NTP binding and phosphohydrolase activity associated with purified bluetongue virus non-structural protein NS2

Journal of General Virology

Volumn 81, Issue 8, 2000, Pages 1961-1965

  Horscroft, N J ^a   Roy, P ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; CALCIUM ION; GUANOSINE TRIPHOSPHATE; MAGNESIUM ION; NS 2 PROTEIN; NUCLEOSIDE TRIPHOSPHATE; OUABAIN; PHOSPHATASE; PHOSPHOPROTEIN; RECOMBINANT PROTEIN; RNA BINDING PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ARTICLE; BACULOVIRUS; BINDING AFFINITY; BLUETONGUE ORBIVIRUS; CELL INCLUSION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; HYDROLYSIS; INSECT; NONHUMAN; PHOSPHATE METABOLISM; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

BLUETONGUE VIRUS; INSECTA; RNA VIRUSES; STROPHANTHUS GRATUS; UNIDENTIFIED BACULOVIRUS;

EID: 0033865718     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-81-8-1961     Document Type: Article

References (32)

1. Recovery and characterization of a replicase complex in rotavirus-infected cells by using a monoclonal antibody against NSP2
2. Characterization of the nucleoside triphosphate phosphohydrolase and helicase activities of the reovirus lambda-1 protein
3. Presence of nucleoside triphosphate phosphohydrolase activity in purified virions of reovirus
4. Characterization of virus inclusion bodies in bluetongue virus-infected cells
5. Protein P4 of the bacteriophage ∅6 procapsid has a nucleoside triphosphate-binding site with associated nucleoside triphosphate phosphohydrolase activity
6. Orbivirus non-structural protein NS2: Its role in virus replication
7. In vitro phosphorylation and purification of a nonstructural protein of bluetongue virus with affinity for single-stranded RNA
8. The rotavirus nonstructural protein, NS35, possesses RNA-binding activity in vitro and in vivo
9. The rotavirus RNA-binding protein NS35 (NSP2) forms 10S multimers and interacts with the viral RNA polymerase
10. Rotavirus VP3 expressed in insect cells possesses guanylyltransferase activity
11. Characterization of the nucleoside triphosphate phosphohydrolase (ATPase) activity of RNA synthesis termination factor p. I. Enzymatic properties and effects of inhibitors
12. Characterization of the nucleoside triphosphate phosphohydrolase (ATPase) activity of RNA synthesis termination factor p. II. Influence of synthetic RNA homopolymers and random copolymers on the reaction
13. Guanylyltransferase and RNA 5'-triphosphatase activities of the purified expressed VP4 protein of bluetongue virus
14. Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode
15. Double-stranded RNA bacteriophage ∅6 protein P4 is an unspecific nucleoside triphosphatase activated by calcium ions
16. RAN/TC4 mutants identify a common requirement for snRNP and protein import into the nucleus
17. Characterization of rotavirus guanylyltransferase activity associated with polypeptide VP3
18. In vivo and in vitro phosphorylation of rotavirus NSP5 correlates with its localization in viroplasms
19. Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome
20. Structure of the bluetongue virus genome and its encoded proteins
21. A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis
22. A dominant negative mutation in a spliceosomal ATPase affects ATP hydrolysis but not binding to the spliceosome
23. Bluetongue virus VP6 binds ATP and exhibits an RNA-dependent ATPase function and a helicase activity that catalyze the unwinding of double-stranded RNA substrates
24. Multimers formed by the rotavirus nonstructural protein NSP2 bind to RNA and have nucleoside triphosphatase activity
25. Comparison of the expression and phosphorylation of the non-structural protein NS2 of three different orbiviruses: Evidence that phosphorylation involves an ubiquitous cellular kinase
26. Synthesis of bluetongue virus-encoded phosphoprotein and formation of inclusion bodies by recombinant baculovirus in insect cells
27. The multimeric nonstructural NS2 proteins of bluetongue virus, African horsesickness virus, and epizootic hemorrhagic disease virus differ in their single-strand RNA-binding ability
28. Studies on the in vitro and the in vivo transcription of the bluetongue virus genome
29. Deletion and mutational analyses of bluetongue virus NS2 protein indicate that the amino but not the carboxy terminus of the protein is critical for RNA-protein interactions