Arabidopsis plants transformed with nitrilase 1 or 2 in antisense direction are delayed in clubroot development

Journal of Plant Physiology

Volumn 156, Issue 5-6, 2000, Pages 756-761

  Neuhaus, Klaus ^a   Grsic Rausch, Slobodanka ^a   Sauerteig, Sylvia ^a   Ludwig Müller, Jutta ^a,b  

^a GOETHE UNIVERSITY   (Germany)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Antisense transformation; Arabidopsis thaliana; Clubroot disease; Nitrilase; Plasmodiophora brassicae

Indexed keywords

ANTISENSE TECHNOLOGY; CLUBROOT DISEASE; FUNGAL INFECTION; FUNGAL SPORE; GENETIC TRANSFORMATION; INDOLEACETIC ACID; INFECTIVITY; MESSENGER RNA; NITRILASE; WILD RELATIVE;

ANIMALIA; ARABIDOPSIS; ARABIDOPSIS THALIANA; BACTERIA (MICROORGANISMS); FUNGI; PLASMODIOPHORA BRASSICAE;

EID: 0033918759     PISSN: 01761617     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0176-1617(00)80243-6     Document Type: Article

References (31)

1. Bartel, B., and G. R. Fink: Differential regulation of an auxin-producing nitrilase gene family in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 91, 6649-6653 (1994).
2. Bartling, D., M. Seedorf, A. Mithöfer and E. W. Weiler: Cloning and expression of an Arabidopsis nitrilase which can convert indole-3-acetonitrile to the plant hormone indole-3-acetic acid. Eur. J. Biochem. 205, 417-424 (1992).
3. Bartling, D., M. Seedorf, R. C. Schmidt and E. W. Weiler: Molecular characterization of two cloned nitrilases from Arabidopsis thaliana: Key enzymes in biosynthesis of the plant hormone indole-3-acetic acid. Proc. Natl. Acad. Sci. USA 91, 6021-6025 (1994).
4. Bischoff, M., R. Löw, S. Grsic, T. Rausch, W. Hilgenberg and J. Ludwig-Müller: Infection with the obligate biotroph Plasmodiophora brassicae, the causal agent of the clubroot disease, does not affect expression of NIT1/2-related nitrilases in roots of Chinese cabbage. J. Plant Physiol. 147, 341-345 (1995).
5. Butcher, D. N., S. El-Tigani and D. S. Ingram: The role of indole glucosinolates in the clubroot disease of the Cruciferae. Physiol. Plant. Pathol. 4, 127-141 (1974).
6. Butcher, D. N., K. Chamberlain, T. Rausch and L. M. Searle: Changes in indole metabolism during the development of clubroot symptoms in Brassicas. In: Biochemical aspects of synthetic and naturally occurring plant growth regulators, British Plant Growth Regulator Group, Monograph 11, 91-101 (1984).
7. Chen, K.-H., A. N. Miller, G. W. Patterson and J. D. Cohen: A rapid and simple procedure for the purification of indole-3-acetic acid prior to GC-SIM-MS analysis. Plant Physiol. 86, 822-825 (1988).
8. Dekhuijzen, H. M.: The occurence of free and bound cytokinins in plasmodia of Plasmodiophora brassicae isolated from tissue cultures of clubroots. Plant Cell Rep. 1, 18-20 (1981).
9. Dekhuijzen, H. M., and J. C. Overeem: The role of cytokinins in clubroot formation. Physiol. Plant. Pathol. 1, 151-161 (1971).
10. Grsic, S., B. Kirchheim, K. Pieper, M. Fritsch, W. Hilgenberg and J. Ludwig-Müller: Induction of auxin biosynthetic enzymes by jasmonic acid and in clubroot diseased Chinese cabbage plants. Physiol. Plant. 105, 521-531 (1999).
11. Grsic, S., S. Sauerteig, K. Neuhaus, M. Albrecht, J. Rossiter and J. Ludwig-Müller: Physiological analysis of transgenic Arabidopsis thaliana plants expressing one nitrilase isoform in sense or antisense direction. J. Plant Physiol. 153, 446-456 (1998).
12. Grsic-Rausch, S., P. Kobelt, J. Siemens, M. Bischoff and J. Ludwig-Müller: Expression and localization of nitrilase during symptom development of the clubroot disease in Arabidopsis thaliana. Plant Physiol. 122, (2000) in press.
13. Haughn, G. W., L. Davin, M. Giblin and E. W. Underbill: Biochemical genetics of plant secondary metabolites in Arabidopsis thaliana. The glucosinolates. Plant Physiol. 97, 217-226 (1991).
14. Ingram, D. S., and I. C. Tommerup: The life history of Plasmodiophora brassicae Woron. Proc. Royal. Soc. Lond. B 180, 103-112 (1972).
15. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685 (1970).
16. Logemann, J., J. Schell and L. Willmitzer: Improved method for the isolation of RNA from plant tissues. Anal. Biochem. 163, 16-20 (1987).
17. Löw, R., and T. Rausch: Sensitive non radioactive Northern Blots using alkaline transfer of total RNA and PCR-amplified biotinylated probes. BioTechniques 77, 1026-1030 (1994).
18. Ludwig-Müller, J.: Plasmodiophora brassicae, the causal agent of clubroot disease: a review on molecular and biochemical events in pathogenesis. Z. Pflanzenkr. Pflanzenschutz 106, 109-127 (1999).
19. Ludwig-Müller, J., U. Bendel, P. Thermann, M. Ruppel, E. Epstein and W. Hilgenberg: Concentrations of indole-3-acetic acid in plants of tolerant and susceptible varieties of Chinese cabbage infected with Plasmodiophora brassicae Woron. New Phytol. 125, 763-769 (1993).
20. Ludwig-Müller, J., E. Epstein and W. Hilgenberg: Auxin-conjugate hydrolysis in Chinese cabbage: Characterization of an amidohydrolase and its role during the clubroot disease. Physiol. Plant. 97, 627-634 (1996).
21. Ludwig-Müller, J., K. Pieper, M. Ruppel, J. D. Cohen, E. Epstein, G. Kiddle and R. Bennett: Indole glucosinolate and auxin biosynthesis in Arabidopsis thaliana L. glucosinolate mutants and the development of the clubroot disease. Planta 208, 409-419 (1999).
22. Müller, A., H. Hillebrand and E. W. Weiler: Indole-3-acetic acid is synthesized from L-tryptophan in roots of Arabidopsis thaliana. Planta 206, 362-369 (1998).
23. Müller, P., and W. Hilgenberg: Isomers of zeatin and zeatin riboside in clubroot tissue: evidence for trans-zeatin biosynthesis by Plasmodiophora brassicae. Physiol. Plant. 66, 245-250 (1986).
24. Normanly, J., and B. Bartel: Redundancy as a way of life - IAA metabolism. Curr. Opin. Plant Biol. 2, 207-213 (1999).
25. Normanly, J., J. D. Cohen and G. R. Fink: Arabidopsis thaliana auxotrophs reveal a tryptophan-independent biosynthetic pathway for indole-3-acetic acid. Proc. Natl. Acad. Sci. USA 90, 10355-10374 (1993).
26. Normanly, J., P. Grisafi, G. R. Fink and B. Bartel: Arabidopsis mutants resistant to the auxin effects of indole-3-acetonitrile are defective in the nitrilase encoded by the NIT1 gene. Plant Cell 9, 1781-1790 (1997).
27. Quirino, B., J. Normanly and R. M. Amasino: Diverse range of gene activity during Arabidopsis thaliana leaf senescence includes pathogen-independent induction of defense-related genes. Plant Mol. Biol. 40, 267-278 (1999).
28. Rausch, T., D. N. Butcher and W. Hilgenberg: Nitrilase activity in clubroot diseased plants. Physiol. Plant. 52, 467-470 (1981).
29. - Indole-3-methylglucosinolate biosynthesis and metabolism in clubroot diseased plants. Physiol. Plant. 58, 93-100 (1983).
30. Searle, L. M., K. Chamberlain, T. Rausch and D. N. Butcher: The conversion of 3-indolemethylglucosinolate to 3-indoleacetonitrile by myrosinase and its relevance to the clubroot disease of the cruciferae. J. Exp Bot. 33, 935-942 (1982).
31. +-ATPase of Mesembryanthemum crystallinum L. in the CAM state: a proteolytically processed subunit B? FEBS Lett. 389, 314-318 (1996).