A point mutation in the pore region alters gating, Ca2+ blockage, and permeation of olfactory cyclic nucleotide-gated channels

Journal of General Physiology

Volumn 116, Issue 3, 2000, Pages 311-325

  Gavazzo, Paola ^a   Picco, Cristiana ^a   Eismann, Elisabeth ^b   Kaupp, U Benjamin ^b   Menini, Anna ^a,c  

^a CNR   (Italy)

^b FORSCHUNGSZENTRUM JÜLICH   (Germany)

^c SISSA   (Italy)

Author keywords

Cyclic adenosine monophosphate; Cyclic guanosine monophosphate; Olfactory sensory neurons; Site directed mutagenesis

Indexed keywords

CALCIUM CHANNEL; CALCIUM ION; CYCLIC AMP; CYCLIC GMP; ION CHANNEL; MAGNESIUM ION; SODIUM CHANNEL; SODIUM ION;

ANIMAL CELL; ARTICLE; CATTLE; NONHUMAN; OLFACTORY NERVOUS SYSTEM; POINT MUTATION; RETINA ROD; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS;

ANIMALS; CALCIUM; CATTLE; CYCLIC AMP; CYCLIC GMP; FEMALE; ION CHANNEL GATING; ION CHANNELS; MEMBRANE POTENTIALS; MODELS, BIOLOGICAL; MUTAGENESIS, SITE-DIRECTED; OLFACTORY RECEPTOR NEURONS; OOCYTES; PERMEABILITY; POINT MUTATION; RECEPTORS, ODORANT; RECOMBINANT PROTEINS; XENOPUS LAEVIS;

EID: 0033815963     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.116.3.311     Document Type: Article

References (54)

1. Control of ligand specificity in cyclic nucleotide-gated channels from rod photoreceptors and olfactory epithelium
2. Cyclic nucleotide-gated channels. Pore topology studied through the accessibility of reporter cysteines
3. The native rat olfactory cyclic nucleotide-gated channel is composed of three distinct subunits
4. Heteromeric olfactory cyclic nucleotide-gated channels: A subunit that confers increased sensitivity to cAMP
5. Specific labeling and permanent activation of the retinal rod cGMP-activated channel by the photoaffinity analog 8-p-azidophenacylthio-cGMP
6. Movement of gating machinery during the activation of rod cyclic nucleotide-gated channels
7. Single-channel properties of ionic channels gated by cyclic nucleotides
8. Blockage and permeation of divalent cations through the cyclic GMP-activated channel from tiger salamander retinal rods
9. Primary structure and functional expression of a cyclic nucleotide-activated channel from olfactory neurons
10. 2+ permeation in cyclic nucleotide-gated channels
11. 2+ blockage, and ionic selectivity
12. Computer programs for calculating total from specified free or free from specified total ionic concentrations in aqueous solutions containing multiple metals and ligands
13. Cyclic nucleotide-gated ion channels: An extended family with diverse functions
14. Tetracaine reports a conformational change in the pore of cyclic nucleotide-gated channels
15. Profoundly different calcium permeation and blockage determine the specific function of distinct cyclic nucleotide-gated channels
16. Mechanisms of modulation by internal protons of cyclic nucleotide-gated channels cloned from sensory receptor cells
17. Localization of regions affecting an allosteric transition in cyclic nucleotide-activated channels
18. Direct interaction between amino- and carboxyl-terminal domains of cyclic nucleotide-gated channels
19. Molecular cloning and single channel properties of the cyclic nucleotide-gated channel from catfish olfactory neurons
20. Molecular mechanism of cyclic-nucleotide-gated channel activation
21. Divalent cation selectivity is a function of gating in native and recombinant cyclic nucleotide-gated ion channels from retinal photoreceptors
22. A general and rapid mutagenesis method using polymerase chain reaction
23. Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel
24. Family of cyclic nucleotide gated ion channels
25. Block by external calcium and magnesium of the cyclic nucleotide-activated current in olfactory cilia
26. Both external and internal calcium reduce the sensitivity of the olfactory cyclic nucleotide-gated channel to cAMP
27. A second subunit of the olfactory cyclic nucleotide-gated channel confers high sensitivity to cAMP
28. Calcium-calmodulin modulation of the olfactory cyclic nucleotide-gated cation channel
29. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter
30. The effect of pH on the cyclic GMP-activated conductance in retinal rods
31. Cyclic nucleotide-gated channels in visual and olfactory transduction
32. Calcium signalling and regulation in olfactory neurons
33. A cyclic nucleotide-gated conductace in olfactory receptor cilia
34. C-linker of cyclic nucleotide-gated channels controls coupling of ligand binding to channel gating
35. Divalent cation selectivity in a cyclic nucleotide-gated ion channel
36. Response properties of cones from the retina of the tiger salamander
37. Permeability and interaction of Ca with cGMP-gated ion channels differ in retinal rod and cone photoreceptors
38. Identification of an external divalent cation-binding site in the pore of a cGMP-activated channel
39. Two identical noninteracting sites in an ion channel revealed by proton transfer
40. DNA sequencing with chain-terminating inhibitors
41. An isoform of the rod photoreceptor cyclic nucleotide-gated channel beta subunit expressed in olfactory neurons
42. Three residues predicted by molecular modeling to interact with the purine moiety alter ligand binding and channel gating in cyclic nucleotide-gated channels
43. 2+-binding site in the pore of cyclic nucleotide-gated channels: S5/S6 segments control affinity of intrapore glutamates
44. Exposure of residues in cyclic nucleotide-gated channel pore: P region structure and function in gating
45. Mechanism of allosteric modulation of rod cyclic nucleotide-gated channels
46. Sequence of events underlying the allosteric transition of rod cyclic nucleotide-gated channels
47. Allosteric activation and tuning of ligand efficacy in cyclic nucleotide-gated channels
48. Molecular mechanism for ligand discrimination of cyclic nucleotide-gated channels
49. Interdomain interactions underlying activation of cyclic nucleotide gated channels
50. Structure and function of cyclic nucleotide-gated channels
51. Cyclic nucleotide-gated channels
52. Cation interactions within the cyclic GMP-activated channel of retinal rods from the tiger salamander
53. Hindered diffusion in excised membrane patches from retinal rods outer segments
54. Three amino acids in the C-linker are major determinants of gating in cyclic nucleotide-gated channel