Rapid screening for putative exported proteins from Staphylococcus aureus using alkaline phosphatase as a reporter molecule

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 15, Issue 1, 2000, Pages 11-20

  Williams, R J ^a,b   Ward, J M ^b   Henderson, B ^a   Wilson, M ^a   Nair, S P ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

PhoA fusion proteins; Secreted proteins; Staphylococcus aureus

Indexed keywords

ALKALINE PHOSPHATASE; VIRULENCE FACTOR;

AMINO ACID SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL VIRULENCE; GENE ACTIVITY; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROTEIN ANALYSIS; REPORTER GENE; SEQUENCE HOMOLOGY; STAPHYLOCOCCUS AUREUS;

EID: 0033938223     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:15:1:11     Document Type: Article

References (30)

1. 1. Archer, G.L. and Crossley, K.B. (1997) The Staphylococci in Human Disease, Churchill. Livingstone, NY.
2. 2. Hiramatsu K., Hanaki, H., Ino, T., Yabuta, K., Oguri, T., and Tenover, F.C. (1997) Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. J. Antimicrob. Chemother. 40, 135-136.
3. 3. Bohach, G.A., Dinges, M.M., Mitchell, D.T., Ohlendorf, D.H., and Schlievert, P.M. (1997) Exotoxins, in The Staphylococci in Human Disease (Crossley, K.B. and Archer, G.L., eds.), Churchill Livingstone, NY. pp. 83-111.
4. 4. Patti, J.M., Jonsson, H., Guss, B., Switalski, L.M., Wiberg, K., Lindberg, M., and Hook, M. (1992) Molecular characterization and expression of a gene encoding a Staphylococcus aureus collagen adhesin. J. Biol. Chem. 267, 4766-4772.
5. 5. McDevitt, D., Francois, P., Vaudaux, P., and Foster, T.J. (1994). Molecular characterization of the dumping factor (fibrinogen receptor) of Staphylococcus aureus. Mol. Microbiol. 11, 237-248.
6. 6. Nucifora, G., Chu, L., Misra, T.K., and Silver, S. (1989) Cadmium resistance from Staphylococcus aureus plasmid pI258 cadA gene results from a cadmium-efflux ATPase. Proc. Natl. Acad. Sci. USA 86, 3544-3548.
7. 7. Nair, S., Song, Y., Meghji, S., Reddi, K., Harris, M., Ross, A., Pool, S., Wilson, M., and Henderson, B. (1995) Surface-associated proteins from Staphylococcus aureus demonstrate potent bone resorbing activity. J. Bone and Miner. Res. 10, 726-734.
8. 8. Meghji, S., Crean, S.J., Hill, P.A., Sheikh, M., Nair, S.P., Heron, K., Henderson, B., Mawer, E.B., and Harris, M. (1998) Surface-associated protein from Staphylococcus aureus stimulates osteoclastogenesis: Possible role in S. aureus-induced bone pathology. Br. J. Rheumatol. 37,1095-1101.
9. 9. Arora, M., Shah, N., Meghji, S., Henderson, B., Harris, M., Nair, S., Wilson, M., Gray, C.M., Jones, S.J., and Boyde, A. (1998) Effect of Staphylococcus aureus extracellular proteinaceous fraction in an isolated osteoclastic resorption assay. J. Bone Miner. Metab. 16, 158-161.
10. 10. Manoil, C., Makalanos, J.J., and Beckwith, J. (1990) Alkaline phosphatase fusions: sensors of subcellular location. J. Bacteriol. 172, 515-518.
11. 11. Calamia, J. and Manoil, C. (1990) Lac permease of Escherichia coli: Topology and sequence elements promoting membrane insertion. Proc. Natl. Acad. Sci. USA 87, 4937-4941.
12. 12. Pearce, B.J., Yin, Y.B., and Masure, H.R. (1993) Genetic identification of exported proteins in Streptococcus pneumoniae. Mol. Microbiol. 9, 1037-1050.
13. 13. Bergdoll, M.S., Borja, C.R., Robbins, R., and Weiss, K.F. (1971) Identification of enterotoxin E. Infect. Immun. 4, 593-595.
14. 14. Manoil, C. and Beckwith, J. (1985) TnphoA: A transposon probe for protein export signals. Proc. Natl. Acad. Sci. USA 82, 8129-8133.
15. 15. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
16. 16. Roe, B.A., Yudong Qian, Dorman, A., Najar, F.Z., Clifton, S., and Iandolo, J. Staphylococcus aureus genome sequencing project. www.genome.ou.edu/ staph.htlm. Funded by NIH and Merck Research Foundation.
17. 17. Hofmann, K. and Stoffel, W. (1993) TMbase-A database of membrane spanning proteins segments. Biol. Chem. Hoppe-Seyler 347, 166.
18. 18. Nielsen, H., Engelbrecht, J., Brunak, S., and von Heijne, G. (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10, 1-6.
19. 19. Gravet, A., Colin, D.A., Keller, D., Giradot, R., Monteil, H., and Prevost, G. (1998) Characterisation of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family. FEBS Lett. 436, 202-208.
20. 20. Farrell, A.M., Taylor, D., and Holland, K.T. (1995) Cloning, nucleotide sequence determination and expression of the Staphylococcus aureus hyaluronate lyase gene. FEMS Microbiol. Letts. 130, 81-85.
21. 21. Wada, A. and Watanabe, H. (1998) Penicillin-binding protein 1 of Staphylococcus aureus is essential for growth. J. Bacteriol. 180, 2759-2765.
22. 22. Ramadurai, L. and Jayaswal, R.K. (1997) Molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. J. Bacteriol. 179, 3625-3631.
23. 23. Lowe, A.M., Beattie, D.T. and Deresiewicz, R.L. (1998) Identification of novel staphylococcal virulence genes by in vivo expression technology. Mol. Microbiol. 27, 967-976.
24. 24. Ni Eidhin, D., Perkins, S., Francois, P., Vaudaux, P., Hook, M. and Foster, T.J. (1998) Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus. Mol. Microbiol. 30, 245-57
25. 25. Patti, J.M., Allen, B.L., McGavin, M.J., and Hook, M. (1994) MSCRAMM-mediated adherence of microorganisms to host tissues. Annu. Rev. Microbiol. 48, 585-617.
26. 26. Schwan, W.R., Coulter, S.N., Ng, E.Y.W., Langhorne, M.H., Ritchie, H.D., Brody, L.L., Westbrock-Wadman, S., Bayer, A.S., Folger, K.R., and Stover, C.K. (1998) Identification and characterization of the PutP proline permease that contributes to in vivo survival of Staphylococcus aureus in animal models. Infect. Immun. 66, 567-572.
27. 27. Yousif, Y., Schiltz, E., Okada, K., Batsford, S., and Vogt, A. (1994) Staphylococcal neutral phosphatase. A highly cationic molecule with binding properties for immunoglobulin. APMIS 102, 891-900.
28. 28. Roberson, J.R., Fox, L.K., Hancock, D.D., and Besser, T.E. (1992) Evaluation of methods for differentiation of coagulase-positive staphylococci. J. Clin. Micro. 30, 3217-3219.
29. 29. Jackson, M.P. and Iandolo, J.J. (1986) Cloning and expression of the exfoliative toxin B gene from Staphylococcus aureus. J. Bacteriol. 166, 574-580.
30. 30. Ranelli, D.M., Jones, C.L., Johns, M.B., Mussey, G.J., and Khan, S.A. (1985) Molecular cloning of staphylococcal enterotoxin B gene in Escherichia coli and Staphylococcus aureus. Proc. Natl. Acad. Sci. USA 82, 5850-5854.