Biochemical characterization and localization of the dual specificity kinase CLK1

Journal of Cell Science

Volumn 113, Issue 18, 2000, Pages 3241-3253

  Menegay, H J ^a   Myers, M P ^a   Moeslein, F M ^a   Landreth, G E ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cytoplasmic; Neuronal; Phosphatase

Indexed keywords

AMINO ACID; CYCLOHEXIMIDE; ISOPROTEIN; MESSENGER RNA; PHOSPHATASE; PHOSPHOTRANSFERASE; PROTEIN KINASE; REGULATOR PROTEIN; SERINE; THREONINE; TYROSINE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; CATALYSIS; CELL NUCLEUS; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME LOCALIZATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; MOUSE; NERVE CELL; NONHUMAN; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; RAT; REGULATORY MECHANISM; RNA SPLICING;

3T3 CELLS; AMINO ACID SEQUENCE; ANIMAL; BINDING SITES; BLOTTING, WESTERN; BRAIN; CONSENSUS SEQUENCE; MICE; MOLECULAR SEQUENCE DATA; MYELIN BASIC PROTEINS; PC12 CELLS; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; PROTEIN-TYROSINE KINASE; RABBITS; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA SPLICING; RNA, MESSENGER; STAINING AND LABELING; SUBSTRATE SPECIFICITY; SUPPORT, U.S. GOV'T, P.H.S.; TRANSFECTION;

EID: 0033785992     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (51)

1. A mammalian protein kinase with potential for serine/threonine and tyrosine phosphorylation is related to cell cycle regulators
2. AFC1, a LAMMER kinase from Arabidopsis thaliana, activates STE12-dependent processes in yeast
3. Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates
4. A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm
5. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
6. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors
7. The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution
8. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product
9. Mice lacking nerve growth factor display perinatal loss of sensory and sympathetic neurons yet develop basal forebrain cholinergic neurons
10. Phosphorylation sites in the autoinhibitory domain participate in p70(s6k) activation loop phosphorylation
11. Protein phosphorylation plays an essential role in the regulation of alternative splicing and sex determination in Drosophila
12. Alternative splicing of STY, a nuclear dual specificity kinase
13. In vivo regulation of alternative pre-mRNA splicing by the Clk1 protein kinase
14. The Clk2 and Clk3 dual-specificity protein kinases regulate the intranuclear distribution of SR proteins and influence pre-mRNA splicing
15. Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase
16. Effect of protein synthesis inhibitors on growth factor activation of c-fos, c-myc, and actin gene transcription
17. Characterization by cDNA cloning of two new human protein kinases. Evidence by sequence comparison of a new family of mammalian protein kinases
18. Protein kinases 6. The eukaryotic protein kinase superfamily: Kinase (catalytic) domain structure and classification
19. Protein kinase catalytic domain sequence database: Identification of conserved features of primary structure and classification of family members
20. The protein kinase family: Conserved features and deduced phylogeny of the catalytic domains
21. STY, a tyrosine-phosphorylating enzyme with sequence homology to serine/threonine kinases
22. Dynamic organization of pre-mRNA splicing factors
23. Animal Tissue Techniques
24. In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism
25. Molecular cloning of a novel human cdc2/CDC28-like protein kinase
26. Activity and autophosphorylation of LAMMER protein kinases
27. Raf-1: A kinase currently without a cause but not lacking in effects
28. Dual-specificity protein kinases: Will any hydroxyl do?
29. Cyanogen bromide cleavage and proteolytic peptide mapping of proteins immobilized to membranes
30. RNA processing. Splicing in space
31. Removal of a 67-base-pair sequence in the noncoding region of protooncogene fos converts it to a transforming gene
32. The dual specificity protein kinase CLK3 is abundantly expressed in mature mouse spermatozoa
33. The CLK family kinases, CLK1 and CLK2, phosphorylate and activate the tyrosine phosphatase, PTP-1B
34. The dual-specificity CLK kinase induces neuronal differentiation of PC12 cells
35. Characterization and comparison of four serine- and arginine-rich (SR) protein kinases
36. The cellular localization of the murine serine/arginine-rich protein kinase CLK2 is regulated by serine 141 autophosphorylation
37. Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase)
38. Immobilization of enzymes to agar, agarose, and Sephadex supports
39. The protein kinase Clk/Sty directly modulates SR protein activity: Both hyper- and hypophosphorylation inhibit splicing
40. Inhibition of protein synthesis stimulates the transcription of human beta-interferon genes in Chinese hamster ovary cells
41. Negative regulation of mitosis by wee1+, a gene encoding a protein kinase homolog
42. Nerve growth factor stimulates the tyrosine phosphorylation of MAP2 kinase in PC12 cells
43. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation
44. Severe sensory and sympathetic neuropathies in mice carrying a disrupted Trk/NGF receptor gene
45. Regulation of the Src protein tyrosine kinase
46. How do protein kinases discriminate between serine/threonine and tyrosine? Structural insights from the insulin receptor protein-tyrosine kinase
47. Signal transduction by receptors with tyrosine kinase activity
48. Rho family members: Activators of MAP kinase cascades
49. SRPK2: A differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells
50. The Doa locus encodes a member of a new protein kinase family and is essential for eye and embryonic development in Drosophila melanogaster