Probing ligand protein binding equilibria with fluorescence fluctuation spectroscopy

Biophysical Journal

Volumn 79, Issue 2, 2000, Pages 1074-1084

  Chen, Yan ^a   Müller, Joachim D ^a   Tetin, Sergey Y ^b   Tyner, Joan D ^b   Gratton, Enrico ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b ABBOTT DIAGNOSTICS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIGOXIGENIN;

ANALYTIC METHOD; ARTICLE; BINDING SITE; CALCULATION; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; MEASUREMENT; PROTEIN BINDING; STOICHIOMETRY;

EID: 0033869619     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(00)76361-2     Document Type: Article

References (7)

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3. Birks, J. B. 1970. Photophysics of Aromatic Molecules. Wiley Interscience, London and New York.
4. Bonnet, G., O. Krichevsky, and A. Libchaber. 1998. Kinetics of conformational fluctuations in DNA hairpin loops. Proc. Natl. Acad. Sci. USA. 95:8602-8606.
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6. Chen, Y. 1999. Analysis and Applications of Fluorescence Fluctuation Spectroscopy. University of Illinois at Urbana-Champaign, Urbana, IL (available at http://web.physics.uiuc.edu/Research/Fluorescence/index.html).
7. Chen, Y., J. D. Muller, P. T. So, and E. Gratton. 1999. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys. J. 77: 553-567.