Probing the physiological state of phytoplankton at the single-cell level

Scientia Marina

Volumn 64, Issue 2, 2000, Pages 183-195

  Jochem, F J ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Flow cytometry; Fluorescent dyes; Molecular probes; Physiology; Phytoplankton

Indexed keywords

ALKALINE PHOSPHATASE; BIOINDICATOR; CELL FUNCTION; CELL MEMBRANE; CELL SURVIVAL; COPPER; DNA DAMAGE; ELECTRON TRANSFER; FLOW CYTOMETRY; FLUORESCEIN DIACETATE; MARINE ENVIRONMENT; MEMBRANE POTENTIAL; NITRATE REDUCTASE; NITROGEN; PHOTOSYNTHESIS; PHYTOPLANKTON; POLLUTION MONITORING;

FLOW CYTOMETRY; PHYSIOLOGY; PHYTOPLANKTON;

ALGAE; BACILLARIOPHYTA;

EID: 0033866077     PISSN: 02148358     EISSN: None     Source Type: Journal    
DOI: 10.3989/scimar.2000.64n2183     Document Type: Conference Paper

References (100)

1. Response of the marine microalga Dunaliella tertiolecta (Chlorophyceae) to copper toxicity in short time experiments
2. Phytoplankton optical properties: Flow cytometric examination of dilution-induced effects
3. Toxic effects of copper on Selenastrum capricornutum measured by a flow cytometry-based method
4. Brevetoxins and binding: Sodium channels versus antibodies
5. Specific antibodies directed against toxins of Ptychodiscus brevis
6. Algal nitrate reductases
7. Responses to different quantum flux densities
8. Wave-length-dependent induction of thymine dimers and growth rate reduction in the marine diatom Cyclotella sp. exposed to ultraviolet radiation
9. Monitoring ultraviolet-B-induced DNA damage in individual diatom cells by immunofluorescent thymine dimer detection
10. UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp.
11. Functional domains of assimilatory nitrate reductases and nitrite reductases
12. Flow cytometry of acute physiological changes in a marine diatom stressed by copper
13. Toxic action of copper on the membrane system of a marine d iatom measured by flow cytometry
14. Functional analysis of phytoplankton by flow cytometry: A study of the effect of copper on a marine diatom
15. Disorders provoked by copper in the marine diatom Phaeodactylum tricornutum in short-time exposure assays
16. Narrow angle forward light scattering from individual algal cells: Implications for size and shape discrimination in flow cytometry
17. Immunofluorescent reagents: Preparation and analysis
18. Coagulation efficiency, organic matter glues and the dynamics of particles during a phytoplankton bloom in mesocosm study
19. Phototrophic and heterotrophic picoand nanoplankton in anoxic wat ers of the Central Baltic Sea
20. Rapid analytical technique for the assessment of cell metabolic activity in marine microalgae
21. Anomalous behaviour of forward and perpendicular light scattering of cyanobacterium owing to intracellular gas vacuoles
22. Uptake of new and regenerated forms of nitrogen in primary productivity
23. Identification and characterization of a Prorocentrum minimum cell surface protein specifically induced under phosphorus limitation
24. Phosphorus stress detected in cultures and field populations of the dinoflagellate Prorocentrum minimum using a single cell alkaline phosphatase assay
25. Particulate organic matter flux and planktonic new production in the deep ocean
26. Cryopreservation of fluorescent marker-labelled algae (Selenastrum capricornutum) for toxicity testing using flow cytometry
27. Physiological limitations on phytoplankton productivity in the ocean
28. Role of edd y pumping in enhancing primary production in the ocean
29. Immunolocalization of nitrate reductase in the marine dinoflagellate Gonyaulax polyedra (Pyrrophyta)
30. A rapid simple technique utilizing Calcofluor white M2R for the visualization of dinoflagellate thecal plates
31. Evaluation of photosynthetic capacity in phytoplankton by flow cytometric analysis of DCMU-enhanced chlorophyll fluorescence
32. Nitrate reductase from the marine diatom Skeletonema costatum
33. The use of FDA and flow cytometry to measure the metabolic activity of the cyanobacteria
34. Fluorescence assessment of the maximum quantum efficiency of photosynthesis in the western North Atlantic
35. UV damage to plant life in a photobiologically dynamic environment: The case of marine phytoplankton
36. Detection and quantification of alkaline phosphatase in single cells of phosphorus-starved marine phytoplankton
37. Physiological responses of D. tertiolecta to phosphorus limitation
38. Nitrogen limitation of North Atl antic phytoplankton: Analysis of physiological condition in nutrient enrichment experiments
39. Lipolysis of fluorescein and eosin esters - kinetics of hydrolysis
40. Preparation of model haptens to express common epitopes of PSP t oxins
41. FDA hydrolysis and resazurin reduction as a measure of microbial activity in sediments from the south-east Atlantic
42. Use of nuclepore filters for counting bacteria by fluorescence microscopy
43. Sensitive methods for the determination of microbial activities in water sam ples using fluorigenic substrates
44. Autecology of the toxic dinoflagellate Alexandrium ostenfeldii: Life history and growth at different temperatures and salinities
45. On the distribution and importance of picocy-anobacteria in a Boreal inshore area (Kiel Bight, Western Baltic)
46. Distribution and importance of autotrophic ultraplankton in a Boreal inshore area (Kiel Bight, Western Baltic)
47. Dark survival strategies in marine phytoplankton assessed by cytometric measurement of metabolic activity with fluorescein diacetate
48. Cytometric measurement of the DNA cell cycle in the presence of chlorophyll autofluorescence in marine eukaryotic phytoplankton by the blue-light excited dye YoYo-1
49. Cytometric quantification of nitrate reductase by immunolabeling of polyclonal antibodies i n the marine diatom Skeletonema costatum
50. Iron limitation of phytoplankton photosynthesis in the equatorial Pacific Ocean
51. Use of active fluorescence to estimate phytoplankton photosynthesis in situ
52. Natural variability in photosynthetic energy conversion efficiency: A field study in the Gulf of Maine
53. Hydrolytic activities of organisms and biogenic structures in deep-sea sediments
54. Use of a new fluorigenic phosphatase substrate in immunohistochemical applications
55. Flavodoxin as an in situ marker for iron stress in phytoplankton
56. Flavodoxin expression as an indicator of iron limitation in marine diatoms
57. Ultraphytoplankton in the eastern Mediterranean Sea: Towards deriving phytoplankton biomass from flow cytometric measurements of abundance, fluorescence and light scatter
58. Growth characteristics of marine phytoplankton determined by cell cycle proteins: The cell cycle of Ethmodiscus rex (Bacillariophyceae) in the southwestern North Atlantic Ocean and Caribbean Sea
59. Detection of proliferating cell nuclear antigen analog in four species of marine phytoplankton
60. Growth characteristics of phytoplankton determined by cell cycle proteins: PCNA immunostaining of Dunaliella tertiolecta (Chlorophyceae)
61. Regulation of ntcA expression and nitrite uptake in the marine Synechococcus sp. strain WH 7803
62. Iron deficiency limits phytoplankton growth in the north-east Pacific Subarctic
63. Iron in Antarctic waters
64. Inadequacy of Rubisco initial and total activities to account for observed rates of photosynthetic carbon dioxide assimilation by Scenedesmus Ec ornis
65. Effects of darkness on multi-excitation in vivo fluorescence and survival in a marine diatom
66. Phytoplankton photosynthetic characteristics from fluorescence induction assays of individual cells
67. Photosynthetic characteristics of marine phytoplankton from pump-during-probe fluorometry of individual cells at sea
68. Potential of flow cytometry for 'pump and probe' fluorescence measurements of phytoplankton photosynthetic characteristics
69. An immunoprobe to measure Rubisco concentrations and maximal photosynthetic rates in individual phytoplankton cells
70. Optimization of an immunofluorescent assay of the internal enzyme ribulose-1.5-bis-phosphate carboxylase (Rubisco) in single phytoplankton cells
71. Probes for assessing single-cell primary production: Antibodies against ribulose-1.5-bisphosphate carboxylase (RuBPCASE) and peridinin/chlorophyll a protein (PCP)
72. The effect of heavy metals on photosynthesis and los s of cell potassium in two species of marine alga. Dunaliella tertiolecta and Phaeodactylum tricornutum
73. The role of particulate carbohydrate exudates in the flocculation of diatom blooms
74. Semi-automated position analysis using laser scanning microscopy of cells transfected in a regenerating Newt limb
75. Identification of resting cells by dual-parameter flow cytometry of statin expression and DNA content
76. Immuno flow cytometry in marine phytoplankton research
77. Photoadaptation in marine phytoplankton: Variations in ribulose-1,5-bisphosphate activity
78. Detection of cyclobutane thymine dimers in DNA of human cells with monoclonal antibodies raised against a thymine dimer-containing tetranucleotide
79. Copper quenching of the variable fluorescence in Dunaliella tertiolecta. New evidence for a copper inhibition effect of PSII photochemistry
80. A method for studying photosynthetic capacities of unicellular algae based on in vivo fluorescence
81. Heavy-metal induced changes in nonproteinaceous thiol levels and heavy-metal binding peptide in Tetraselmis tetrahele (Prasinophyceae)
82. An immunological approach to detect phosphate stress in populations and single cells of photosynt hetic picoplankton
83. Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter
84. Thymine dimers and inhibition of DNA synthesis by ultraviolet irradiation of cells
85. Role of sinking in diatom life-history cycles: Ecological, evolutionary and geological significance
86. Enzyme immunoassay for the determination of domoic acid in mussel extracts
87. Molecular and physiological response of di atoms to variable levels of irradiance and nitrogen availability: Growth of Skeletonema costatum in simulated upwelling conditions
88. Mechanism of toxicity of ionic copper and copper complexes to algae
89. Flow cytometry instrumentation
90. Algal picoplankton from marine and freshwater ecosystems: A multidisciplinary perspective
91. Selective predation by F avella ehrenbergii (Tintinnia) on and among dinoflagellates
92. Repair of genetic damage induced by UV-B (290-320 nm) radiation
93. Ap plication of flow cytometry in marine phytoplankton research: Current applications and future perspectives
94. Nitrogen transport by vertically migrating diatom mats in the North Pacific Ocean
95. Sugar-containing compounds on the cell surfaces of marin e diatoms measured using concanavalin A and flow cytometry
96. Flow cytometry and cell sorting: A technique for analysis and sorting of aquatic particles
97. Profiting from the visible spectrum
98. Iron-enrichment bottle experiments in the Equatorial Pacific: Responses of individual phytoplankton cells