Forskolin-induce apical membrane insertion of virally expressed, epitope-tagged CFTR in polarized MDCK cells

American Journal of Physiology - Cell Physiology

Volumn 279, Issue 2 48-2, 2000, Pages

  Howard, Marybeth ^a   Jiang, Xiaosui ^a   Stolz, Donna Beer ^a   Hill, Warren G ^a   Johnson, Jennifer A ^a   Watkins, Simon C ^a   Frizzell, Raymond A ^a   Bruton, Christina M ^b   Robbins, Paul D ^b   Weisz, Ora A ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

Adenovirus; Cystic fibrosis; Cystic fibrosis transmembrane conductance regulator; Epithelia; Madin Darby canine kidney; Protein traffic

Indexed keywords

CYCLIC AMP; EPITOPE; FORSKOLIN; TRANSMEMBRANE CONDUCTANCE REGULATOR; CHLORIDE CHANNEL;

ADENOVIRUS; ANIMAL CELL; ANTIBODY LABELING; APICAL MEMBRANE; ARTICLE; CERCOPITHECIDAE; CONTROLLED STUDY; KIDNEY CELL CULTURE; MEMBRANE STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; ANIMAL; CELL CULTURE; CYTOLOGY; DOG; DRUG EFFECT; KIDNEY; METABOLISM; VIROLOGY;

ADENOVIRIDAE; ANIMALS; CELLS, CULTURED; CHLORIDE CHANNELS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; DOGS; EPITOPES; FORSKOLIN; KIDNEY;

EID: 0033852384     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.2000.279.2.c375     Document Type: Article

References (35)

1. Regulation of plasma membrane recycling by CFTR
2. Cyclic AMP modulates the rate of 'constitutive' exocytosis of apical membrane proteins in Madin-Darby canine kidney cells
3. + pumps in turtle-bladder luminal membrane: Role of cell pH and calcium
4. Mutations in the cystic fibrosis gene in men with congenital bilateral absence of the vas deferens
5. - channels by protein kinase A corrected by insertion of CFTR
6. Cystic fibrosis heterozygote resistance to cholera toxin in the cystic fibrosis mouse model
7. Antidiuretic hormone moves membranes
8. Gsα stimulates transcytosis and apical secretion in MDCK cells through cAMP and protein kinase A
9. Two strains of the Madin-Darby canine kidney (MDCK) cell line have distinct glycosphingolipid compositions
10. Construction of adenovirus vectors through Cre-lox recombination
11. Selective perturbation of apical membrane traffic by expression of influenza virus M2, an acid-activated ion channel, in polarized Madin-Darby canine kidney cells
12. +,K)-ATPase membranes from resting and stimulated gastric parietal cells
13. Epitope tagging permits cell surface detection of functional CFTR
14. cAMP-Regulated trafficking of epitope-tagged CFTR
15. The role of exocytosis in the activation of the chloride conductance in Chinese hamster ovary cells (CHO) stably expressing CFTR
16. Regulation of epithelial sodium channels by the cystic fibrosis transmembrane conductance regulator
17. Glycosylation differences between a cystic fibrosis and rescued airway cell line are not CFTR dependent
18. Interaction between cystic fibrosis transmembrane conductance regulator and outwardiy rectified chloride channels
19. - secretion via CFTR in Calu-3 airway epithelial cells
20. Functional expression and apical localization of the cystic fibrosis transmembrane conductance regulator in MDCK I cells
21. Hyper-expression of recombinant CFTR in heterologous cells alters its physiological properties
22. + reabsorption in A6 cells
23. Membrane trafficking of the cystic fibrosis gene product, cystic fibrosis transmembrane conductance regulator, tagged with green fluorescent protein in Madin-Darby canine kidney cells
24. Regulation of CFTR chloride channels by syntaxin and Munc18 isoforms
25. Syntaxin 1A inhibits CFTR chloride channels by means of domain-specific protein-protein interactions
26. Cystic fibrosis transmembrane conductance regulator-associated ATP and adenosine 3'-phosphate 5'-phosphosulfate channels in endoplasmic reticulum and plasma membrane
27. Activators of protein kinase A stimulate apical but not basolateral transport in epithelial Madin-Darby canine kidney cells
28. Syntaxin 1A inhibits regulated CFTR trafficking
29. Identification of two strains of MDCK cells which resemble separate nephron tubule segments
30. Novel subunit composition of a renal epithelial K(ATP) channel
31. FLAG epitope positioned in an external loop preserves normal biophysical properties of CFTR
32. - channels
33. CFTR as a cAMP-dependent regulator of sodium channels
34. Evidence that insulin causes translocation of glucose transport activity to the plasma membrane from an intracellular storage site
35. CFTR-dependent membrane insertion is linked to stimulation of the CFTR chloride conductance