Dynamic regulation of mucus gel thickness in rat duodenum

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 279, Issue 2 42-2, 2000, Pages

  Akiba, Yasutada ^a,b   Guth, Paul H ^a   Engel, Eli ^a   Nastaskin, Igor ^a   Kaunitz, Jonathan D ^a,b,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c VA GREATER LOS ANGELES HEALTHCARE SYSTEM   (United States)

Author keywords

Alcian blue staining; Brunner's glands; Fluorescent microspheres; Indomethacin; Perfusate glycoprotein concentration; Periodic acid Schiff staining

Indexed keywords

ACID; ALCIAN BLUE; GLYCOPROTEIN; MICROSPHERE; PROSTAGLANDIN E2; PROSTAGLANDIN SYNTHASE; URETHAN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRUNNER GLAND; CONTROLLED STUDY; DUODENUM; ENZYME INHIBITION; GEL; GOBLET CELL; INTESTINE MUCOSA; MALE; MUCUS SECRETION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; RAT; REGULATORY MECHANISM; THICKNESS;

EID: 0033858103     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.2000.279.2.g437     Document Type: Article

References (48)

1. Higher proximal duodenal mucosal bicarbonate secretion is independent of Brunner's glands in rats and rabbits
2. Acid-sensing pathways of rat duodenum
3. Regulation of intracellular pH and blood flow in rat duodenal epithelium in vivo
4. Adherent and soluble mucus in the stomach and duodenum
5. 2 on gastric mucus gel thickness
6. 2 and carbenoxolone
7. Nitric oxide donors increase mucus gel thickness in rat stomach
8. Interleukin-1 is a mucus secretagogue
9. Demonstration of a pH gradient at the luminal surface of rat duodenum in vivo and its dependence on mucosal alkaline secretion
10. Intravenous injection of guanylin induces mucus secretion from goblet cells in rat duodenal crypts
11. Dot blot analysis of rat gastric mucin using histochemical staining methods
12. Expression cloning of gastric mucin complementary DNA and localization of mucin gene expression
13. Effect of orogastric nicotine on rat gastric mucosal gel thickness, surface cell viability, and intracellular pH
14. A simple method for measuring thickness of the mucus gel layer adherent to rat, frog and human gastric mucosa: Influence to feeding, prostaglandin, N-acetylcysteine and other agents
15. Vasoactive intestinal polypeptidergic nerves and Brunner's gland secretion in the rat
16. Effect of secretin and glucagon on Brunner's gland secretion in the rat
17. Improved sensitivity for detection and quantitation of glycoproteins on polyacrylamide gels
18. Mucus glycoprotein secretion by duodenal mucosa in response to luminal arachidonic acid
19. Mechanisms of the secretory and motor responses of the Brunner's gland region of the intestines to duodenal acidification
20. 2 analog, on human gastric acid secretion and soluble mucin output
21. Deranged hydrophobic barrier of the rat gastroduodenal mucosa after parenteral nonsteroidal anti-inflammatory drugs
22. A colorimetric assay for glycoproteins based on the periodic acid/Schiff stain
23. In vitro measurement of the pH gradient and thickness of the duodenal mucus gel layer in rats
24. Gastric and duodenal surface mucus gel thickness in rat: Effects of prostaglandins and damaging agents
25. Histochemical changes in mucus in duodenal ulceration
26. Pentagastrin enhances gastric mucosal defenses in vivo: Luminal acid-dependent and independent effects
27. Alternating laminated array of two types of mucin in the human gastric surface mucous layer
28. Both crypt and villus intestinal goblet cells secrete mucin in response to cholinergic stimulation
29. Signal transduction pathways mediating mucin secretion from intestinal goblet cells
30. Stimulation by carbachol of mucus gel thickness in rat stomach involves nitric oxide
31. Mucus gel thickness and turnover in the gastrointestinal tract of the rat: Response to cholinergic stimulus and implication for mucoadhesion
32. Interaction between prostanoids, NO, and VIP in modulation of duodenal alkaline secretion and motility
33. Mucus and alkali secretion in the rat duodenum: Effects of indomethacin, N(w)-nitro-L-arginine, and luminal acid
34. Gastroduodenal mucus gel thickness in patients with Helicobacter pylori: A method for assessment of biopsy specimens
35. Molecular anatomy of an endodermal gland: Investigations on mucus glycoproteins and cell turnover in Brunner's glands of Didelphis virginiana using lectins and PCNA immunoreactivity
36. Misoprostol-induced increases in adherent gastric mucus thickness and luminal mucus output
37. Isolation and partial characterization of rat duodenal-gland (Brunner's-gland) mucus glycoprotein
38. Pathogenesis of nonsteroidal anti-inflammatory drug-related upper gastrointestinal toxicity
39. Mechanism of rapid mucus secretion in goblet cells stimulated by acetylcholine
40. Regulation of intestinal goblet cell secretion. I. Role of parasympathetic stimulation
41. Bradykinin modulates mucin secretion but not synthesis from an intestinal goblet cell line
42. Periodic acid/Schiff staining of glycoproteins immobilized on a blotting matrix
43. Role of capsaicin-sensitive afferent neurons in alkaline secretory response to luminal acid in the rat duodenum
44. - secretion in anesthetized rats: Effects of 16,16-dimethyl PGE(2,) topical acid, and acetazolamide
45. 2 receptor activation but not acid secretion
46. Indomethacin does not alter the effect of pentagastrin on rat gastric defense mechanisms
47. Biosynthesis of rat MUC2 in colon and its analogy with human MUC2
48. Gastrointestinal expression and partial cDNA cloning of murine Muc2