Double-layer coating for field-emission cryo-scanning electron microscopy - Present state and applications

Scanning

Volumn 19, Issue 5, 1997, Pages 343-348

  Walther, P ^a   Muller M ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Cryo SEM; Double layer coating; Field emission; Freeze fracturing; High pressure freezing

Indexed keywords

CARBON; PLATINUM;

CONFERENCE PAPER; ELECTRON; FREEZING; IMAGING; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;

EID: 2642653226     PISSN: 01610457     EISSN: None     Source Type: Journal    
DOI: 10.1002/sca.4950190501     Document Type: Conference Paper

References (12)

1. Autrata R, Hermann R, Müller M: An efficient single crystal BSE detector in SEM. Scanning 14, 127-135 (1992)
2. Moor H: Theory and practice of high pressure freezing. In Cryotechniques in Biological Electron Microscopy (Eds. Steinbrecht RA, Zierold K). Springer Verlag, Berlin (1987) L75-191
3. Moor H, Mühlethaler K: Fine structure in frozen-etched yeast cells. J Cell Biol 17, 609-628 (1963)
4. Roof DJ, Heuser JE: Surfaces of rod photoreceptor disk membranes: Integral membrane components. J Cell Biol 95, 487-500 (1982)
5. Sjöstrand FS, Kreman K: Molecular structure of outer segment disks in photoreceptor cells. J Ultrastruct Res 65, 195-226 (1978)
6. Steere RL: Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol 3, 45-60 (1957)
7. Studer D, Michel M, Müller M: High pressure freezing comes of age. Scan Microsc 3(suppl 3), 253-269 (1989)
8. Szczesny PJ, Walther P, Müller M: Light damage in rod outer segments: The effects of fixation on ultrastructural alterations. Curr Eye Res 15, 807-814 (1996)
9. Walther P, Hentschel J: Improved representation of cell surface structures by freeze substitution and backscattered electron imaging. Scan Microsc 3(suppl 3), 201-211 (1989)
10. Walther P, Chen Y, Malecki M, Steffen Zoran SL, Schatten GP, Pawley JB: Scanning electron microscopy of high pressure frozen sea urchin embryos. Scan Microsc 7, 1283-1293 (1993)
11. Walther P, Wehrli E, Hermann R, Müller M: Double layer coating for high resolution low temperature SEM. J Microsc 179, 229-237 (1995)
12. Walther P, Müller M: Low temperature field emission scanning electron microscopy of high pressure frozen samples reveals structural information below 10 nm. Proceedings of Microscopy and Microanalysis. San Francisco Press (1996) 826