Three-dimensional light microscopy: Observation of thick objects

Journal of Histotechnology

Volumn 23, Issue 3, 2000, Pages 205-217

  Turner, J N ^a   Shain, W ^a   Szarowski, D H ^a   Lasek, S ^a   Dowell, N ^a   Sipple, B ^a   Can, A ^a   Al Kofahi, K ^a   Roysam, B ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

Confocal microscopy; Depth of field; In focus image; Out of focus light; Photomultiplier tube; Point spread function

Indexed keywords

ARTICLE; CONFOCAL MICROSCOPY; DEVICE; ILLUMINATION; IMAGE ANALYSIS; IMAGE QUALITY; INTERMETHOD COMPARISON; THICKNESS; THREE DIMENSIONAL IMAGING; TISSUE SLICE;

EID: 0033851960     PISSN: 01478885     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Three-dimensional architecture of a polytene nucleus
2. Light microscopic images reconstructed by maximum likelihood deconvolution
3. Automated image analysis technologies for biological 3D light microscopy
4. Cerebral astrocyte response to micro-machined silicon implants
5. Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains
6. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in douvle immunostaining
7. Subcellular localization of specific mRNAs and their protein products in Purkinje cells by combined fluorescence in situ hybridization and immunocytochemistry
8. Confocal light microscopy of brain cells and tissue: Image analysis and quantitation
9. Automated tracing and volume measurements of neurons from 3D confocal fluorescence microscopy data
10. Rapid automated three-dimensional tracing of neurons from confocal image stacks
11. Confocal microscopy and three-dimensional reconstruction of electrophysiologically identified neurons in thick brain slices
12. Three-dimensional imaging and image analysis of hippocampal neurons: Confocal and digitally enhanced wide field microscopy
13. Ultrastructural investigation of neurons identified and localized using the confocal scanning laser microscope
14. Standardization of the Feulgen reaction for absorption in DNA image cytometry: A review
15. Introduction to fluorescent probes: Properties, history and applications
16. Advances in automated 3D image analysis of cell populations imaged by confocal microscopy
17. Automated 3D montage synthesis from laser-scanning confocal images: Application to quantitative tissue-level cytological analysis
18. Blind deconvolution of three-dimensional laser scanning confocal imagery: Recent advances and testing
19. Three-dimensional analysis of contrast-filled microvessel diameters
20. Algorithms for automated cell counting in thick specimens from 3D confocal fluorescence microscopy data
21. Confocal laser scanning microscopy and 3D reconstruction of neuronal structures in human brain cortex
22. Adaptive 3D skeletonization of noisy images using NxNxN connectivity analysis windows and robust surface detection
23. Automated three-dimensional tracing of HRP stained neurons from a stack of brightfield optical slices
24. 3D image cytometry
25. Confocal 3-dimensional DNA image cytometry in thick tissue sections
26. Application and quantitative validation of computer-automated 3D counting of cell nuclei
27. Confocal laser scanning microscopy and three-dimensional reconstruction of cell clusters in serous fluids