Development of a destabilized firefly luciferase enzyme for measurement of gene expression

BioTechniques

Volumn 29, Issue 3, 2000, Pages 590-601

  Leclerc, G M ^a   Boockfor, F R ^a   Faught, W J ^a   Frawley, L S ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LUCIFERASE;

ARTICLE; CANCER CELL CULTURE; DNA DETERMINATION; DNA RESPONSIVE ELEMENT; GENE EXPRESSION; GENE TECHNOLOGY; HUMAN; NONHUMAN; REPORTER GENE;

MAMMALIA;

EID: 0033822731     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00293rr02     Document Type: Article

References (32)

1. Multiple measurements of gene expression in single, living cells enable molecular analysis of endocrine cell heterogeneity
2. Dynamic monitoring and quantification of gene expression in single, living cells: A molecular basis for secretory cell heterogeneity
3. Luciferase from the East European firefly Luciola mingrelica: Cloning and nucleotide sequence of the cDNA, overexpression in Escherichia coli and purification of the enzyme
4. Firefly luciferase gene: Structure and expression in mammalian cells
5. Real time measurement of gene expression in living endocrine cells
6. Trypanosome ornithine decarboxylase is stable because it lacks sequences found in the carboxyl terminus of the mouse enzyme which target the latter for intracellular degradation
7. Prevention of rapid intracellular degradation of ODC by a carboxyl-terminal truncation
8. Rapid and regulated degradation of ornithine decarboxylase
9. Molecular genetics of polyamine synthesis in eukaryotic cells
10. The ubiquitin system for protein degradation
11. Nucleotide sequence of murine ornithine decarboxylase mRNA
12. Degradation of ornithine decarboxylase: Exposure of the C-terminal target by a polyamine-inducible inhibitory protein
13. Generation of destabilized green fluorescent protein as a transcription reporter
14. The C terminus of mouse ornithine decarboxylase confers rapid degradation on dihydrofolate reductase
15. Expression of the Renilla reniformis luciferase gene in mammalian cells
16. Isolation and expression of a cDNA encoding Renilla reniformis luciferase
17. Cloning and sequence analysis of cDNA for luciferase of a Japanese firefly, Luciola cruciata
18. RU486 exerts antiestrogenic activities through a novel progesterone receptor A form-mediated mechanism
19. Episodic gonadotropin-releasing hormone gene expression revealed by dynamic monitoring of luciferase reporter activity in single, living neurons
20. The mechanism by which cycloheximide and related glutarimide antibiotics inhibit peptide synthesis on reticulocyte ribosomes
21. PEST sequences are signals for rapid intracellular proteolysis
22. PEST sequences and regulation by proteolysis
23. Amino acid sequences common to rapidly degraded proteins: The PEST hypothesis
24. Real-time imaging of gene expression in single living cells
25. Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides
26. Modulation of firefly luciferase stability and impact on studies of gene regulation
27. Dynamics of stimulus-expression coupling as revealed by monitoring of prolactin promoter-driven reporter activity in individual, living mammotropes
28. Real-time monitoring of estrogen-regulated gene expression in single, living breast cancer cells: A new paradigm for the study of molecular dynamics
29. Phytoestrogens have agonistic and combinatorial effects on estrogen-responsive gene expression in MCF-7 human breast cancer cells
30. Bioluminescence
31. Complementary DNA coding click beetle luciferases can elicit bioluminescence of different colors