Transgenic tobacco plants co-expressing Agrobacterium iaa and ipt genes have wild-type hormone levels but display both auxin- and cytokinin-overproducing phenotypes

Plant Journal

Volumn 23, Issue 2, 2000, Pages 279-284

  Eklöf, Staffan ^a   Åstot, Crister ^a   Sitbon, Folke ^a   Moritz, Thomas ^a   Olsson, Olof ^b   Sandberg, Göran ^a  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Cytokinin; IAA; Morphology; Nicotiana tabacum; Ratio hypothesis; Transgenic plants

Indexed keywords

AUXIN; CROSSING; CYTOKININ; GENE EXPRESSION; GENETIC CROSS; GENETIC TRAIT; IAAH GENE; IAAM GENE; INDOLEACETIC ACID; IPT GENE; TOBACCO; TRANSGENIC PLANT; WILD RELATIVE; ZEATIN RIBOSIDE 5 MONOPHOSPHATE; ZEATIN RIBOSIDE;

ADENOSINE; ALKYL AND ARYL TRANSFERASES; BACTERIAL PROTEINS; CROSSES, GENETIC; CYTOKININS; INDOLEACETIC ACIDS; ISOPENTENYLADENOSINE; PHENOTYPE; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; RHIZOBIUM; TOBACCO;

AGROBACTERIUM; BACTERIA (MICROORGANISMS); EMBRYOPHYTA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; TABACUM;

EID: 0033907065     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2000.00762.x     Document Type: Article

References (22)

1. Doyle, J.J. and Doyle, J.L. (1990) Isolation of plant DNA from fresh tissue. Focus, 12, 13-15.
2. Edlund, A., Eklöf, S., Sundberg, B., Moritz, T. and Sandberg, G. (1995) A microscale technique for gas chromatography-mass spectrometry measurements of picogram amounts of indole-3-acetic acid in plant tissues. Plant Physiol. 108, 1043-1047.
3. Eklöf, S., Åstot, C., Blackwell, J., Moritz, T., Olsson, O. and Sandberg, G. (1996) Cytokinin metabolites and gradients in wild type and transgenic tobacco with moderate cytokinin overproduction. Physiol. Plant. 98, 333-344.
4. Eklöf, S., Åstot, C., Blackwell, J., Moritz, T., Olsson, O. and Sandberg, G. (1997) Auxin-cytokinin interactions in wild-type and transgenic tobacco. Plant Cell Physiol. 33, 225-235.
5. Klee, H. and Estelle, M. (1991) Molecular genetic approaches to plant hormone biology. Ann. Rev. Plant Physiol. Mol. Biol. 42, 529-551.
6. Klee, H.J., Horsch, R.B., Hinchee, M.A., Hein, M.B. and Hoffman, N.L. (1987) The effects of overproduction of two Agrobacterium tumefaciens T-DNA auxin biosynthetic gene products in transgenic petunia plants. Genes Dev. 1, 86-96.
7. Medford, J.I., Morgan, R., El-Sawi, Z. and Klee, H.J. (1989) Alterations of endogenous cytokinins in transgenic plants using a chimeric isopentenyl transferase gene. Plant Cell, 1, 403-413.
8. Neumann, C. and Cohen, S. (1997) Morphogens and pattern formation. Bioassay, 19, 721-729.
9. Nordström, A.-C. and Eliasson, L. (1991) Levels of endogenous indole-3-acetic acid and indole-3-acetylaspartic acid during adventitious root formation in pea cuttings. Physiol. Plant. 82, 599-605.
10. Odell, J.T., Nagy, F. and Chua, N.H. (1985) Identification of DNA sequences required for activity of the cauliflower mosaic 35S promoter. Nature, 313, 810-812.
11. Ondrej, M., Bavrina, T.V., Dudko, N., Hrouda, M., Krekule, J., Lozhnivkova, V.N., Machakova, I., Seidlova, F. and Vlasak, J. (1991) Transgenic tobacco plants with T-DNA phytohormone synthesis genes. Biol. Plant. 33, 40-48.
12. Ow, D.W., Jacobs, J.D. and Howell, S.H. (1987) Functional regions of the cauliflower mosaic virus 35S promoter determined by use of the firefly luciferase gene as a reporter of promoter activity. Proc. Natl Acad. Sci. USA, 84, 4870-4874.
13. Romano, C.P., Hein, M.B. and Klee, H.J. (1991) Inactivation of auxin in tobacco transformed with the indoleacetic acid-lysine synthetase gene of Pseudomonas savastanoi. Genes Devel. 5, 438-446.
14. Sitbon, F., Åstot, C., Edlund, A., Crozier, A. and Sandberg, G. (2000) The relative importance of tryptophan-dependent and tryptophan-independent IAA biosynthesis in tobacco during vegetative growth. Planta, in press.
15. Sitbon, F., Hennion, S., Sundberg, B., Little, C.H.A., Olsson, O. and Sandberg, G. (1992) Transgenic tobacco plants coexpressing the Agrobacterium tumefaciens iaaM and iaaH genes display altered growth and indole-3-acetic acid metabolism. Plant Physiol. 99, 1062-1069.
16. Sitbon, F., Östin, A., Sundberg, B., Olsson, O. and Sandberg, G. (1993) Conjugation of indole-3-acetic acid (IAA) in wild-type and IAA-overproducing transgenic tobacco plants, and identification of the main conjugates by frit-fast atom bombardment liquid chromatography-mass spectrometry. Plant Physiol. 101, 313-320.
17. Sitbon, F., Sundberg, B., Olsson, O. and Sandberg, G. (1991) Free and conjugated indoleacetic acid (IAA) contents in transgenic tobacco plants expressing the iaaM and iaaH IAA biosynthesis genes from Agrobacterium tumefaciens. Plant Physiol. 95, 480-485.
18. Skoog, F. and Miller, C.O. (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. Exp. Biol. 11, 118-131.
19. Smigocki, A.C. and Owens, L.D. (1989) Cytokinin-to-auxin ratios and morphology of shoots and tissues transformed by a chimeric isopentenyl transferase gene. Plant Physiol. 91, 808-811.
20. Wickson, M. and Thimann, K.V. (1958) The antagonism of auxin and kinetin in apical dominance. Physiol. Plant. 11, 62-74.
21. Wright, A.D., Sampson, M.B., Neuffer, G., Michalczuk, L., Slovin, J.P. and Cohen, J.D. (1991) Indole-3-acetic acid biosynthesis in the mutant maize orange pericarp, a tryptophan auxotroph. Science, 254, 998-1000.
22. Yusibov, V.M., II, P.C., Andrianov, V.M. and Piruzian, E.S. (1991) Phenotypically normal transgenic T-cyt tobacco plants as a model for the investigation of plant gene expression in response to phytohormonal stress. Plant Mol. Biol. 17, 825-836.