Mechanism of metronidazole resistance in Helicobacter pylori: Comparison of the rdxA gene sequences in 30 strains

Antimicrobial Agents and Chemotherapy

Volumn 44, Issue 8, 2000, Pages 2207-2210

  Solcà, Nadia Maggi ^a   Bernasconi, Marco Valerio ^a   Piffaretti, Jean Claude ^a  

^a Istituto Batteriosierologico Cantonale   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

METRONIDAZOLE;

AMINO ACID SUBSTITUTION; ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; GENE SEQUENCE; HELICOBACTER PYLORI; MISSENSE MUTATION; NONHUMAN; NONSENSE MUTATION; NUCLEOTIDE SEQUENCE; PHYLOGENY; POINT MUTATION; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; DRUG RESISTANCE, MICROBIAL; HELICOBACTER PYLORI; HUMANS; MEMBRANE PROTEINS; METRONIDAZOLE; MOLECULAR SEQUENCE DATA; PHYLOGENY; POINT MUTATION; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0033928525     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.44.8.2207-2210.2000     Document Type: Article

References (13)

1. Achtman, M., T. Azuma, D. E. Berg, Y. Ito, G. Morelli, Z. J. Pan, S. Suerbaum, S. A. Thompson, A. Van der Ende, and L. J. Van Doorn. 1999. Recombination and clonal groupings within Helicobacter pylori from different geographical regions. Mol. Microbiol. 32:459-470.
2. Alarcon, T., D. Domingo, and M. Lopez-Brea. 1999. Antibiotic resistance problems with Helicobacter pylori. Int. J. Antimicroh. Agents 12:19-26.
3. European Helicobacter pylori Study Group. 1997. Current European concepts in the management of Helicobacter pylori infection. The Maastricht Consensus Report. Gut 41:8-13.
4. European Study Group on Antibiotic Susceptibility of Helicobacter pylori. 1992. Results of a multicentre European survey in 1991 of metronidazole resistance in Helicobacter pylori. Eur. J. Clin. Microbiol. Infect. Dis. 11:777-781.
5. Goodwin, A., D. Kersulyte, G. Sisson, S. J. Veldhuyzen van Zanten, D. E. Berg, and P. S. Hoffman. 1998. Metronidazole resistance in Helicobacter pylori is due to null mutations in a gene (rdxA) that encodes an oxygen-insensitive NADPH nitroreductase. Mol. Microbiol. 28:383-393.
6. Hoffman, P. S., A. Goodwin, J. Johnsen, K. Magee, and S. J. Veldhuyzen van Zanten. 1996. Metabolic activities of metronidazole-sensitive and -resistant strains of Helicobacter pylori: repression of pyruvate oxidoreductase and expression of isocitrate lyase activity correlate with resistance. J. Bacteriol. 178:4822-4829.
7. Jenks, P. J., R. L. Ferrero, and A. Labigne. 1999. The role of the rdxA gene in the evolution of metronidazole resistance in Helicobacter pylori. J. Antimicrob. Chemother. 43:753-758.
8. Kumar, S., K. Tamura, and M. Nei. 1993. Molecular evolutionary genetics analysis, version 1.01. The Pennsylvania State University, University Park, Pa.
9. Ling, T. K., A. F. Cheng, J. J. Sung, P. Y. Yiu, and S. S. Chung. 1996. An increase in Helicobacter pylori strains resistant to metronidazole: a five-year study. Helicobacter 1:57-61.
10. Maggi-Solcà, N., C. Valsangiacomo, and J.-C. Piffaretti. Prevalence of Helicobacter pylori resistant strains in the Southern part of Switzerland. Clin. Microbiol. Infect. 6:38-40.
11. Mégraud, F., N. Lehn, T. Lind, E. Bayerdörffer, C. O'Morain, R. Spiller, P. Unge, S. Veldhuyzen van Zanten, M. Wrangstadh, and C. F. Burman. 1999. Antimicrobial susceptibility testing of Helicobacter pylori in a large multicenter trial: the MACH 2 study. Antimicrob. Agents Chemother. 43:2747-2752.
12. National Committee for Clinical Laboratory Standards. 2000. Performance standards for antimicrobial disk susceptibility tests, 7th ed. M100-S10. National Committee for Clinical Laboratory Standards, Wayne, Pa.
13. Rautelin, H., K. Seppala, O. V. Renkonen, U. Vainio, and T. U. Kosunen. 1992. Role of metronidazole resistance in therapy of Helicobacter pylori infections. Antimicrob. Agents Chemother. 36:163-166.