The secreted effector protein of Salmonella dublin, SopA, is translocated into eukaryotic cells and influences the induction of enteritis

Cellular Microbiology

Volumn 2, Issue 4, 2000, Pages 293-303

  Wood, Michael W ^a   Jones, Michael A ^a   Watson, Patricia R ^a   Siber, Andrew M ^b   McCormick, Beth A ^b   Hedges, Samantha ^a   Rosqvist, Roland ^c   Wallis, Tim S ^a   Galyov, Edouard E ^a  

^a INSTITUTE FOR ANIMAL HEALTH   (United Kingdom)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c UMEÅ UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; CATTLE; CONTROLLED STUDY; ENTERITIS; GENE MUTATION; HUMAN; HUMAN CELL; INFLAMMATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SALMONELLA DUBLIN;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; CATTLE; CELL LINE; ENTERITIS; EUKARYOTIC CELLS; HELA CELLS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; MUTATION; SALMONELLA; SEQUENCE ALIGNMENT; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); BOS TAURUS; EUKARYOTA; SALMONELLA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR DUBLIN;

EID: 0033858046     PISSN: 14625814     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-5822.2000.00054.x     Document Type: Article

References (28)

1. Cornelis, G.R., and Wolf-Watz, H. (1997) The Yersinia Yop virulon: a bacterial system for subverting eukaryotic cells. Mol Microbiol 23: 861-867.
2. Eckmann, L., Kagnoff, M.F., and Fierer, J. (1993) Epithelial cells secrete the interleukin-8 in response to bacterial entry. Infect Immun 61: 4569-4574.
3. Galán, J.E. (1999) Interaction of Salmonella with host cells through the centisome 63 type III secretion system. Curr Opin Microbiol 2: 46-50.
4. Galyov, E.E., Wood, M.W., Rosqvist, R., Mullan, P.B., Watson, P.R., Hedges, S., et al. (1997) A secreted effector protein of Salmonella dublin is translocated into eukaryotic cells and mediates inflammation and fluid secretion in infected ileal mucosa. Mol Microbiol 25: 903-912.
5. Hardt, W.-D., and Galán, J.E. (1997) A secreted Salmonella protein with homology to a virulence determinant of plant pathogenic bacteria. Proc Natl Acad Sci USA 94: 9887-9892.
6. Heinzinger, N.K., Fujimoto, S.Y., Clark, M.A., Moreno, M.S., and Barrett, E.L. (1995) Sequence analysis of the phs operon in Salmonella typhimurium and the contribution of thiosulfate reduction to anaerobic energy metabolism. J Bacteriol 177: 2813-2820.
7. Henson, P., and Oades, Z.G. (1975) Stimulation of human neutrophils by soluble and insoluble immunoglobulin aggregates. J Clin Invest 56: 1053-1061.
8. Hueck, C.J. (1998) Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev 62: 379-433.
9. Jones, M.A., Wood, M.W., Mullan, P.B., Watson, P.R., Wallis, T.S., and Galyov, E.E. (1998) Secreted effector proteins of Salmonella dublin act in concert to induce enteritis. Infect Immun 66: 5799-5804.
10. Kaniga, K., Uralil, J., Bliska, J.B., and Galán, J.E. (1996) A secreted tyrosine phosphatase with modular effector domains encoded by the bacterial pathogen Salmonella typhimurium. Mol Microbiol 21: 633-641.
11. McCormick, B.A., Colgan, S.P., Delp-Archer, C., Miller, S.I., and Madara, J.L. (1993) Salmonella typhimurium attachment to human epithelia monolayers: transcellular signaling neutrophils. J Cell Biol 123: 895-907.
12. McCormick, B.A., Hofman, P.M., Kim, J., Carnes, D., Miller, S.I., and Madara, J.L. (1995a) Surface attachment of Salmonella typhimurium to intestinal epithelia imprints the subepithelial matrix gradients chemotactic for neutrophils. J Cell Biol 131: 1599-1608.
13. McCormick, B.A., Miller, S.I., Carnes, D., and Madara, J.L. (1995b) Transepithelial signaling to neutrophils by salmonellae: a novel virulence mechanism for gastroenteritis. Infect Immun 63: 2302-2309.
14. McCormick, B.A., Parkos, C.A., Colgan, S.P., Carnes, D.K., and Madara, J.L. (1998) Apical secretion of a pathogen-elicited epithelial chemoattractant (PEEC) activity in respose to surface colonization of intestinal epithelia by Salmonella typhimurium. J Immunol 160: 455-466.
15. Madara, J.L., Colgan, S.P., Nusrat, A., Delp, C., and Parkos, C.A. (1992) A simple approach to measurement of electrical parameters of cultural epithelial monolayers: use in assessing neutrophil epithelial interactions. J Tissue Culture Methods 14: 209.
16. Milton, D.L., O'Toole, R., Hörstedt, P., and Wolf-Watz, H. (1996) Flagellin A is essential for the virulence of Vibrio anguillarum. J Bacteriol 178: 1310-1319.
17. Parkos, C.A., Delp, C., Arnaout, M.A., and Madara, J.L. (1991) Neutrophil migration across a cultured intestinal epithelium: dependence on a CD11b/CD18 mediated event and enhanced efficiency in the physiological direction. J Clin Invest 88: 1605-1612.
18. Rozengurt, E., Legg, A., Strang, G., and Courtenay-Luck, N. (1981) Cyclic AMP: a mitogenic signal for Swiss 3T3 cells. Proc Natl Acad Sci USA 78: 4392-4396.
19. Rout, W.R., Formal, S.B., Dammin, G.J., and Giannella, R.A. (1974) Pathophysiology of Salmonella diarrhea in the Rhesus monkey: intestinal transport, morphological and bacteriological studies. Gastroenterology 67: 59-70.
20. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
21. Simon, R., Preifer, U., and Puhler, A. (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Bio/Technology 1: 784-791.
22. Turnbull, P.C.B., and Richmond, J.E. (1978) A model of Salmonella enteritis: the behaviour of Salmonella enteritidis in the chick intestine studied by light and electron microscopy. Br J Exp Pathol 59: 64-75.
23. Wallis, T.S., Hawker, R.J.H., Candy, D.C.A., Qi, G.-M., Clarke, G.J., Worton, K.J., et al. (1989) Quantification of the leukocyte influx into rabbit ileal loops induced by strains of Salmonella typhimurium of different virulence. J Med Microbiol 30: 149-156.
24. Wallis, T.S., Paulin, S.M., Plested, J.S., Watson, P.R., and Jones, P.W. (1995) The Salmonella dublin virulence plasmid mediates systemic but not enteric phases of salmonellosis in cattle. Infect Immun 63: 2755-2761.
25. Watson, P.R., Paulin, S.M., Bland, A.P., Jones, P.W., and Wallis, T.S. (1995) Characterization of intestinal invasion by Salmonella typhimurium and Salmonella dublin and effect of a mutation in the invH gene. Infect Immun 63: 2743-2754.
26. Watson, P.R., Galyov, E.E., Paulin, S.M., Jones, P.W., and Wallis, T.S. (1998) Mutation of invH, but not stn, reduces Salmonella-induced enteritis in cattle. Infect Immun 66: 1432-1438.
27. Wood, M.W., Rosqvist, R., Mullan, P.B., Edwards, M.H., and Galyov, E.E. (1996) SopE, a secreted protein of Salmonella dublin, is translocated into the target eukaryotic cell via a sip-dependent mechanism and promotes bacterial entry. Mol Microbiol 22: 327-338.
28. Wood, M.W., Jones, M.A., Watson, P.R., Hedges, S., Wallis, T.S., and Galyov, E.E. (1998) Identification of a pathogenicity island required for Salmonella enteropathogenicity. Mol Microbiol 29: 883-892.