Overcoming the restriction barrier to plasmid transformation of Helicobacter pylori

Molecular Microbiology

Volumn 37, Issue 5, 2000, Pages 1066-1074

  Donahue, John P ^a   Israel, Dawn A ^a,b   Peek Jr , Richard M ^a,b   Blaser, Martin J ^a,b,c   Miller, Geraldine G ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

^c NEW YORK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA METHYLATION; GENE TRANSFER; HELICOBACTER PYLORI; NONHUMAN; PLASMID; PRIORITY JOURNAL;

BACTERIAL PROTEINS; CELL-FREE SYSTEM; DNA METHYLATION; DNA, BACTERIAL; HELICOBACTER PYLORI; PLASMIDS; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); SPECIES SPECIFICITY; TRANSFORMATION, BACTERIAL;

EID: 0033812766     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.02036.x     Document Type: Article

References (37)

1. PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori
2. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
3. Restriction-modification system differences in Helicobacter pylori are a barrier to interstrain plasmid transfer
4. The clinical and pathological importance of heterogeneity in vacA, encoding the vacuolating cytotoxin of Helicobacter pylori
5. Identification of virulence genes of Helicobacter pylori by random insertion mutagenesis
6. A novel approach for the construction of a Campylobacter mutant library
7. cag, A pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors
8. FACS-optimized mutants of the green fluorescent protein (GFP)
9. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer
10. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains
11. Analysis of iceA1 transcription in Helicobacter pylori
12. Prevalence of Helicobacter pylori infection and histologic gastritis in asymptomatic persons
13. Multiple restriction-modification systems in Neisseria gonorrhoeae
14. Role of the vacuolating toxin from Helicobacter pylori in the pathogenesis of duodenal and gastric ulcer
15. Restriction and modification of a self-complementary octanucleotide containing the EcoRI substrate
16. Aflagellated mutants of Helicobacter pylori generated by genetic transformation of naturally competent strains using transposon shuttle mutagenesis
17. Genetic organization of a small cryptic plasmid of Helicobacter pylori
18. A stable shuttle vector system for efficient genetic complementation of Helicobacter pylori strains by transformation and conjugation
19. Rapid and efficient cosmid cloning
20. Characterization of a plasmid from Helicobacter pylori encoding a replication protein common to plasmids in gram-positive bacteria
21. Gene disruption and replacement as a feasible approach for mutagenesis of Campylobacter jejuni
22. Construction of a Helicobacter pylori-Escherichia coli shuttle vector for gene transfer in Helicobacter pylori
23. A standardized mouse model of Helicobacter pylori infection: Introducing the Sydney strain
24. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration
25. Helicobacter pylori infection and gastric carcinoma in a population of Japanese-Americans in Hawaii
26. Helicobacter pylori infection and gastric lymphoma
27. Adherence to gastric epithelial cells induces expression of a Helicobacter pylori gene, iceA, that is associated with clinical outcome
28. Helicobacter pylori and peptic ulcer disease
29. Type II restriction endonucleases
30. Restriction of plasmid DNA during transformation but not conjugation in Neisseria gonorrhoeae
31. The complete genome sequence of the gastric pathogen Helicobacter pylori
32. Genetic transformation in Helicobacter pylori
33. Cloning and expression of a high molecular weight major antigen of Helicobacter pylori: Evidence of linkage to cytotoxin production
34. Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker
35. Genetic manipulation of Campylobacter - Evaluation of natural transformation and electro-transformation
36. The Helicobacter pylori genome is modified at CATG by the product of hpyIM