Insights into nucleotide binding in protein kinase A using fluorescent adenosine derivatives

Protein Science

Volumn 9, Issue 9, 2000, Pages 1818-1827

  Ni, D Q ^a   Shaffer, J ^a   Adams, J A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Fluorescent nucleotides; Protein kinase A; Stopped flow spectroscopy; Transient state kinetics

Indexed keywords

ADENOSINE DERIVATIVE; ANTHRANILIC ACID METHYL ESTER; CYCLIC AMP DEPENDENT PROTEIN KINASE; NUCLEOTIDE;

ARTICLE; BINDING AFFINITY; CATALYSIS; CONFORMATIONAL TRANSITION; DERIVATIZATION; DISSOCIATION CONSTANT; ENZYME ACTIVE SITE; ENZYME BINDING; FLUORESCENCE; ISOMER; KINETICS; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN CONFORMATION; REVERSED PHASE LIQUID CHROMATOGRAPHY; SPECTROSCOPY;

EID: 0033815252     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.9.9.1818     Document Type: Article

References (43)

1. Energetic limits of phosphotransfer in the catalytic subunit of cAMP-dependent protein kinase as measured by viscosity experiments
2. Divalent metal ions influence catalysis and activesite accessibility in the cAMP-dependent protein kinase
3. Magnetic resonance and kinetic studies of the manganese(II) ion and substrate complexes of the catalytic subunit of adenosine 3',5'-monophosphate dependent protein kinase from bovine heart
4. Cyclic AMP-dependent ATPase activity of bovine heart protein kinase
5. Adenosine cyclic 3'-5'-monophosphate dependent kinase: A new fluorescence displacement titration technique for characterizing the nucleotide binding site on catalytic subunit
6. ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeflciency
7. Interaction of mant-adenosine nucleotides and magnesium with kinesin
8. Mapping substrate-induced conformational changes in cAMP-dependent protein kinase by protein footprinting
9. Molecular basis of insulin resistance
10. The development and therapeutic potential of protein kinase inhibitors
11. Adenosine cyclic 3',5'-monophosphate dependent protein kinase: Kinetic mechanism for the bovine skeletal muscle catalytic subunit
12. Human severe combined immunodeficiency due to a defect in ZAP-70, a T cell kinase
13. Transient kinetic approaches to enzyme mechanisms
14. Kinetic characterization of myosin head fragments with long-lived myosin. ATP states
15. Pre-steady-state kinetic analysis of cAMP-dependent protein kinase using rapid quench flow techniques
16. Adenosine cyclic 3'-5'-monophosphate dependent protein kinase: Interaction of the catalytic subunit and holoenzyme with lin-benzoadenine nucleotides
17. New ribose-modified fluorescent analogs of adenine and guanine nucleotides available as substrates for various enzymes
18. Mapping the ATP binding site in the catalytic subunit of cAMP-dependent protein kinase; spatial relationship with the ATP binding site of the undissociated enzyme
19. Transiem-state kinetic analysis of enzyme reaction pathways
20. Crystal structure of the catalytic subunit of cAMP-dependent protein kinase
21. Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase
22. Isotope partitioning in the adenosine 3'-5'-monophosphate dependent protein kinase reaction indicates a steady-state random kinetic mechanism
23. Identification of a partially rate-determining step in the catalytic mechanism of cAMP-dependent protein kinase: A transient kinetic study using stopped-flow fluorescence spectroscopy
24. Biochemistry of the Src protein-tyrosine kinase: Regulation by SH2 and SH3 domains
25. Kinetic mechanism of a monomeric kinesin construct
26. cAMP-dependent protein kinase: Crystallographic insights into substrate recognition and phosphotransfer
27. Heat stable protein derived peptide substrate analogs: Phosphorylation by cAMP-dependent and cGMP-dependent protein kinase
28. Probing the nucleotide binding sites of axonemal dynein with the fluorescent nucleotide analogue 2'(3')-O-(-N-methylanthraniloyl)-adenosine 5'-triphosphate
29. Pathway of ATP hydrolysis by monomeric and dimeric kinesin
30. A binary complex of the catalytic subunit of cAMP-dependent protein kinase and adenosine further defines conformational flexibility
31. Solution structure of the cAMP-dependent protein kinase catalytic subunit and its contraction upon binding the protein kinase inhibitor peptide
32. Signaling through scaffold, anchoring, and adaptor proteins
33. Mutation of unique region of Bruton's tyrosine kinase in immunodeficient XID mice
34. An ATP-linked structural change in protein kinase A precedes phosphoryl transfer under physiological magnesium concentrations
35. Detection of conformational changes along the kinetic pathway of protein kinase A using a catalytic trapping technique
36. Colocalization of X-linked agammaglobulinemia and X-linked immunodeficiency genes
37. Mechanism of 2-chloro-(ε-amino-Lys75)-[6-[4-(N,N-diethylamino)phenyl]-1,3,5-triazin-4-yl] calmodulin interactions with smooth muscle myosin light chain kinase and derived peptides
38. Kinetics of the interaction of 2'(3')-O-(N-methylanthraniloyl)-ATP with myosin subfragment 1 and actomyosin subfragment 1: Characterization of two acto-S1-ADP complexes
39. Prokaryotic expression of the catalytic subunit of cAMP-dependent protein kinase
40. Crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MgATP and peptide inhibitor
41. Crystal structures of the myristylated catalytic subunit of cAMP-dependent protein kinase reveal open and closed conformations
42. Participation of ADP dissociation in the rate-determinig step in cAMP-dependent protein kinase