Field emission scanning electron microscopy (FESEM) entering the 21st century: Nanometer resolution and molecular topography of cell structure

Journal of Histotechnology

Volumn 23, Issue 3, 2000, Pages 249-259

  Erlandsen, S L ^a   Frethem, C ^a   Chen, Y ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

Backscatter electron imaging; CryoSEM; Field emission SEM; High resolution SEM; Secondary electron imaging

Indexed keywords

TUNGSTEN;

ARTICLE; CELL SHAPE; CELL STRUCTURE; FILM; IMAGE QUALITY; IMAGING SYSTEM; IMMUNOGOLD STAINING; SCANNING ELECTRON MICROSCOPY; TISSUE PREPARATION; TOPOGRAPHY;

EID: 0033859330     PISSN: 01478885     EISSN: None     Source Type: Journal    
DOI: 10.1179/his.2000.23.3.249     Document Type: Article

References (54)

1. Low voltage scanning electron microscopy
2. The development of field-emission scanning electron microscopy for imaging biological surfaces
3. Low voltage scanning electron microscopy
4. Instrumentation for ultra high resolution scanning electron microscopy
5. The case for low voltage high resolution scanning electron microscopy of biological samples
6. Early results using high-resolution, low-voltage, low-temperature SEM
7. High resolution immunogold localization of Giardia cyst wall antigens using field emission SEM with secondary and backscatter electron imaging
8. Single crystal detector suitable for high resolution scanning electron microscopy
9. Technique for the preservation of three-dimensional structure in preparing specimens for the electron microscope
10. Ligand-mediated osmium binding: Its application in coating biological specimens for SEM
11. 2 integrin (Mac-1) and L-selectin (LECAM-1) adherence receptors on human neutrophils using high resolution field emission SEM
12. Formation of the Giardia Cyst Wall: Studies on Extracellular Assembly using Immunogold Labeling and High Resolution Field Emission SEM
13. Preparative methods for scanning electron microscopy, Chapter 123
14. Clusters of serpentine receptors for human neutrophil chemoattractants IL-8 and C5a
15. Preservation of leukocyte surface molecules by aldehyde fixation: Flow cytometry and high resolution FESEM studies of CD62L, CD11b, and Thy-1
16. High-resolution visualization by field emission scanning electron microscopy of Enterococcus faecalis cell surface proteins encoded by the pheromone-induced conjugative plasmid pCF10
17. Imaging of cytoskeletal elements by low temperature high resolution scanning electron microscopy
18. High-resolution cryo-scanning electron microscopy study of the macromolecular structure of fibronectin fibrils
19. High resolution backscatter electron detection of cell surface molecules on human platelets using the double layer coating method and cryo field emission scanning electron microscopy
20. Double layer coating for high resolution low temperature SEM
21. Non-coating techniques to render biological specimens conductive/1980 update
22. Working at higher magnifications in scanning electron microscopy with secondary and backscattered electrons on metal coated biological specimens and imaging macromolecular cell membrane structures
23. Rationale for the application of thin, continuous metal films in high magnification electron microscopy
24. A revised method for non-coated SEM specimens
25. Application of the OTOTO non-coating technique: A comparison of LM, TEM, and SEM
26. Demonstration of the fine structure of stereocilia in the organ of Corti of the guinea pig by field emission scanning electron microscopy
27. High-resolution field-emission scanning electron microscopy of nuclear pore complex
28. Tannin-ferrocyanide-osmium method for scanning electron microscopy with use of microwave irradiation
29. Application of an ultrahigh-resolution scanning electron microscope (UHS-T1) to biological specimens
30. High resolution scanning electron microscopy in biology
31. Colloidal gold granules as markers for cell surface receptors in the scanning electron microscope
32. Stability and steric hindrance of lectin-labeled gold markers in transmission and scanning electron microscopy
33. Imaging colloidal gold labels in LVSEM
34. Observations of colloidal gold labeled platelet microtubules: High voltage electron microscopy and low voltage-high resolution scanning electron microscopy
35. Detection of protein A gold 15 nm marked surface antigens by backscattered electrons
36. Backscatter electron imaging of the colloidal gold marker on cell surfaces
37. High resolution biological scanning electron microscopy: A comparative study of low temperature metal coating techniques
38. Workshop on high-resolution immunocytochemistry of cell surfaces using field emission SEM
39. High resolution backscatter electron (BSE) imaging of immunogold with in-lens and below-the-lens field emission scanning electron microscopes
40. Freeze fracturing in ultrahigh vacuum at - 196°C
41. Prerequisites of high resolution scanning electron microscopy
42. Progress in scanning electron microscopy of frozen-hydrated biological specimens
43. Double-axis rotary shadowing for high-resolution scanning electron microscopy
44. High-resolution SEM of biological macromolecular complexes
45. Macromolecular imaging of cytoskeletal elements and reovirus by ultra-high resolution cryoscanning electron microscopy
46. Visualization of individual reovirus particles by low-temperature, high-resolution scanning electron microscopy
47. High pressure freezing
48. Advances in ultrarapid freezing for the preservation of cellular ultrastructure
49. Cryofixation without pretreatment at ambient pressure
50. Contrast in high-resolution scanning electron microscope images
51. Limits of SEM resolution
52. Beam interactions, contrast and resolution in the SEM
53. High-resolution scanning electron microscopy
54. Double-layer coating for field-emission cryo-scanning electron microscopy - Present state and applications