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19
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We have found that calicheamicin effects double-stranded cleavage of all the specific complexes. This exchange process is therefore not a general feature of calicheamicin binding and activity.
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Calicheamicin does not cleave mixed DNA sequences, as observed in numerous gel-electrophoresis experiments. We observed no binding of the native oligosaccharide to mixed sequences using capillary electrophoresis (see ref 17).
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32
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0343870563
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This complex was previously analyzed by Paloma et al. (ref 14) Because the exchange process made it impossible to assign the spectra at room temperature, these authors made their assignments at 45°C, a temperature at which conformational averaging simplified the spectra.
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33
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0343434814
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note
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36 also suggest that calicheamicin recognizes common conformational features first, and only secondarily discriminates between pyrimidine sequences based on specific contacts.
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