wong, D.H., Corbett, A.H., and Silver, P.A. 1996. Dynamic localization of the nuclear import receptor and its interactions with transport factors. J. Cell Biol., 133: 1163-1176.
16. Köhler, M., Ansieau, S., Prehn, S., Leutz, A., Haller, H., and Hartmann, E. 1997. Cloning of two novel human importin-α subunits and analysis of the expression pattern of the importin-α protein family. FEBS Lett., 417: 104-108.
17. Kose, S., Imamoto, N., Tachibana, T., Yoshida, M., and Yoneda, Y. 1999. β-Subunit of nuclear pore-targeting complex (importin-β) can be exported from the nucleus in a Ran-independent manner. J. Biol. Chem., 274: 3946-3952.
18. Kutay, U., Bischoff, F.R., Kostka, S., Kraft, R., and Görlich, D. 1997. Export of importin α from the nucleus is mediated by a specific nuclear transport factor. Cell 90: 1061-1071.
19. Mahajan, R., Gerace, L., and Melchior, F. 1998. Molecular characterization of the SUMO-1 modification of RanGAPI and its role in nuclear envelope association. J. Cell Biol., 140:259-270.
20. Mattaj, I.W. and Englmeier, L. 1998. Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem., 67: 265-306.
21. Matunis, M.J., Wu, J., and Blobel, G. 1998. SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAPI, to the nuclear pore complex. J. Cell Biol. 140: 499-509.
22. Miyamoto, Y., Imamoto, N., Sekimoto, T., Tachibana, T., Seki, S., Tada, Enomoto, T., and Yoneda, Y. 1997. Differential modes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors. J. Biol. Chem., 272: 26375-26381.
23. Moore, M.S. and Blobel, G 1994. Purification of a Ran-interacting protein that is required for protein import into the nucleus. Proc. Natl. Acad. Sci. USA, 91: 10212-10216.
24. Moroianu, J., Blobel, G., and Radu, A. 1995. Previously identified protein of uncertain function is karyopherin α and together with karyopherin β docks import substrate at nuclear pore complexes. Proc. Natl. Acad. Sci. USA, 92: 2008-2011.
25. Moroianu, J., Blobel, G, and Radu, A. 1996. Nuclear protein import: RanGTP dissociates the karyopherin α/β heterodimer by displacing α from an overlapping binding site on β. Proc. Natl. Acad Sci. USA, 93: 7059-7062.
26. Nakielny, S., Siomi, M.C., Siomi, H., Michael, W.M., Pollard, V., and Dreyfuss, G. 1996. Transportin: nuclear transport receptor of a novel nuclear protein import pathway. Exp. Cell Res., 229: 261-266.
27. Paschal, B.M. and Gerace, L. 1995. Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J. Cell Biol., 129: 925-937.
28. Pollard, V.W., Michael, W.M., Nakielny, S., Siomi, M.C., Wang, F., and Dreyfuss, G. 1996. A novel receptor-mediated nuclear protein import pathway. Cell. 86: 985-994.
29. Rexach, M. and Blobel, G. 1995. Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell, 83: 63-692.
30. Ribbeck, K., Lipowsky, G, Kent, H.M., Stewart, M., and Görlich, D. 1998. NTF2 mediates nuclear import of Ran. EMBO J., 17: 6587-6598.
31. Sekimoto, T., Imamoto, N., Nakajima, K., Hirano, T., and Yoneda, Y. 1997. Extracellular signal-dependent nuclear import of Stall is mediated by nuclear pore-targeting complex formation with NPI-1, but not Rchl. EMBO J., 16: 7067-7077.
32. Smith, A., Brownawell, A., and Macara, I.G 1998. Nuclear import of Ran is mediated by the transport factor NTF2. Curr. Biol., 8: 1403-1406.
33. Tachibana, T., Imamoto, N., Seino, H., Nishimoto, T., and Yoneda, Y. 1994. Loss of RCC1 leads to suppression of nuclear protein import in living cells. J. Biol. Chem., 269: 24542-24545.
34. Tsuji, L., Takumi, T., Imamoto, N., and Yoneda, Y. 1997. Identification of novel homologues of mouse importin α, the α subunit of the nuclear pore-targeting complex, and their tissue-specific expression. FEBS Lett., 416: 30-34.
35. Uchida, S., Sekiguchi, T., Nishitani, H., Miyauchi, K., Ohtsubo, M., and Nishimoto, T. 1990. Premature chromosome condensation is induced by a point mutation in the hamster RCC1 gene. Mol. Cell. Biol., 10: 577-584.
36. Yamada, M., Tachibana, T, Imamoto, N., and Yoneda. Y., 1998. Nuclear transport factor plO/NTF2 functions as a Ran-GDP dissociation inhibitor (Ran-GDI). Curr. Biol, 8: 1339-1342.
37. Yano, R., Oakes, M., Yamaghishi, M., Dodd, J.A., and Nomura, M., 1992. Cloning and characterization of SRP1, a suppressor of temperature-sensitive RNA polymerase I mutations, in Saccharomyces cerevisiae. Mol. Cell.Biol., 12: 5640-5651.
38. Yoneda, Y. 1997. How proteins are transported from cytoplasm to the nucleus. J. Biochem. (Tokyo)., 121: 811-817.
39. Yoneda, Y., Arioka, T., Imamoto-Sonobe, N., Sugawa, H., Shimonishi, Y., and Uchida, T. 1987. Synthetic peptides containing a region of SV40 large T-antigen involved in nuclear localization direct the transport of proteins into the nucleus. Exp. Cell Res., 170: 439-452.