Polyclonal-antibody-based ELISA to detect milk alkaline phosphatase

Journal of Agricultural and Food Chemistry

Volumn 48, Issue 6, 2000, Pages 2087-2091

  Vega Warner, A Virginia ^a   Gandhi, Hasand ^a   Smith, Denise M ^a   Ustunol, Zeynep ^a  

^a MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

Alkaline phosphatase; Antibodies; ELISA; Milk

Indexed keywords

ALKALINE PHOSPHATASE; ANTIBODY; MILK; MILK PROTEIN; POLYCLONAL ANTIBODY;

ANIMAL; ANTIBODY SPECIFICITY; ARTICLE; CATTLE; CROSS REACTION; ENZYME ASSAY; ENZYME DENATURATION; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYMOLOGY; EPIDEMIOLOGY; FEMALE; METHODOLOGY; NONHUMAN; PASTEURIZATION; PLACENTA; PREGNANCY;

ALKALINE PHOSPHATASE; ANIMALS; ANTIBODIES; CATTLE; CROSS REACTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; MILK; PLACENTA; PREGNANCY; SENSITIVITY AND SPECIFICITY;

EID: 0033916567     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf990412v     Document Type: Article

References (18)

1. Bergmeyer, H. U. In Methods of Enzymatic Analysis, 3rd ed.; Verlag Chemie Int.: Deerfield Beach, FL, 1991; Vol. 4.
2. Deshpande, S. S. Assay development, evaluation, and validation. In Enzyme Immunoassays: From Concept to Product Development; Chapman and Hall: New York, 1996.
3. Harlow, E.; Lane, D. In Antibodies, a laboratory manual; Cold Spring Harbor Laboratory: Cold Spring Harbor, NY, 1988.
4. International Dairy Federation. Alkaline phosphatase test as a measure of correct pasteurization. Bull. IDF 1991, 262, 33-35.
5. Kay, H. D.; Graham, W. R. J. The phosphatase test for pasteurized milk. J. Dairy Res. 1935, 6, 191-203.
6. Kleyn, D. H.; Lin, S. H. C. Collaborative study of a new alkaline phosphatase assay system for milk. J. Assoc. Off. Anal. Chem. 1968, 51, 802-807.
7. Knight, A. H.; Fryer, S. M. The development of heat-resistant phosphatase activity in raw milk. J. Soc. Dairy Technol. 1989, 42 (3), 81-86.
8. McComb, R. B.; Bowers, G. N.; Posen, S. Structural features. In Alkaline Phosphatase; Plenum Press: New York, 1979.
9. Murthy, G. K.; Kaylor, L. O. Evaluation of APHA and AOAC II methods for phosphatase in butter and differentiation of milk and microbial phosphatases by agarose-gel electrophoresis. J. Assoc. Offic. Anal. Chem. 1990, 73, 681-687.
10. Murthy, G. K.; Kleyn, D. H.; Richardson, T.; Rocco, R. M. Alkaline phosphatase methods. In Standards Methods for the Examination of Dairy Products; Marshall, R., Ed.; American Public Health Association: Washington, DC, 1992.
11. Painter, C. J.; Bradley, R. L., Jr. Residual alkaline phosphatase activity in milks subjected to various time-temperature treatments. J. Food Prot. 1997, 60, 525-530.
12. Pasteurized Milk Ordinance. U. S. Department of Health and Human Services, Public Health Service/Food and Drug Administration, Washington, DC, 1993. Publ. No. 229.
13. Peereboom, J. W. C. Studies on alkaline milk phosphatase II occurrence of various phosphatase isozymes in dairy products. Neth. Milk Dairy J. 1968, 22, 137-152.
14. Rocco, R. M. Fluorometric analysis of alkaline phosphatase in fluid dairy products. J. Food Prot. 1990, 53, 588-591.
15. Sharma, R. S.; Ganguli, N. C. Alkaline phosphatase of milk: A sialoprotein. Indian J. Dairy Sci. 1969, 22, 272-274.
16. Vega-Warner, A. V.; Wang, C.-H.; Smith, D. M.; Ustunol, Z. Bovine milk alkaline phosphatase: Purification and production of polyclonal antibodies. J. Food Sci. 1999, 64, 601-605.
17. Wang, C.-H.; Abouzied, M. M.; Pestka, J. J.; Smith, D. M. Antibody development and enzyme-linked immunosorbent assay for the protein marker lactate dehydrogenase to determine safe cooking end-point temperatures of turkey rolls. J. Agric. Food Chem. 1992, 40, 1671-1676.
18. Whitney, R. M. Proteins of milk. In Fundamentals of Dairy Chemistry; Wong, N. P., Jenness, R., Keeney, M., Marth, E. H., Eds.; Van Nostrand Reinhold Co.: New York, 1988.