Protocol for genomic DNA preparation from fresh or frozen serum for PCR amplification

BioTechniques

Volumn 29, Issue 3, 2000, Pages 460-466

  Lin, Z ^a   Floros, J ^a  

^a PENN STATE COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; SURFACTANT PROTEIN A; SURFACTANT PROTEIN B;

ARTICLE; DNA PURIFICATION; DNA SEQUENCE; GENE AMPLIFICATION; HETEROZYGOSITY LOSS; HUMAN; HUMAN TISSUE; METHODOLOGY; POLYMERASE CHAIN REACTION; SERUM;

EID: 0033822961     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00293bm10     Document Type: Article

References (16)

1. K-ras mutations are found in DNA extracted from the plasma of patients with colorectal cancer
2. PCR-based genotyping can generate artifacts in LOH analyses
3. Microsatellite alterations in plasma DNA of small cell lung cancer patients
4. Methods for extracting and amplifying genomic DNA isolated from frozen serum
5. Dinucleotide repeats in the human surfactant protein B gene and respiratory distress syndrome
6. Genetic analysis of human DNA recovered from minute amounts of serum or plasma
7. Detection of cancer cells in peripheral blood of breast cancer patients using reverse transcription-polymerase chain reaction for epidermal growth factor receptor
8. An SP-B gene mutation responsible for SP-B deficiency in fatal congenital alveolar proteinosis: Evidence for mutation hotspot in exon 4
9. Genomic DNA extraction from small amounts of sera to be used for genotype analysis
10. Prenatal diagnosis of fetal RhD status by molecular analysis of maternal plasma
11. A method for using serum as a diagnosis test for congenital cytomegalovirus infection
12. Diagnosis of pancreatico-biliary malignancy: Detection of gene mutations in plasma and stool
13. An allelic variant of the 6A gene for human surfactant protein A
14. Direct PCR of small genomic DNA fragments from serum
15. Presence of tumor DNA in plasma of breast cancer patients: Clinicopathological correlations
16. Evaluation of different approaches for fetal DNA analysis from maternal plasma and nucleated blood cells