Ribozymes targeted to stearoyi-ACP Δ9 desaturase mRNA produce heritable increases of stearic acid in transgenic maize leaves

Plant Cell

Volumn 10, Issue 10, 1998, Pages 1603-1621

  Merlo, Ann Owens ^a   Cowen, Neil ^a   Delate, Tom ^b   Edington, Brent ^b,d   Folkerts, Otto ^c   Hopkins, Nicole ^a   Lemeiux, Christine ^b   Skokut, Tom ^a   Smith, Kelley ^a   Woosley, Aaron ^a   Yang, Yajing ^a   Young, Scott ^b   Zwick, Michael ^b  

^a DOW AGROSCIENCES LLC   (United States)

^b Sirna Therapeutics Inc   (United States)

^c CURAGEN CORPORATION   (United States)

^d Ardmore Biological Analysis   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EMBRYOPHYTA; ZEA MAYS;

ACYL (ACYL CARRIER PROTEIN)DESATURASE; ACYL-(ACYL-CARRIER-PROTEIN)DESATURASE; COMPLEMENTARY DNA; FATTY ACID; MESSENGER RNA; MIXED FUNCTION OXIDASE; PLANT DNA; PLANT RNA; RIBOZYME; STEARIC ACID; STEARIC ACID DERIVATIVE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; GENETICS; ISOLATION AND PURIFICATION; MAIZE; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHENOTYPE; PLANT LEAF; PLANT SEED; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; DNA, COMPLEMENTARY; DNA, PLANT; FATTY ACIDS; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; PHENOTYPE; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; RNA, CATALYTIC; RNA, MESSENGER; RNA, PLANT; SEEDS; STEARIC ACIDS; ZEA MAYS;

EID: 0032185811     PISSN: 10404651     EISSN: None     Source Type: Journal    
DOI: 10.1105/tpc.10.10.1603     Document Type: Article

References (65)

1. Akagi, H., Baba, T., Shimada, H., and Fufimura, T. (1995). Nucleotide sequence of a stearoyl-acyl carrier protein desaturase cDNA from developing seeds of rice. Plant Physiol. 108, 845-846.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K., eds (1989). Current Protocols in Molecular Biology. (New York: Greene Publishing Associates and Wiley Interscience).
3. Bennett, J.M., and Cullimore, J.V. (1992). Selective cleavage of closely-related mRNAs by synthetic ribozymes. Nucleic Acids Res. 20, 831-837.
4. Bertrand, E., Grange, T., and Pictet, R. (1992). trans-Acting hammerhead ribozymes in vivo present limits and future direction. In Gene Regulation: Biology of Antisense RNA and DNA, R.P. Erickson and J.G. Izant, eds (New York: Raven Press), pp. 71-81.
5. Borovkov, A.Y., McClean, P.E., Sowokinos, J., Rund, S.H., and Secor, G.A. (1996). Effect of expression of UDP-glucose pyrophosphorylase ribozyme and antisense RNAs on the enzyme activity and carbohydrate composition of field-grown transgenic potato plants. J. Plant Physiol. 147, 644-652.
6. Bourque, J.E. (1995). Antisense strategies for genetic manipulations in plants. Plant Sci. 105, 125-149.
7. Browse, J., McCourt, P.J., and Somerville, C.R. (1986). Fatty acid composition of leaf lipids determined after combined digestion and fatty acid methyl ester formation from fresh tissue. Anal. Biochem. 152, 141-145.
8. Cameron, F.H., and Jennings, P.A. (1989). Specific gene suppression by engineered ribozymes in monkey cells. Proc. Natl. Acad. Sci. USA 86, 9139-9143.
9. Christoffersen, R.E., McSwiggen, J.A., and Konings, D. (1994). Applications of computational technology to ribozyme biotechnology products. J. Theor. Chem. 311, 273-284.
10. Chu, C.C., Wang, C.C., Sun, C.S., Hsu, C., Yin, K.C., Chu, C.Y., and Bi, F.Y. (1975). Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sin. 18, 659-668.
11. De Boer, A.D., and Weisbeek, P.J. (1991). Chloroplast topogenesis: Import, sorting and assembly. Biochim. Biophys. Acta 1071, 221-253.
12. de Feyter, R., Young, M., Schroeder, K., Dennis, E.S., and Gerlach, W. (1996). A ribozyme gene and an antisense gene are equally effective in conferring resistance to tobacco mosaic virus on transgenic tobacco. Mol. Gen. Genet. 250, 329-338.
13. DeLeon, D.V., Cox, K.M., Angerer, L.M., and Angerer, R.C. (1983). Most early-variant histone mRNA is contained in the pronucleus of sea urchin eggs. Dev. Biol. 100, 197-206.
14. Fawcett, T., Simon, W.J., Swinhoe, R., Shanklin, J., Nishida, I., Christie, W.W., and Slabas, A.R. (1994). Expression of mRNA and steady-state levels of protein isoforms of enoyl-ACP reductase from Brassica napis. Plant Mol. Biol. 26, 155-163.
15. Finnegan, J., and McElroy, D. (1994). Transgene inactivation: Plants fight back. Bio/Technology 12, 883-888.
16. Forster, A.C., and Symons, R.H. (1987). Self-cleavage of plus and minus RNAs of a virusoid and a structural model for the active sites. Cell 49, 211-220.
17. Graef, G.L., Fehr, W.R., and Hammond, E.G. (1985). Inheritance of three stearic acid mutants of soybean. Crop Sci. 25, 1076-1079.
18. Hammond, E.G., and Fehr, W.R. (1984). Improving the fatty acid composition of soybean oil. J. Am. Oil Chem. Soc. 61, 1713-1716.
19. Haseloff, J., and Gerlach, W.L. (1988). Simple RNA enzymes with new and highly specific endoribonuclease activity. Nature 334, 585-591.
20. Herschlag, D., and Cech, T.R. (1990). Catalysis of RNA cleavage by the Tetrahymena thermophila ribozyme. Biochemistry 29, 10159-10171.
21. Hertel, K.J., Pardi, A., Uhlenbeck, O.C., Koizumi, M., Ohtsuka, E., Uesugi, S., Cedegren, R., Eckstein, F., Gerlach, W.L., Hodgson, R., and Symons, R.H. (1992). Numbering system for the hammerhead. Nucleic Acids Res. 20, 3252.
22. Jarvis, T.C., Alby, L.J., Beaudry, A.A., Wincott, F.E., Beigelman, L., McSwiggen, J.A., Usman, N., and Stinchcomb, D.T. (1996). Inhibition of vascular smooth muscle cell proliferation by ribozymes that cleave c-myb mRNA. RNA 2, 419-428.
23. Knutzon, D.S., Thompson, G.A., Radke, S.E., Johnson, W.B., Knauf, V.C., and Kridl, J.C. (1992). Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Proc. Natl. Acad. Sci. USA 89, 2624-2628.
24. Koizumi, M., Iwai, S., and Ohtsuka, E. (1938). Construction of a series of several self-cleaving RNA duplexes using synthetic 21mers. FEBS Lett. 2, 228-230.
25. Kruger, K., Grabowski, P.J., Zang, A.J., Sands, J., Gottschling, D.E., and Cech, T.R. (1982). Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena. Cell 31, 147-157.
26. Ladd, S.L., and Knowles, P.F. (1970). Inheritance of stearic acid in the seed oil of safflower. Crop Sci. 10, 525-527.
27. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
28. Lindqvist, Y., Huang, W., Schneider, G., and Shanklin, J. (1996). Crystal structure of Δ9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other di-iron proteins. EMBO J. 15, 4081-4092.
29. Matzke, M.A., and Matzke, A.J.M. (1995). How and why do plants inactivate homologous (trans)genes? Plant Physiol. 107, 679-685.
30. McIntyre, C.L., Bettenay, H.M., and Manners, J.M. (1996). Strategies for the suppression of peroxidase gene expression in tobacco. II. In vivo suppression of peroxidase activity in transgenic tobacco using ribozyme and antisense constructs. Transgen. Res. 5, 263-270.
31. Milligan, J.F., and Uhlenbeck, O.C. (1989). Synthesis of small RNAs using T7 RNA polymerase. Methods Enzymol. 180, 51-59.
32. Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473-497.
33. Murray, E.E., Buchholz, W.G., and Bowen, B. (1990). Direct analysis of RNA transcripts in electroporated carrot protoplasts. Plant Cell Rep. 10, 129-132.
34. Napoli, C., Lemieux, C., and Jorgensen, R. (1990). Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in frans. Plant Cell 2, 279-289.
35. Pareddy, D., Petolino, J., Skokut, T., Hopkins, N., Miller, M., Welter, M., Smith, K., Clayton, D., Pescitelli, S., and Gould, A. (1997). Maize transformation via helium blasting. Maydica 42, 143-154.
36. Perriman, R., Graf, L., and Gerlach, W.L. (1993). A ribozyme that enhances gene suppression in tobacco protoplasts. Antisense Res. Dev. 3, 253-263.
37. Perriman, R., Bruening, G., Dennis, E.S., and Peacock, W.J. (1995). Effective ribozyme delivery in plant cells. Proc. Natl. Acad. Sci. USA 92, 715-731.
38. Pley, H.W., Flaherty, K.M., and McKay, D.B. (1994). Three-dimensional structure of a hammerhead ribozyme. Nature 372, 68-74.
39. Ruffner, D.E., Stormo, G.D., and Uhlenbeck, O.C. (1990). Sequence requirements of the hammerhead RNA self-cleavage reaction. Biochemistry 29, 10695-10701.
40. Saghai-Maroof, M.A., Soliman, K.M., Jorgenson, R.A., and Allard, R.W. (1984). Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci. USA 81, 8014-8018.
41. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press).
42. Scaringe, S.A., Francklyn, C., and Usman, N. (1990). Chemical synthesis of biologically active oligonucleotides using beta-cyanoethyl protected ribonucleoside phosphoamidites. Nucleic Acids Res. 18, 5433-5441.
43. Schenk, R.V., and Hildebrant, A.C. (1972). Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50, 199-204.
44. Shanklin, J., and Somerville, C.R. (1991). Stearyl-acyl carrier protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs. Proc. Natl. Acad. Sci. USA 88, 2510-2514.
45. Slocombe, S.P., Cummins, I., Jarvis, R.P., and Murphy, D.J. (1992). Nucleotide sequence and temporal regulation of a seed-specific Brassica napus cDNA encoding a stearoyl-acyl carrier protein (ACP) desaturase. Plant Mol. Biol. 20, 151-155.
46. Slocombe, S.P., Piffanelli, P., Fairbairn, D., Bowra, S., Hatzopoulos, P., Tsiantis, M., and Murphy, D.J. (1994). Temporal and tissue-specific regulation of a Brasica napus stearoyl-acyl carrier protein desaturase gene. Plant Physiol. 104, 1167-1176.
47. Smith, C.J., Watson, C.F., Ray, J., Bird, C.R., Morris, P.C., Schuch, W., and Grierson, D. (1988). Antisense RNA inhibition of polygalacturonidase gene expression in transgenic tomatoes. Nature 334, 724-726.
48. Southern, E. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503.
49. Southern, E. (1980). Gel electrophoresis of restriction fragments. Methods Enzymol. 68, 152-176.
50. Steinecke, P., Hergel, T., and Schreier, P.H. (1992). Expression of a chimeric ribozyme gene results in endonucleolytic cleavage of target mRNA and a concommitant reduction of gene expression in vivo. EMBO J. 11, 1525-1530.
51. Symons, R.H. (1994). "Ribozymes." Curr. Opin. Struct. Biol. 4, 322-330.
52. Tabler, M. (1993). Antisense RNA in plants: A tool for analysis and suppression of gene function. In Morphognesis in Plants, K.A. Roubelakis-Angelakis and K. Tran Thanh Van, eds (New York: Plenum Press), pp. 237-258.
53. Thompson, G.A., Scherer, D.E., Foxall-Van Aken, S., Kenny, J.W., Young, H.L., Shintani, D.K., Kridl, J.C., and Knauf, V.C. (1991). Primary structures of the precursor and mature forms of stearoyl-acyl carrier protein desaturase from safflower embryos and requirement of ferredoxin for enzyme activity. Proc. Natl. Acad. Sci. USA 88, 2578-2582.
54. Thompson, J.D., Ayers, D.F., Malstrom, T.A., McKenzie, T.L., Ganousis, L., Chowrira, B., Couture, L., and Stinchcomb, D.T. (1995). Improved accumulation and activity of ribozymes expressed from a tRNA-based RNA polymerase III promoter. Nucleic Acids Res. 23, 2259-2268.
55. Tomizawa, J. (1993). Evolution of functional structures of RNA. In The RNA World, R.F. Gesteland and J.F. Atkins, eds (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press), pp. 419-445.
56. Towbin, H., Staehelin, T., and Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedures and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
57. Uhlenbeck, O.C. (1987). A small catalytic oligoribonucleotide. Nature 328, 596-600.
58. van der Krol, A.R., Lenting, P.E., Veenstra, J., van der Meer, I.M., Koes, R., Gerats, A.G.M., Mol, J.N.M., and Stuitje, A.R. (1988). An antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333, 866-869.
59. van der Krol, A.R., Mur, L.A., Beld, M., Mol, J.N.M., and Stuitje, A.R. (1990). Flavonoid genes in petunia: Addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell 2, 291-299.
60. van Hoof, A., and Green, P.G. (1996). Premature nonsense codons decrease the stability of phytohemagglutinin mRNA in a position dependent manner. Plant J. 10, 415-424.
61. Wegener, D., Steinecke, P., Herget, T., Petereit, I., Philipp, C., and Schreier, P.H. (1994). Expression of a reporter gene is reduced by a ribozyme in transgenic plants. Mol. Gen. Genet. 245, 465-470.
62. Werner, M., and Uhlenbeck, O.C. (1995). The effect of base mismatches in the substrate recognition helices of hammerhead ribozymes on binding and catalysis. Nucleic Acids Res. 23, 2092-2096.
63. Wincott, F.E., DiRenzo, A., Shaffer, C., Grimm, S., Tracz, D., Workman, C., Sweedler, D., Gonzalez, C., Scaringe, S.A., and Usman, N. (1995). Synthesis, deprotection, analysis and purification of RNA and ribozymes. Nucleic Acids Res. 23, 2677-2684.
64. Zaug, A.J., Been, M.D., and Cech, T.R. (1986). The Tetrahymena ribozyme acts like an RNA restriction endonuclease. Nature 324, 429-433.
65. Zuker, M. (1989). On finding all the suboptimal foldings of an RNA molecule. Science 244, 48-52.