Rapid single-tube genotyping of the factor V leiden and prothrombin mutations by real-time PCR using dual-color detection

Clinical Chemistry

Volumn 46, Issue 8 I, 2000, Pages 1191-1195

  Van den Bergh, F A J T M ^a   Van Oeveren Dybicz, A M ^a   Bon, M A M ^a  

^a MEDISCH SPECTRUM TWENTE   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD CLOTTING FACTOR 5 LEIDEN; FLUORESCEIN; MUTANT PROTEIN; PROTHROMBIN;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE; GENETIC POLYMORPHISM; GENOTYPE; HUMAN; HUMAN TISSUE; MELTING POINT; NUCLEOTIDE SEQUENCE; POINT MUTATION; POLYMERASE CHAIN REACTION; THROMBOPHILIA; VEIN THROMBOSIS;

EID: 0033870902     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/46.8.1191     Document Type: Article

References (24)

1. Mutation in blood coagulation factor V associated with resistance to activated protein C
2. Activated protein C resistance as an additional risk factor for thrombosis in protein C-deficient families
3. Detection of the factor V Leiden mutation using whole blood PCR
4. Chemiluminescent detection of factor V Leiden in a routine laboratory
5. Rapid simultaneous screening of factor V Leiden and G20210A prothrombin variant by multiplex polymerase chain reaction on whole blood
6. Simultaneous polymerase chain reaction restriction fragment length polymorphism identification of the factor V Leiden allele and the prothrombin 20210A mutation
7. Simple and rapid combined genetic diagnosis of mutation (1691G→A) of the factor V gene and (20210 G→A) of the prothrombin gene
8. Rapid multiplex analysis for the factor V Leiden and prothrombin G20210A mutations associated with hereditary thrombophilia
9. Two multiplex PCR-based DNA assays for the thrombosis risk factors prothrombin G20210A and coagulation factor V G1691A polymorphisms
10. Development of a simple multiplex polymerase chain reaction for the simultaneous detection of factor V Leiden and prothrombin 20210A mutations
11. Double fluorescent-refractory mutation detection (dF-ARMS) of the factor V Leiden and prothrombin mutations
12. Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR
13. High-speed prothrombin G→A 20210 and methylenetetrahydrofolate reductase C→T 677 mutation detection using real-time fluorescence PCR and melting curves
14. Rapid detection of prothrombotic mutations of prothrombin (G20210A), factor V (G1691A), and methylenetetrahydrofolate reductase (C677T) by real-time fluorescence PCR with the LightCycler
15. Rapid detection of CYP2C9*3 alleles by real-time fluorescence PCR based on SYBR green
16. High-speed apolipoprotein E genotyping and apolipoprotein B3500 mutation detection using real-time fluorescence PCR and melting curves
17. Rapid genotyping of hemochromatosis gene mutations on the LightCycler with fluorescent hybridization probes
18. Color multiplexing hybridization probes using the apolipoprotein E locus as a model system for genotyping
19. A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis
20. Reliable genotyping of the G-20210-A mutation of coagulation factor II (prothrombin)
21. Genotyping of HLA-B27 by real-time PCR without hybridization probes
22. 1-antitrypsin genotyping by multiplex real-time fluorescence PCR with the LightCycler
23. Rapid β-globin genotyping by multiplexing probe melting temperature and color