Isolation and characterization of a capsule-deficient mutant of Actinobacillus pleuropneumoniae serotype 1

Microbial Pathogenesis

Volumn 28, Issue 5, 2000, Pages 279-289

  Rioux, Stéphane ^a   Galarneau, Catherine ^a   Harel, Josée ^a   Kobisch, Marylène ^b   Frey, Joachim ^c   Gottschalk, Marcelo ^a   Jacques, Mario ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

^b Healthy Pigs Production and Experimentation Section   (France)

^c UNIVERSITY OF BERN   (Switzerland)

Author keywords

Actinobacillus pleuropneumoniae; Adherence; Capsule; Complement resistance; Pathogenicity; Transposon mutants

Indexed keywords

GENE PRODUCT; MONOCLONAL ANTIBODY;

ACTINOBACILLUS; ACTINOBACILLUS PLEUROPNEUMONIAE; ANIMAL TISSUE; ARTICLE; BACTERIAL PNEUMONIA; BACTERIUM ADHERENCE; BACTERIUM MUTANT; FLOW CYTOMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEROTYPE; SOUTHERN BLOTTING; SWINE DISEASE; TRACHEA; TRANSMISSION ELECTRON MICROSCOPY; TRANSPOSON;

ACTINOBACILLUS; ACTINOBACILLUS PLEUROPNEUMONIAE; ACTINOBACILLUS PLEUROPNEUMONIAE SEROVAR 1; ANIMALIA; BACTERIA (MICROORGANISMS); MYCOPLASMA; NEGIBACTERIA; SUIDAE; SUS SCROFA;

EID: 0033816954     PISSN: 08824010     EISSN: None     Source Type: Journal    
DOI: 10.1006/mpat.1999.0347     Document Type: Article

References (58)

1. Actinobacillus pleuropneumoniae
2. Serological characterization of Actinobacillus pleuropneumoniae strains and proposal of a new serotype: Serotype 12
3. Serological characterization of Actinobacillus pleuropneumoniae strains isolated from pigs in Quebec
4. Virulence in Actinobacillus pleuropneumoniae and RTX toxins
5. Role of lipopolysaccharides in adherence of Actinobacillus pleuropneumoniae to porcine tracheal rings
6. Affinity for porcine respiratory tract mucus is found in some isolates of Actinobacillus pleuropneumoniae
7. Role of lipo-oligosaccharides and lipopolysaccharides in bacterial adherence
8. High-molecular-mass lipopolysaccharides are involved in Actinobacillus pleuropneumoniae adherence to porcine respiratory tract cells
9. Effects of pig hemoglobin binding on some physical and biological properties of Actinobacillus pleuropneumoniae lipopolysaccharides
10. Lipopolysaccharides of Actinobacillus pleuropneumoniae bind pig hemoglobin
11. Virulence properties of Actinobacillus pleuropneumoniae
12. Virulence factors of the swine pathogen Actinobacillus pleuropneumoniae
13. Actinobacillus pleuropneumoniae infections in pigs: The role of virulence factors in pathogenesis and protection
14. Electron microscopic examination of capsular material from various serotypes of Actinobacillus pleuropneumoniae
15. Use of monoclonal antibodies to visualize capsular material of bacterial pathogens by conventional electron microscopy
16. Virulence properties and protective efficacy of the capsular polymer of Haemophilus (Actinobacillus) pleuropneumoniae serotype 5
17. Complement resistance in Actinobacillus (Haemophilus) pleuropneumoniae infection of swine
18. Resistance of Actinobacillus pleuropneumoniae to bactericidal antibody and complement is mediated by capsular polysaccharide and blocking antibody specific for lipopolysaccharide
19. Cloning and mutagenesis of a serotype-specific DNA region involved in encapsulation and virulence of Actinobacillus pleuropneumoniae serotype 5a: Concomitant expression of serotype 5a and 1 capsular polysaccharides in recombinant A. pleuropneumoniae serotype 1
20. Structural characteristics of the antigenic capsular polysaccharides and lipopolysaccharides involved in the serological classification of Actinobacillus (Haemophilus) pleuropneumoniae strains
21. Identification and characterization of a DNA region involved in the export of capsular polysaccharide of Actinobacillus pleuropneumoniae serotype 5a
22. The bex locus in encapsulated Haemophilus influenzae: A chromosomal region involved in capsule polysaccharide export
23. Evidence for a common molecular origin of the capsule gene loci in gram-negative bacteria expressing group II capsular polysaccharides
24. Cloning, sequencing, expression, and complementation analysis of the Escherichia coli K1 kps region 1 gene, kpsE, and identification of an upstream open reading frame encoding a protein with homology to GutQ
25. Identification of two genes, kpsM and kpsT, in region 3 of the polysialic acid gene cluster of Escherichia coli K1
26. Molecular analysis of region 1 of the Escherichia coli K5 antigen gene cluster: A region encoding proteins involved in cell surface expression of capsular polysaccharide
27. Characterization and localization of the KpsE protein of Escherichia coli K5, which is involved in polysaccharide export
28. Molecular analysis of the Escherichia coi K5 kps locus: Identification and characterization of an inner-membrane capsular polysaccharide transport system
29. Safety, stability, and efficacy of noncapsulated mutants of Actinobacillus pleuropneumoniae for use in live vaccines
30. Serotype specificity and immunogenicity of the capsular polymer of Haemophilus pleuropneumoniae serotype 5
31. Characterization of an attenuated strain of Actinobacillus pleuropneumoniae, serotype 1
32. Isolation and characterization of mini-Tn10 lipopolysaccharide mutants of Actinobacillus pleuropneumoniae serotype 1
33. Production and characterization of monoclonal antibodies against Actinobacillus pleuropneumoniae serotype 1
34. The respiratory system
35. Adherence of Actinobacillus pleuropneumoniae to porcine tracheal epithelial cells and frozen lung sections
36. Proteins found within porcine respiratory tract secretions bind lipopolysaccharides of Actinobacillus pleuropneumoniae
37. In vivo association of Actinobacillus pleuropneumoniae serotype 2 with the respiratory epithelium of pigs
38. Influence of Actinobacillus pleuropneumoniae and its metabolites on porcine alveolar epithelial cells
39. Loss of capsule expression by Haemophilus influenzae type b results in enhanced adherence to and invasion of human cells
40. Interactions of Haemophilus influenzae with cultured human endothelial cells
41. Virulence of capsulated and noncapsulated isolates of Pasteurella multocida and their adherence to porcine respiratory tract cells and mucus
42. Consequence of reduction of Klebsiella pneumoniae capsule expression on interactions of this bacterium with epithelial cells
43. Effect of the (α2-8)-linked polysialic acid capsule on adherence of Neisseria meningitidis to human mucosal cells
44. Expression of the Opc protein correlates with invasion of epithelial and endothelial cells by Neisseria meningitidis
45. Meningococcal Opa and Opc proteins: Their role in colonization and invasion of human epithelial and endothelial cells
46. Capsule reduce adherence of enterotoxigenic Escherichia coli to isolated intestinal epithelial cells of pigs
47. Comparison of loss of serum resistance by defined lipopolysaccharide mutants and an non-capsular mutant of uropathogenic Escherichia coli O75:K5
48. Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5- and Tn10-derived minitransposons
49. Transposon mutagenesis in Actinobacillus pleuropneumoniae with a Tn10 derivative
50. Isolation and characterization of LPS mutants of Actinobacillus pleuropneumoniae serotype 1
51. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels
52. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
53. Identification of concanavalin A-binding proteins after sodium dodecyl sulfate-gel electrophoresis and protein blotting
54. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate
55. A comprehensive set of sequence analysis programs for the VAX
56. Characterization of a polysaccharide capsular antigen of septicemic Escherichia coli O115:K'V165':F165 and evaluation of its role inpathogenicity
57. Reproducible pneumonia in gnotobiotic piglets induced with broth cultures of Mycoplasma hyopneumoniae and the effect of animal passage on virulence