Measuring cell proliferation in the rectal mucosa: Comparing bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen (PCNA) assays

Journal of Clinical Epidemiology

Volumn 53, Issue 8, 2000, Pages 875-883

  Kulldorff, Martin ^a   McShane, Lisa M ^a   Schatzkin, Arthur ^a   Freedman, Laurence S ^a   Wargovich, Michael J ^b   Woods, Cindy ^b   Purewal, Madhu ^b   Burt, Randall W ^c   Lawson, Michael ^d   Mateski, Donna J ^e   Lanza, Elaine ^a   Corle, Donald K ^a   O'Brien, Barbara ^f   Moler, James ^g  

^a NATIONAL CANCER INSTITUTE   (United States)

^b UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^c UNIVERSITY OF UTAH   (United States)

^d Kaiser Medical Center   (United States)

^e WALTER REED ARMY MEDICAL CENTER   (United States)

^f WESTAT   (United States)

^g INFORMATION MANAGEMENT SERVICES INC   (United States)

Author keywords

Biopsies; Colon adenomas; Colorectal cancer; Correlation; Variance components

Indexed keywords

BROXURIDINE; CYCLINE;

ADULT; ARTICLE; BIOASSAY; BIOPSY; CELL PROLIFERATION; COLON ADENOMA; COLORECTAL CANCER; FEMALE; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; RECTUM MUCOSA; VARIANCE;

ADENOMA; ADULT; ANALYSIS OF VARIANCE; BIOPSY; BROMODEOXYURIDINE; CELL DIVISION; COLORECTAL NEOPLASMS; FEMALE; HUMANS; INTESTINAL MUCOSA; MALE; MIDDLE AGED; PROLIFERATING CELL NUCLEAR ANTIGEN; RECTUM;

EID: 0033865947     PISSN: 08954356     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0895-4356(99)00180-8     Document Type: Article

References (36)

1. Biasco G., Paganelli G.M., Miglioli M.et al. Rectal cell proliferation and colon cancer risk in ulcerative colitis. Cancer Res. 50:1990;1156-1159.
2. Bleiberg H., Buyse M., Galand P. Cell kinetic indicators of premalignant stages of colorectal cancer. Cancer. 56:1985;124-129.
3. Frommel T.O., Mobarhan S., Doria M.et al. Effect of beta-carotine supplementation on indices of colonic cell proliferation. J Natl Cancer Inst. 6:1995;1781-1787.
4. Risio M., Lipkin M., Candelaresi G.et al. Correlation between rectal mucosa cell proliferation and the clinical and pathological features of nonfamilial neoplasia of the large intestine. Cancer Res. 51:1991;1917-1921.
5. Terpstra O.T., Blankenstein M., Dees J.et al. Abnormal pattern of cell proliferation in the entire colonic mucosa of patients with colon adenomas or cancer. Gastroenterology. 92:1987;704-708.
6. Alberts D.S., Einspahr J., Ritenbaugh C.et al. The effect of wheat bran fiber and calcium supplementation on rectal mucosal proliferation rates in patients with resected adenomatous colorectal polyps. Cancer Epidemiol Biomarkers Prev. 6:1997;161-169.
7. Armitage N.C., Rooney P.S., Gifford K.A.et al. The effect of calcium supplements on rectal mucosal proliferation. Br J Cancer. 71:1995;186-190.
8. Baron J.A., Tosteson T.D., Wargovich M.J.et al. Calcium supplementation and rectal mucosal proliferation. a randomized controlled trial J Natl Cancer Inst. 87:1995;1303-1307.
9. Bostick R.M., Fosdick L., Wood J.R.et al. Calcium and colorectal epithelial cell proliferation in sporadic adenoma patients. a randomized, double-blinded, placebo-controlled clinical trial J Natl Cancer Inst. 87:1995;1307-1315.
10. Buset M., Lipkin M., Winawer S.et al. Inhibition of human colonic epithelial cell proliferation in vivo and in vitro by calcium. Cancer Res. 46:1986;5426-5430.
11. Caderni G., Bianchini F., Russo A.et al. Miotic activity in colorectal mucosa of healthy subjects in two Italian areas with different dietary habits. Nutr Cancer. 19:1993;263-268.
12. Einspahr J.G., Alberts D.S., Gapstur S.M.et al. Surrogate end-point biomarkers as measures of colon cancer risk and their use in cancer chemoprevention trials. Cancer Epidemiol Biomarkers Prev. 6:1997;37-48.
13. Lipkin M., Newark H. Effect of added dietary calcium on colonic epithelial-cell proliferation in subjects at high risk for familial colonic cancer. N Engl J Med. 313:1985;1381-1384.
14. Stadler J., Stern H.S., Sing Yeung K.et al. Effect of high fat consumption on cell proliferation activity of colorectal mucosa and on soluble faecal bile acids. Gut. 29:1988;1326-1331.
15. Darmon E., Pincu-Hornstein A., Rozen P. A rapid and simple in vitro method for evaluating human colorectal epithelial proliferation. Arch Pathol Lab Med. 114:1990;855-857.
16. Morstyn G., Pyke K., Gardner J.et al. Immunohistochemical identification of proliferating cells in organ culture using bromodeoxyuridine and a monoclonal antibody. J Histochem Cytochem. 34:1986;697-701.
17. Potten C.S., Kellett M., Roberts S.A.et al. Measurement of in vivo proliferation in human colorectal mucosa using bromodeoxyuridine. Gut. 33:1992;71-78.
18. Roe D.J., Alberts D.S., Wargovich M.J.et al. Reproducibility of the measurement of colonic proliferation using bromodeoxyuridine across laboratories. Cancer Epidemiol Biomarkers Prev. 5:1986;349-353.
19. Kubben F.J., Peeters-Haesevoets A., Engels L.G.et al. Proliferating cell nuclear antigen (PCNA). a new marker to study human colonic cell proliferation Gut. 35:1994;530-535.
20. Takasaki Y., Deng J.S., Tan E.M. A nuclear antigen associated with cell proliferation and blast transformation. J Exp Med. 154:1981;1899-1909.
21. Tanaka K., Murata N., Yanai H., Okita K. Immunohistological study on the expression of proliferating cell nuclear antigen (PCNA/cyclin) in human colorectal lesions. Nippon Shokakibyo Gakkai Zasshi. 89:1992;493-497.
22. Kurki P., Ogata K., Tan E.M. Monoclonal antibodies to proliferating cell nuclear antigen (PCNA/cyclin) as probes for proliferating cells by immunofluorescence microscopy and flow cytometry. J Immunol Methods. 109:1988;49-59.
23. Richter F., Richter A., Yang K., Lipkin M. Cell proliferation in rat colon measured with bromodeoxyuridine, proliferating cell nuclear antigen, and [H3]thymidine. Cancer Epidemiol Biomarkers Prev. 1:1992;561-566.
24. Bostick R.M., Fosdick L., Lillemoe T.J.et al. Methodological findings and considerations in measuring colorectal epithelial cell proliferation in humans. Cancer Epidemiol Biomarkers Prev. 6:1997;931-942.
25. Bromley M., Rew D., Becciolini A.et al. A comparison of proliferation markers (BrdUrd, Ki-67, PCNA) determined at each cell position in the crypts of normal human colonic mucosa. Eur J Histochem. 40:1996;89-100.
26. Kilias D., Macrae F.A., Sharpe K., Young G.P. The effect of incubation of rectal biopsies on measures of proliferation using proliferating cell nuclear antigen in comparison with 5-bromo-2-deoxyuridine. Cancer Epidemiol Biomarkers Prev. 6:1997;819-824.
27. Risio M., Candelaresi G., Rossini F.P. Bromodeoxyuridine uptake and proliferating cell nuclear antigen expression throughout the colorectal tumor sequence. Cancer Epidemiol Biomarkers Prev. 2:1993;363-367.
28. Weisgerber U.M., Boeing H., Nemitz R.et al. Proliferation cell nuclear antigen (clone 19A2) correlates with 5-bromo-2-deoxyurdine labeling in human colonic epithelium. Gut. 34:1993;1587-1592. , 1994;35:717-8.
29. Schatzkin A., Lanza E., Freedman L.S.et al. The polyp prevention trial. I. Rationale, design, recruitment, and baseline participant characteristics Cancer Epidemiol Biomarkers Prev. 5:1996;375-383.
30. McShane L.M., Kulldorff M., Wargovich M.J.et al. An evaluation of rectal mucosal proliferation measure variability sources in the polyp prevention trial. can we detect informative differences among individuals' proliferation measures amid the noise? Cancer Epidemiol Biomarkers Prev. 7:1998;605-612.
31. Spearman C. The proof and measurement of association between two things. Am J Psychol. 15:1904;72-101.
32. Yule G.U. On the significance of Bravais' formulae for regression in the case of skew correlation. Proc R Soc Lond B. 60:1897;477-489.
33. SAS v 6.12, SAS Institute Inc., SAS Circle, Box 8000, Cary NC 27512-8000, USA.
34. Searle S.R., Casella G., McCulloch C.E. Variance Components. 1992;Wiley, New York.
35. Lin L.I. A concordance correlation coefficient to evaluate reproducibility. Biometrics. 45:1989;255-268.
36. Wolfinger R. A tutorial on the mixed models. In: Cary, NC: Getting Started With PROC MIXED. Cary, NC: SAS Institute Inc., 1992.