Generation of new hydrogen-recycling Rhizobiaceae strains by introduction of a novel hup minitransposon

Applied and Environmental Microbiology

Volumn 66, Issue 10, 2000, Pages 4292-4299

  Bascones, E ^a   Imperial, J ^a   Ruiz Argueso, T ^a   Palacios, J M ^a  

^a UNIVERSIDAD POLITÉCNICA DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISERUM; HYDROGEN; HYDROGENASE; NITROGENASE;

ARTICLE; BRADYRHIZOBIUM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBUNIT; GENE DELETION; GENETIC TRANSCRIPTION; IMMUNOBLOTTING; LEGUME; NITROGEN FIXATION; NONHUMAN; RECYCLING; RHIZOBIACEAE; RHIZOBIUM ETLI; RHIZOBIUM LEGUMINOSARUM; SINORHIZOBIUM MELILOTI; STRAIN DIFFERENCE; TRANSPOSON;

BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT; BRADYRHIZOBIUM; CLONING, MOLECULAR; DNA TRANSPOSABLE ELEMENTS; HYDROGEN; MOLECULAR SEQUENCE DATA; NITROGEN FIXATION; NITROGENASE; PLANTS; PLASMIDS; RHIZOBIACEAE; RHIZOBIUM LEGUMINOSARUM; SUPPORT, NON-U.S. GOV'T; SYMBIOSIS; TRANSCRIPTION, GENETIC;

BRADYRHIZOBIUM JAPONICUM; MESORHIZOBIUM LOTI; RHIZOBIUM LEGUMINOSARUM;

EID: 0033779176     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.10.4292-4299.2000     Document Type: Article

References (58)

1. Integration of hup genes into the genome of chickpea-Rhizobium through site-specific recombination
2. Alfalfa yield response to inoculation with recombinant strains of Rhizobium meliloti with an extra copy of dctABD and/or modified nifA expression
3. Recombinant nodulation plasmids in Rhizobium leguminosarum
4. 2 fixing simbionts
5. Hydrogenase genes from Rhizobium leguminosarum bv. viciae are controlled by the nitrogen fixation regulatory protein NifA
6. - rhizobia
7. Identification of a gene for a chemoreceptor of the methyl-accepting type in the symbiotic plasmid of Rhizobium leguminosarum bv. viciae UPM791
8. Nickel availability to pea (Pisum sarivum L.) plants limits hydrogenase activity of Rhizobium leguminosarum bv. viciae bacteroids by affecting the processing of the hydrogenase structural subunits
9. A novel autoregulation mechanism of fnrN expression in Rhizobium leguminosarum bv viciae
10. Clustering of nitrogen fixation (nif) genes in Rhizobium meliloti
11. Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5- and Tn10-derived minitransposons
12. Influence of the Bradyrhizobium hydrogenase on the growth of Glycine and Vigna species
13. The quinonereactive Ni/Fe-hydrogenase of Wolinella succinogenes
14. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers
15. Physiology, biochemistry, and genetics of the uptake hydrogenase in Rhizobia
16. The importance of hydrogen recycling in nitrogen fixation by legumes
17. Genetic regulation of nitrogen fixation in rhizobia
18. FnrN controls symbiotic nitrogen fixation and hydrogenase activities in Rhizobium leguminosarum bv viciae UPM791
19. The hypBFCDE operon from Rhizobium leguminosarum bv. viciae is expressed from an Fnr-type promoter that escapes mutagenesis of the fnrN gene
20. Transposon vectors containing non-antibiotic selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria
21. Nucleotide sequence of the hydrogenase structural genes from Rhizobium leguminosarum
22. Nucleotide sequence and characterization of four additional genes of the hydrogenase structural operon from Rhizobium leguminosarum bv. viciae
23. HupK, a hydrogenase-ancillary protein from Rhizobium leguminosarum, shares structural motifs with the large subunit of NiFe hydrogenases and could be a scaffolding protein for hydrogenase metal cofactor assembly
24. A transposable partitioning locus used to stabilize plasmid-borne hydrogen oxidation and trifolitoxin production genes in a Sinorhizobium strain
25. Expression of chromosome-integrated hydrogen-uptake genes in Cicer-Rhizobium
26. Symbiotic expression of cosmid-borne Bradyrhizobium japonicum hydrogenase genes
27. Cloning and characterization of hydrogen uptake genes from Rhizobium leguminosarum
28. Genetic organization of the hydrogen uptake (hup) cluster from Rhizobium leguminosarum
29. Environmental control of nitrogen fixation: Limitation to and flexibility of the legume-Rhizobium system
30. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: Application to open reading frame characterization
31. Biochemistry, regulation and genetics of hydrogen oxidation in Rhizobium
32. Towards more productive, efficient and competitive nitrogen-fixing symbiotic bacteria
33. Relative energy efficiency of nitrogen fixation by nodules of chickpea (Cicer arietinum L.) produced by different strains of Rhizobium
34. Evaluation and improvement of the energy efficiency of nitrogen fixation in Lotus corniculatus nodules induced by Rhizobium loti strains indigenous to Uruguay
35. Rhizobium phaseoli symbiotic mutants with transposon Tn5 insertions
36. Differential expression of hydrogen uptake (hup genes) in vegetative and symbiotic cells of Rhizobium leguminosarum
37. Nitrogen uptake transport and utilization
38. 2
39. 10-formyl tetrahydrofolate-dependent enzyme family and required for nickel-dependent hydrogenase processing and activity
40. 2-uptake gene cluster from Rhizobium leguminosarum bv viciae containing a rubredoxin-like gene and four additional open reading frames
41. Purification of Rhizobium leguminosarum HypB: A nickel-binding protein required for hydrogenase synthesis
42. Molecular analysis of a microaerobically induced operon required for hydrogenase synthesis in Rhizobium leguminosarum biovar viciae
43. Characterization of a protease from Escherichia coli involved in hydrogenase maturation
44. 2-uptake hydrogenase in the free-living state
45. Hydrogen production and uptake by pea nodules as affected by strains of Rhizobium leguminosarum
46. Uptake hydrogenases in root nodule bacteria
47. Multicopy plasmids carrying the Klebsiella pneumoniae nifA gene enhance Rhizobium meliloti nodulation competitiveness on alfalfa
48. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: Selection of defined deletions in the chromosome of Corynebacterium glutamicum
49. Hydrogen evolution: A major factor affecting the efficiency of nitrogen fixation in nodulated symbionts
50. Vector plasmids for in-vivo and in-vitro manipulations of Gram-negative bacteria
51. A nitrogen pressure of 50 atmospheres does not prevent evolution of hydrogen by nitrogenase
52. Measurement of protein using bicinchoninic acid
53. Identification of a potential transcriptional regulator of hydrogenase activity in free-living Bradyrhizobium japonicum strains
54. Imparting hydrogenrecycling capability to Cicer-rhizobial strains by plasmid pIJ1008 transfer
55. Transfer and expression of Bradyrhizobium japonicum uptake hydrogenase genes in Cicer-rhizobia
56. β-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other Gram-negative bacteria
57. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors