Suitable experimental conditions are required to characterize interferon-α2b synthetic peptides

European Journal of Biochemistry

Volumn 267, Issue 18, 2000, Pages 5711-5716

  Blank, Viviana C ^a   Peña, Clara ^a   Roguin, Leonor P ^a,b  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b Instituto de Quimica y Fisicoquimica Biologicas Buenos Aires   (Argentina)

Author keywords

Antiproliferative activity; Binding properties; Interferon 2b; Interferon receptor interaction; Synthetic peptides

Indexed keywords

CYSTEINE; CYTOKINE RECEPTOR; DITHIOTHREITOL; EPITOPE; IODINE 125; MEMBRANE RECEPTOR; MERCAPTOETHANOL; RECOMBINANT ALPHA2B INTERFERON; SYNTHETIC PEPTIDE; UREA;

ARTICLE; BINDING KINETICS; CELL GROWTH; CELL PROLIFERATION; CONCENTRATION RESPONSE; DISSOCIATION; DRUG RECEPTOR BINDING; GEL FILTRATION; GROWTH INHIBITION; HUMAN; HUMAN CELL; ISOTOPE LABELING; MITOSIS INHIBITION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING;

BINDING SITES; CELL DIVISION; CELL LINE; CELL MEMBRANE; CHROMATOGRAPHY, GEL; CYSTEINE; DITHIOTHREITOL; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITOPES; HUMANS; INTERFERON ALFA-2B; MERCAPTOETHANOL; PEPTIDES; PROTEIN BINDING; UREA;

EID: 0033829689     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01618.x     Document Type: Article

References (25)

1. Interferons and their actions
2. Interferon receptors
3. The interferon system
4. Human leukocyte interferon alpha: Structure, pharmacology, and therapeutic applications
5. Structure-activity of type I interferons
6. Definition of receptor binding domains in interferon-α
7. The three-dimensional high resolution structure of human interferon α-2a determined by heteronuclear NMR spectroscopy in solution
8. Refined crystal structure of recombinant murine interferon-β at 2.15 Å Ari; resolution
9. Interferon receptor recognition peptides enhance the biological potency of interferon alphas
10. Influence of synthetic peptides, corresponding to fragments of the human α-2 interferon molecule, on the proliferation of lymphoblastoid cells in vitro. Growth inhibition and receptor binding
11. Anti-tumor activity of synthetic peptide fragments of the human interferon α-2
12. Identification of a linear epitope of interferon-α2b recognized by neutralizing monoclonal antibodies
13. Synthesis and properties of human growth hormone fragments
14. Methods for assessing immunologic and biological properties of iodinated peptide hormones
15. Iodination of proteins, glycoproteins and peptides using a solid-phase oxidizing agent, 1,3,4,6-Tetrachloro-3α,6α-diphenylglycoluril (Iodogen)
16. Measurement of cell numbers by means of endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cell surface antigens
17. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
18. How antigenic are antigenic peptides?
19. Genetic transfer of a functional human interferon α receptor into mouse cells: Cloning and expression of its cDNA
20. The human interferon α/β receptor: Characterization and molecular cloning
21. The type-I interferon receptor. The long and short of it
22. Cloning and characterization of soluble and transmembrane isoforms of a novel component of the murine type I interferon receptor, IFNAR 2
23. Contributions of cloned type I interferon receptor subunits to differential ligand binding
24. Mutant U5A cells are complemented by an interferon-alpha beta receptor subunit generated by alternative processing of a new member of a cytokine receptor gene cluster
25. Determination of residues involved in ligand binding and signal transmission in the human IFN-α receptor 2