Comparison of PCR detection of mecA with standard susceptibility testing methods to determine methicillin resistance in coagulase-negative staphylococci

Journal of Clinical Microbiology

Volumn 34, Issue 2, 1996, Pages 249-253

  York, Mary K ^a   Gibbs, Laurel ^a   Chehab, Farid ^a   Brooks, Geo F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COAGULASE; METICILLIN; OXACILLIN;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM CULTURE; COAGULASE NEGATIVE STAPHYLOCOCCUS; INTERMETHOD COMPARISON; LABORATORY TEST; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STAPHYLOCOCCUS INFECTION; STRAIN DIFFERENCE;

BACTERIA (MICROORGANISMS); METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCI; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS;

EID: 0030025467     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.2.249-253.1996     Document Type: Article

References (24)

1. Archer, G. L., and E. Pennell. 1990. Detection of methicillin resistance in staphylococci by using a DNA probe. Antimicrob. Agents Chemother. 34: 1720-1724
2. Brakstad, O. G., J. A. Maeland, and Y. Tveten. 1993 Multiplex polymerase chain reaction for detection of genes for Staphylococcus aureus thermonucleasc and methicillin resistance and correlation with oxacillin resistance APMIS 101:681-688.
3. Chambers, H. F. 1993. Detection of methicillin-resistant staphylococci. Infect. Dis Clin N. Am. 7:425-133.
4. Fass, R. J., V. L. Helsel, J. Barnishan, and L. W. Avers. 1986. In vitro susceptibilities of four species of coagulase-negative staphylococci. Antimicrob Agents Chemother. 30:545-552.
5. Geha, D. J., J. R. Uhl, C. A. Gustaferro, and D. H. Persing. 1994. Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory. J. Clin. Microbiol. 32:1768-1772.
6. Gerberding, J. L., C. Miick, H. H. Liu, and H. F. Chambers. 1991. Comparison of conventional susceptibility tests with direct detection of penicillin-binding protein 2a in borderline oxacillin-resistant strains of Staphylococcus aureus Antimicrob. Agents Chemother. 35:2574-2579.
7. Hedin, G., and S. Lofdahl. 1993 Detecting methicillin-resistant Staphylococcus epidermidis - disc diffusion, broth breakpoint or polymerase chain reaction? APMIS 101:311-318.
8. Huang, M. B., T. E. Gay, C. N. Baker, S. N. Banerjee, and F. C. Tenover. 1993. Two percent sodium chloride is required for susceptibility testing of staphylococci with oxacillin when using agar-based dilution methods J. Clin. Microbiol. 31:2683-2688.
9. Kellogg, J. A., and C. G. Garver. 1989 Improved detection of beta-lactamase activity in isolates of Staphylococcus saprophyticus with the use of a modified cefinase disk procedure. Am. J. Clin Pathol 91:319-322
10. Kloos, W. E., and T. L. Bannerman. 1994. Update on clinical significance of coagulase-negative staphylococci. Clin. Microbiol Rev 7:117-140.
11. McDonald, C. L., W. E. Maher, and R. J. Fass. 1995. Revised interpretation of oxacillin MICs for Staphylococcus epidermidis based on mecA detection Antimicrob Agents Chemother. 39:982-984.
12. Murakami, K., W. Minamide, K. Wada, E. Nakamura, H. Teraoka, and S. Watanabe. 1991 Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J. Clin. Microbiol 29:2240-2244.
13. National Committee for Clinical Laboratory Standards. 1993. Approved standard M2-A5 Performance standards for antimicrobial disk susceptibility tests. National Committee for Clinical Laboratory Standards, Villanova, Pa.
14. National Committee for Clinical Laboratory Standards. 1993. Approved standard M7-A3. Dilution antimicrobial susceptibility tests for bacteria that grow aerobically National Committee for Clinical Laboratory Standards, Villanova, Pa
15. Olsson-Liljequist, B., P. Larsson, S. Ringertz, and S. Lofdahl. 1993. Use of a DNA hybridization method to verify results of screening for methicillin resistance in staphylococci. Eur. J. Clin. Microbiol Infect Dis. 12:527-533
16. Pierre, J., R. Williamson, M. Bornet, and L. Gutmann. 1990. Presence of an additional penicillin-binding protein in methicillin-resistant Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus sunulans with a low affinity for methicillin, cephalothin, and cefamandole. Antimicrob. Agents Chemother 34:1691-1694.
17. Predari, S. C., M. Ligozzi, and R. Fontana. 1991. Genotypic identification of methicillin-resistant coagulase-negative staphylococci by polymerase chain reaction. Antimicrob Agents Chemother. 35:2568-2573.
18. Ryffel, C., F. H. Kayser, and B. Berger-Bachi. 1992. Correlation between regulation of mecA transcription and expression of methicillin resistance in staphylococci Antimicrob. Agents Chemother. 36:25-31.
19. Stratton, C. W., M. S. Gelfand, J. L. Gerberding, and H. F. Chambers. 1990. Characterization of mechanisms of resistance to beta-lactam antibiotics in methicillin-resistant strains of Staphylococcus saprophyticus Antimicrob Agents Chemother 34:1780-1782.
20. Suzuki, E., K. Hiramatsu, and T. Yokota. 1992. Survey of methicillin-resistant clinical strains of coagulase-negative staphylococci for mecA gene distribution. Antimicrob. Agents Chemother. 36:429-134.
21. Ubukata, K., S. Nakagami, A. Nitta, A. Yamane, S. Kawakami, M. Sugiura, and M. Konno. 1992. Rapid detection of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of polymerase chain reaction products. J. Clin Microbiol. 30:1728-1733.
22. Ubukata, K., R. Nonoguchi, M. D. Song, M. Matsuhashi, and M. Konno. 1990. Homology of mecA gene in methicillin-resistant Staphyhcoccus haemolyticus and Staphyhcoccus simulans to that of Staphyhcoccus aureus. Antimicrob Agents Chemother. 34:170-172
23. Unal, S., J. Hoskins, J. E. Flokowitsch, C. Y. Wu, D. A. Preston, and P. L. Skatrud. 1992. Detection of methicillin-resistant staphylococci by using the polymerase chain reaction. J. Clin. Microbiol. 30:1685-1691.
24. Woods, G. I., G. S. Hall, T. Rutherford, K. J. Pratt, and C. C. Knapp. 1986. Detection of methicillin-resistant Staphyhcoccus epidermidis. J. Clin Microbiol. 24:349-352