Episomal expression of a hammerhead ribozyme directed against plum pox virus

Virus Research

Volumn 68, Issue 1, 2000, Pages 15-23

  Liu, Bolin ^a,b   Tabler, Martin ^a   Tsagris, Mina ^a,c  

^a Inst of Molec Biol and Biotech   (Greece)

^b INSTITUTE OF BOTANY   (China)

^c UNIVERSITY OF CRETE   (Greece)

Author keywords

Antisense RNA; Catalytic RNA; Double stranded RNA; Potato virus X vector; Potyvirus

Indexed keywords

COMPLEMENTARY RNA; PLANT RNA; RIBOZYME;

AGRICULTURE; ARTICLE; CELL PROTECTION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; EPISOME; INOCULATION; MOSAIC VIRUS; NONHUMAN; PLANT; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; VIRUS RECOMBINANT;

GENETIC VECTORS; PLANTS, TOXIC; PLASMIDS; PLUM POX VIRUS; POTEXVIRUS; RECOMBINATION, GENETIC; RNA, ANTISENSE; RNA, CATALYTIC; RNA, DOUBLE-STRANDED; TOBACCO; VIRUS REPLICATION;

EID: 0033898799     PISSN: 01681702     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1702(00)00145-3     Document Type: Article

References (40)

1. Anandalakshmi R., Pruss G.J., Ge X., Marathe R., Mallory A.C., Smith T.H., Vance V.B. A viral suppressor of gene silencing in plants. Proc. Natl. Acad. Sci. USA. 95:1998;13079-13084.
2. Bejarano E.R., Lichtenstein C.P. Expression of TGMV antisense RNA in transgenic tobacco inhibits replication of BCTV but not ACMV geminiviruses. Plant Mol. Biol. 24:1994;241-248.
3. Brigneti G., Voinnet O., Li W.X., Ji L.H., Ding S.W., Baulcombe D.C. Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J. 17:1998;6739-6746.
4. Birikh K.R., Heaton P.A., Eckstein F. The structure, function and application of the hammerhead ribozyme. Eur. J. Biochem. 245:1997;1-16.
5. Chapman S., Kavanagh T., Baulcombe D. Potato virus X as a vector for gene expression in plants. Plant J. 2:1992;549-557.
6. Day A.G., Bejarano E.R., Buck K.W., Burrell M., Lichtenstein C.P. Expression of an antisense viral gene in transgenic tobacco confers resistance to the DNA virus tomato golden mosaic virus. Proc. Natl. Acad. Sci. USA. 88:1991;6721-6725.
7. de Feyter R., Young M., Schroeder K., Dennis E.S., Gerlach W. A ribozyme gene and antisense gene are equally effective in conferring resistance to tobacco mosaic virus. Mol.Gen. Genet. 250:1996;329-338.
8. Fire A., Xu S.Q., Montgomery M.K., Kostas S.A., Driver S.E., Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 391:1998;806-811.
9. Goodman R.M., Ross A.F. Enhancement of potato virus X synthesis in doubly infected tobacco occurs in doubly infected cells. Virology. 58:1974;16-24.
10. Heinrich J.-C., Tabler M., Louis C. Attenuation of white gene expression in transgenic Drosophila melanogaster: possible role of a catalytic antisense RNA. Dev. Genet. 14:1993;258-265.
11. Heinrich J.-C., Tabler M., Louis C. The influence of chromosomal position and copy number of a white-directed ribozyme gene on the suppression of eye pigmentation in Drosophila melanogaster. Antisense Res. Dev. 5:1995;155-160.
12. Homann M., Tzortzakaki S., Rittner K., Sczakiel G., Tabler M. Incorporation of the catalytic domain of a hammerhead ribozyme into antisense RNA enhances its inhibitory effect on the replication of human immunodeficiency virus type 1. Nucl. Acids Res. 21:1993;2809-2814.
13. Hormes R., Homann M., Oelze I., Marschall P., Tabler M., Eckstein F., Sczakiel G. The subcellular localization and length of hammerhead ribozymes determine efficacy in human cells. Nucleic Acids Res. 15:1997;769-775.
14. Kasschau K.D., Carrington J.C. A counterdefensive strategy of plant viruses: suppression of posttranscriptional gene silencing. Cell. 95:1998;461-470.
15. Kwon C.S., Chung W.I., Paek K.H. Ribozyme mediated targeting of cucumber mosaic virus RNA 1 and 2 in transgenic tobacco plants. Mol. Cells. 7:1997;326-334.
16. Larsson S., Hotchkiss G., Andäng M., Nyholm T., Inzunza J., Jansson I., Ährlund-Richter L. Reduced β-microglobulin mRNA level in transgenic mice expressing a designed hammerhead ribozyme. Nucleic Acids Res. 22:1994;2242-2248.
17. Martin M.T., Ceroera M.T., Garcia J.A. Properties of the active PPV RNA polymerase complex in defined glycerol gradient fractions. Virus Res. 37:1995;127-137.
18. Matthews R.E. Plant Virology. third edition :1991;Academic Press, San Diego, USA.
19. Mazzolini L., Axelos M., Lescure N., Yot P. Assaying synthetic ribozymes in plants: high level expression of a functional hammerhead structure fails to inhibit target gene activity in transiently transformed protoplasts. Plant Mol. Biol. 20:1992;715-731.
20. McIntyre C.L., Bettenay H.M., Manners J.M. Strategies for the suppression of peroxidase gene expression in tobacco. II. In vivo suppression of peroxidase activity in transgenic tobacco using ribozyme and antisense constructs. Transgenic Res. 5:1996;263-270.
21. Merlo A.O., Cowen N., Delate T., Edington B., Folkerts O., Hopkins N., Lemeiux C., Skokut T., Smith K., Woosley A., Yang Y., Young S., Zwick M. Ribozymes targeted to stearoyl-ACP Δ9 desaturase mRNA produce heritable increases of stearic acid in transgenic maize leaves. Plant Cell. 10:1998;1603-1622.
22. Perriman R., Graf L., Gerlach W.L. A ribozyme that enhances gene suppression in tobacco protoplasts. Antisense Res. Dev. 3:1993;253-263.
23. Perriman R., Bruening G., Dennis E.S., Peacock W.J. Effective ribozyme delivery in plant cells. Proc. Natl. Acad. Sci. USA. 92:1995;6175-6179.
24. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning A Laboratory Manual. 1989;Cold Spring Habor Laboratory Press, New York.
25. Steinecke P., Herget T., Schreier P.H. Expression of a chimeric ribozyme gene results in endonucleolytic cleavage of target mRNA and a concomitant reduction of gene expression in vivo. EMBO J. 11:1992;1525-1530.
26. Sullenger B.A., Chech T. Tethering ribozymes to a retroviral packaging signal for destruction of viral RNA. Science. 262:1993;1566-1569.
27. Symons R.H. Small catalytic RNAs. Annu. Rev. Biochem. 61:1992;641-671.
28. Symons R.H. Plant pathogenic RNAs and RNA catalysis. Nucleic Acids Res. 15:1997;2683-2689.
29. Tabler M. Antisense RNA in plants: a tool for analysis and suppression of gene function. Roubelakis-Angelakis K.A., Tran thanh Van K. Morphogenesis in Plants: Molecular Approaches, NATO ASI Series. 1993;137-258 Plenum Press, London.
30. Tabler M., Tsagris M. Catalytic antisense RNAs produced by incorporating ribozyme cassettes into cDNA. Gene. 108:1991;175-183.
31. Tabler M., Günther I., Kern R., Sänger H.L. A microscale procedure for isolating and sequencing the viroid RNA present in one gram of infected leaf tissue. J. Virol. Methods. 23:1989;111-126.
32. Tabler M., Homann M., Tzortzakaki S., Sczakiel G. A three-nucleotide helix I is sufficient for full activity of a hammerhead ribozyme: advantages of an saymmetric design. Nucleic Acids Res. 22:1994;3958-3965.
33. Techeney P.Y., Tavert G., Delbos R.P., Ravelonandro M., Dunez J. The complete nucleotide sequence of plum pox virus RNA (strain D). Nucleic Acids Res. 17:1989;10115-10116.
34. Tsagris M., Tabler M., Mühlbach H.P., Sänger H.L. Linear oligometric potato spindle tuber viroid (PSTV) RNAs are accurately processed in vitro to the monometric circular viroid proper when incubated with a nuclear extract from healthy potato cells. EMBO J. 8:1987;2173-2183.
35. Vaish N.K., Kore A.R., Eckstein F. Recent developments in the hammerhead ribozyme field. Nucleic Acids Res. 26:1998;5237-5242.
36. Vance V.B., Gerger P.H., Carrington J.C., Hunt A.G., Shi X.M. 5′ Proximal potyviral sequence mediate potato virus X/potyviral synergistic disease in transgenic tobacco. Virology. 206:1995;583-590.
37. Waterhouse P.W., Graham M.W., Wang M.B. Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc. Natl. Acad. Sci. USA. 95:1998;13959-13964.
38. Wegener D., Steinecke P., Herget T., Petereit I., Philipp C., Schreier P. Expression of a reporter gene is reduced by a ribozyme in transgenic plants. Mol. Gen. Genet. 245:1994;465-470.
39. Yang X., Yie Y., Zhu F., Liu F., Kang L., Wang X., Po T. Ribozyme-mediated high resistance against potato spindle tuber viroid in transgenic potatoes. Proc. Natl. Acad. Sci. USA. 94:1997;4861-4865.
40. Zhao J.J., Pick L. Generation loss-of function phenotypes of the fushi tarazu gene with a targeted ribozyme in Drosophila. Nature. 365:1993;448-451.