Bypassing hybridoma technology: HLA-C reactive human single-chain antibody fragments (scFv) derived from a synthetic phage display library (HuCAL) and their potential to discriminate HLA class I specificities

Tissue Antigens

Volumn 56, Issue 1, 2000, Pages 1-9

  Marget, Matthias ^a   Sharma, B B ^b   Tesar, M ^c   Kretzschmar, T ^c   Jenisch, S ^a   Westphal, E ^a   Davarnia, P ^a   Weiss, E ^d   Ulbrecht, M ^d   Kabelitz, D ^a   Kronke M ^e  

^a UNIVERSITY OF KIEL   (Germany)

^b LA JOLLA INSTITUTE FOR ALLERGY AND IMMUNOLOGY   (United States)

^c MORPHOSYS AG   (Germany)

^d Inst fur Anthropologie/H   (Germany)

^e Inst fur Med Mikrobiol I   (Germany)

Author keywords

Cw*0602; Histocompatibility testing; HLA typing; HLA C; Monoclonal antibody; Phage display; ScFv; Single chain antibody; Whole cell panning

Indexed keywords

ANTIBODY; HLA ANTIGEN CLASS 1; HLA C ANTIGEN; HUMAN MONOCLONAL ANTIBODY;

ALLELE; ANTIGEN SPECIFICITY; ARTICLE; CELL LYSATE; CONTROLLED STUDY; DNA TRANSFECTION; FLOW CYTOMETRY; GENE OVEREXPRESSION; GENETIC ENGINEERING; HISTOCOMPATIBILITY TEST; HLA TYPING; HUMAN; HUMAN CELL; HYBRIDOMA; IMMUNOBLOTTING; IMMUNOPRECIPITATION; LYMPHOCYTE; PHAGE DISPLAY; PRIORITY JOURNAL;

ALLELES; ANTIBODY SPECIFICITY; CELL LINE; FLOW CYTOMETRY; HISTOCOMPATIBILITY ANTIGENS CLASS I; HLA-C ANTIGENS; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; LYMPHOCYTES; PEPTIDE LIBRARY; PRECIPITIN TESTS; TRANSFECTION;

EID: 0033912255     PISSN: 00012815     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-0039.2000.560101.x     Document Type: Article

References (33)

1. 1. Dyer PA, Middelton D, eds. Histocompatibility testing: a practical approach. Oxford: IRL Press, 1993.
2. 2. Mulder A, Kardol MJ, Uit het Broek CM, Tanke-Visser J, Young NT, Claas FH. A human monoclonal antibody against HLA-Cw1 and a human monoclonal antibody against an HLA-A locus determinant derived from a single uniparous female. Tissue Antigens 1998: 52: 393-6.
3. 3. Grassi F, Meneveri R, Gullberg M et al. Human immunodeficiency virus type 1 gp120 mimics a hidden monomorphic epitope borne by class I major histocompatibility complex heavy chains. J Exp Med 1991: 174: 53-62.
4. 4. Giacomini P, Beretta A, Nicotra MR et al. HLA-C heavy chains free of beta2-microglobulin: distribution in normal tissues and neoplastic lesions of non-lymphoid origin and interferon-gamma responsiveness. Tissue Antigens 1997: 50: 555-66.
5. 5. Smith GP. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 1985: 228: 1315-7.
6. 6. Skerra A, Pluckthun A. Assembly of a functional immunoglobulin Fv fragment in Escherichia coli. Science 1988: 240: 1038-41.
7. 7. McCafferty J, Griffiths AD, Winter G, Chiswell DJ. Phage antibodies: filamentous phage displaying antibody variable domains. Nature 1990: 348: 552-4.
8. 8. Kettleborough CA, Ansell KH, Allen RW, Rosell-Vives E, Güssow DH, Bendig MM. Isolation of tumor cell-specific single chain Fv from immunized mice using phage-antibody libraries and the re-construction of whole antibodies from these antibody fragments. Eur J Immunol 1994: 24: 952-8.
9. 9. Marks JD, Hoogenboom HR, Bonnert TP, McCafferty J, Griffiths AD, Winter G. Bypassing immunization. Human antibodies from V-gene libraries displayed on phage. J Mol Biol 1991: 222: 581-97.
10. 10. Griffiths AD, Duncan AR. Strategies for selection of antibodies by phage display. Curr Opin Biotechnol 1998: 9: 102-8.
11. 11. Winter G, Griffiths AD, Hawkins RE, Hoogenboom HR. Making antibodies by phage display technology. Annu Rev Immunol 1994: 12: 433-55.
12. 12. Clackson T, Hoogenboom HR, Griffiths AD, Winter G. Making antibody fragments using phage display libraries. Nature 1991: 352: 624-8.
13. 13. Williamson RA, Burioni R, Sanna PP, Partridge LJ, Barbas CF III, Burton DR. Human monoclonal antibodies against a plethora of viral pathogens from single combinatorial libraries. Proc Natl Acad Sci U S A 1993: 90: 4141-5.
14. 14. Griffiths AD, Malmqvist M, Marks JD et al. Human anti-self antibodies with high specificity from phage display libraries. EMBO J 1993: 12: 725-34.
15. 15. Kanof ME, Smith PD. Preparation of human mononuclear cell populations and subpopulations. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, Coico R, eds. Current protocols in immunology. New York: John Wiley & Sons, 1994: 7.1.1-7.1.5.
16. 16. Shimizu Y, DeMars R. Production of human cells expressing individual transferred HLA-A,-B,-C genes using an HLA-A,-B,-C null human cell line. J Immunol 1989: 142: 3320-8.
17. 17. Sambrook J, Fritsch EF, Maniatis T, eds. Molecular cloning: A laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989.
18. 18. Weiss EH, Bloemer K, Doerner C et al. Molecular biology of the HLA-B27 locus. Br J Rheumatol 1988: 27 (Suppl 2): 12-8.
19. 19. Stam NJ, Vroom TM, Peters PJ, Pastoors EB, Ploegh HL. HLA-A-and HLA-B-specific monoclonal antibodies reactive with free heavy chains in western blots, in formalin-fixed, paraffin-embedded tissue sections and in cryo-immuno-electron microscopy. Int Immunol 1990: 2: 113-25.
20. 20. Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 1985: 5: 3610-6.
21. 21. Brodsky FM, Parham P, Barnstable CJ, Crumpton MJ, Bodmer WF. Monoclonal antibodies for analysis of the HLA system. Immunol Rev 1979: 47: 3-61.
22. 22. Holmes N, Fowlkes DM. Preparation of cells and reagents for flow cytometry. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, Coico R, eds. Current protocols in immunology. New York: John Wiley & Sons, 1994: 5.3.1-5.3.23.
23. 23. Knappik A, Ge L, Honegger A et al. Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. J Mol Biol 2000: 296: 57-86.
24. 24. Inoue H, Nojima H, Okayama H. High efficiency transformation of Escherichia coli with plasmids. Gene 1991: 96: 23-8.
25. 25. Zemmour J. Inefficient assembly limits transport and cell surface expression of HLA-Cw4 molecules in C1R. Tissue Antigens 1996: 48: 651-61.
26. 26. McCutcheon JA, Gumperz J, Smith KD, Lutz CT, Parham P. Low HLA-C expression at cell surfaces correlates with increased turnover of heavy chain mRNA. J Exp Med 1995: 181: 2085-95.
27. 27. Pistillo MP, Hammer J, Bono E et al. A novel approach to human anti-HLA mABs production: use of phage display libraries. Hum Immunol 1997: 57: 19-26.
28. 28. Pistillo MP, Hammer J, Bono E et al. Isolation of scFv fragments to polymorphic and monomorphic HLA-DR epitopes from a synthetic phage library. Exp Clin Immunogenet 1997: 14: 123-30.
29. 29. Colonna M, Borsellino G, Falco M, Ferrara G-B, Strominger JL. HLA-C is the inhibitory ligand that determines dominant resistance to lysis by NK1-and NK2-specific natural killer cells. Proc Natl Acad Sci U S A 1993: 90: 12000-4.
30. 30. Mandelboim O, Reyburn HT, Sheu EG et al. The binding site of NK receptors on HLA-C molecules. Immunity 1997: 6: 341-50.
31. 31. Falk K, Rotzschke O, Grahovac B et al. Allele-specific peptide ligand motifs of HLA-C molecules. Proc Natl Acad Sci U S A 1993: 90: 12005-9.
32. 32. King A, Burrows T, Loke YW. Human uterine natural killer cells. Nat Immun 1996: 15: 41-52.
33. 33. King A, Boocock C, Sharkey AM et al. Evidence for the expression of HLA-C class I mRNA and protein by human first trimester trophoblast. J Immunol 1996: 156: 2068-76.