Genetic diversity in populations of Uncinula necator: Comparison of RFLP- and PCR-based approaches

Mycological Research

Volumn 104, Issue 1, 2000, Pages 44-52

  Stummer, B E ^a   Zanker, T ^a   Scott, E S ^a   Whisson, D L ^a,b  

^a UNIVERSITY OF ADELAIDE   (Australia)

^b SOUTH AUSTRALIAN RESEARCH AND DEVELOPMENT INSTITUTE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CLONE; DNA PROBE; GENETIC DIVERSITY; HAPLOTYPE; INTRON; MATING TYPE; POLYMERASE CHAIN REACTION; PRIMER DNA; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ERYSIPHALES; FUNGI; NECATOR; UNCINULA NECATOR; VITIS;

EID: 0033922258     PISSN: 09537562     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0953756299001070     Document Type: Article

References (32)

1. Brown, J. K. M., O'Dell, M., Simpson, C. G. & Wolfe, M. S. (1990). The use of DNA polymorphisms to test hypotheses about a population of Erysiphe graminis f. sp. hordei. Plant Pathology 39, 391-401.
2. Brown, J. K. M. & Simpson, C. G. (1994). Genetic analysis of DNA fingerprints and virulences in Erysiphe graminis f. sp. hordei. Current Genetics 26, 172-178.
3. Christiansen, S. K. & Giese, H. (1990). Genetic analysis of the obligate parasitic barley powdery mildew fungus based on RFLP and virulence loci. Theoretical and Applied Genetics 79, 705-712.
4. Délye, C., Corio-Costet, M.-F. & Laigret, F. (1995). A RAPD assay for strain typing of the biotrophic grape powdery mildew fungus Uncinula necator using DNA extracted from the mycelium. Experimental Mycology 19, 234-237.
5. Délye, C., Laigret, F. & Corio-Costet, M.-F. (1997). RAPD analysis provides insight into the biology and epidemiology of Uncinula necator. Phytopathology 87, 670-677.
6. Doyle, J. J. & Doyle, J. L. (1980). Isolation of plant DNA from fresh tissue. Focus 12, 13-15.
7. Edel, V., Steinberg, C., Avelange, I., Laguerre, G. & Alabouvette, C. (1995). Comparison of three molecular methods for the characterization of Fusarium oxysporum strains. Phytopathology 85, 579-585.
8. Erickson, E. O. & Wilcox, W. F. (1997). Distributions of sensitivities to three sterol demethylation inhibitor fungicides among populations of Uncinula necator sensitive and resistant to triadimefon. Phytopathology 87, 784-791.
9. Evans, K. J., Scott, E. S. & Whisson, D. L. (1997a). Heterothallism among South Australian clonal lines of Uncinula necator. Australasian Plant Pathology 26, 10-20.
10. Evans, K. J., Whisson, D. L. & Scott, E. S. (1996). An experimental system for characterising isolates of Uncinula necator. Mycological Research 100, 675-680.
11. Evans, K. J., Whisson, D. L., Stummer, B. E. & Scott, E. S. (1997b). DNA markers identify variation in Australian populations of Uncinula necator. Mycological Research 101, 923-932.
12. Gadoury, D. M. & Pearson, R. C. (1991). Heterothallism and pathogenic specialization in Uncinula necator. Phytopathology 81, 1287-1293.
13. Goodwin, S. B., Saghai-Maroof, M. A. & Webster, R. K. (1993). Isozyme variation within and among populations of Rhynchosporium secalis in Europe, Australia and the United States. Mycological Research 97, 49-58.
14. Gubler, W. D., Ypema, H. L., Ouimette, D. G. & Bettiga, L. J. (1996). Occurrence of resistance in Uncinula necator to triadimefon, myclobutanil, and fenarimol in California grapevines. Plant Disease 80, 902-909.
15. Hantula, J., Lilja, A. & Parikka, P. (1997). Genetic variation and host specificity of Phytophthora cactorum isolated in Europe. Mycological Research 101, 565-572.
16. Lu, J., Knox, M. R., Ambrose, M. J., Brown, J. K. M. & Ellis, T. H. N. (1996). Comparative analysis of genetic diversity in pea assessed by RFLP-and PCR-based methods. Theoretical and Applied Genetics 93, 1103-1111.
17. McDonald, B. A. (1997). The population genetics of fungi: tools and techniques. Phytopathology 87, 448-453.
18. Miazzi, M., Natale, P., Pollastro, S. & Faretra, F. (1997). Handling of the biotrophic pathogen Uncinula necator (Schw.) Burr. under laboratory conditions and observations on its mating system. Journal of Plant Pathology 78, 71-77.
19. Nei, M. & Li, W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Science USA 76, 5269-5273.
20. O'Dell, M., Wolfe, M. S., Flavell, R. B., Simpson, C. G. & Summers, R. W. (1989). Molecular variation in populations of Erysiphe graminis on barley, oats and rye. Plant Pathology 38, 340-351.
21. Pearson, R. C. & Gadoury, D. M. (1987). Cleistothecia, the source of primary inoculum for grape powdery mildew in New York. Phytopathology 77, 1509-1514.
22. Pearson, R. C. & Gärtel, W. (1985). Occurrence of hyphae of Uncinula necator in buds of grapevine. Plant Disease 66, 149-151.
23. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, U.S.A.
24. Schlick, A., Kuhls, K., Meyer, W., Lieckfeldt, E., Börner, T. & Messner, K. (1994). Fingerprinting reveals gamma-ray induced mutations in fungal DNA: implications for identification of patent strains of Trichoderma harzianum. Current Genetics 26, 74-78.
25. Shannon, C. E. & Weaver, W. (1949). The Mathematical Theory of Communication. University of Illinois Press: Urbana, U.S.A.
26. Smith, C. G. (1970). Production of powdery mildew cleistocarps in a controlled environment. Transactions of the British Mycological Society 55, 355-365.
27. Stenlid, J., Karlsson, J.-O. & Högberg, N. (1994). Intraspecific genetic variation in Heterobasidion annosum revealed by amplification of minisatellite DNA. Mycological Research 98, 57-63.
28. Steva, H. (1994). Evaluating anti-resistance strategies for control of Uncinula necator. In Fungicide Resistance (ed. S. Heaney, D. Slawson, D. W. Hollomon, M. Smith, P. E. Russell & D. W. Parry), pp. 59-66. British Crop Protection Council Monograph No. 60.
29. Weining, S. & Langridge, P. (1991). Identification and mapping of polymorphisms in cereals based on the polymerase chain reaction. Theoretical and Applied Genetics 82, 209-216.
30. Weising, K., Atkinson, R. G. & Gardner, R. C. (1995). Genomic fingerprinting by microsatellite-primer PCR: a critical evaluation. PCR Methods and Applications 4, 249-255.
31. Wicks, T., Magarey, P. A. & Emmett, R. W. (1985). First report of Uncinula necator cleistothecia on grapevines in Australia. Plant Disease 69, 727.
32. Zietkiewicz, E., Rafalski, A. & Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20, 176-183.