Polyvinylamine-streptavidin complexes labeled with a europium chelator: A universal detection reagent for solid-phase time resolved fluorometric applications

Clinical Biochemistry

Volumn 33, Issue 5, 2000, Pages 345-350

  Scorilas, Andreas ^a   Diamandis, Eleftherios P ^b  

^a UNIVERSITY OF TORONTO   (Canada)

^b MOUNT SINAI HOSPITAL   (Canada)

Author keywords

Afetoprotein; BCPDA; Biotin; NHS LC LC biotin; PSA; PVA; Streptavidin; Time resolved fluoroimmunoassay

Indexed keywords

CHELATING AGENT; EUROPIUM; POLYVINYL DERIVATIVE; POLYVINYLAMINE; STREPTAVIDIN; UNCLASSIFIED DRUG;

ARTICLE; CHELATION; COMPLEX FORMATION; FLUOROIMMUNOASSAY; ISOTOPE LABELING; PRIORITY JOURNAL; SYNTHESIS;

ANIMAL; BIOTIN; CALIBRATION; CHELATING AGENTS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DOSE-RESPONSE RELATIONSHIP, DRUG; EUROPIUM; FLUORESCENT DYES; FLUOROIMMUNOASSAY; FLUOROMETRY; INDICATORS AND REAGENTS; MICE; PHENANTHROLINES; POLYVINYLS; SENSITIVITY AND SPECIFICITY; STREPTAVIDIN; SUPPORT, NON-U.S. GOV'T; TIME FACTORS;

EID: 0033778895     PISSN: 00099120     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-9120(00)00082-5     Document Type: Article

References (27)

1. Fluoroimmunoassays and immunofluorometric assays
2. Europium chelate labels in time-resolved fluorescence immunoassays and DNA hybridization assays
3. Immunoassays with time-resolved fluorescence spectroscopy: Principles and applications
4. Time-resolved fluorometry in nucleic acid hybridization and Western blotting techniques
5. Lanthanides as probes for time-resolved fluorometric immunoassays
6. A new tetradentate beta-diketonate-europium chelate that can be covalently bound to proteins for time-resolved fluoroimmunoassay
7. Development of instrumentation and fluorochromes for automated multiparameter analysis of cells
8. Enzymatically amplified time-resolved fluorescence immunoassay with terbium chelates
9. A new europium chelate for protein labeling and time-resolved fluorometric applications
10. Streptavidin-based macromolecular complex labelled with a europium chelator suitable for time-resolved fluorescence immunoassay applications
11. Ultrasensitive prostate-specific antigen assays and their clinical application
12. Ultrasensitive detection of prostate specific antigen by a time-resolved immunofluorometric assay and the Immulite® immunochemiluminescent third generation assay: Potential applications in prostate and breast cancers
13. Is prostate-specific antigen present in female serum?
14. Time-resolved detection of lanthanide luminescence for ultrasensitive bioanalytical assays
15. Terbium chelates for fluorescence immunoassays
16. Bioluminescence and chemiluminescence literature. The 1996 literature: Part 1
17. Prospects for chemiluminescent and bioluminescent immunoassay and nucleic acid assays in food testing and the pharmaceutical industry
18. Nucleic acid detection technologies - Labels, strategies, and formats
19. Multianalyte immunoassay based on spatially distinct fluorescent areas quantified by laser-excited solid-phase time-resolved fluorometry
20. Ligand assays: From electrophoresis to miniaturized microarrays
21. Microarrays: Their origins and applications
22. Miniaturization of analytical systems
23. Highly sensitive time-resolved fluoroimmunoassay of human immunoglobulin E by using a new europium fluorescent chelate as a label
24. Synthesis of a new tetradentate beta-diketonate-europium chelate and its application for time-resolved fluorimetry of albumin
25. Highly sensitive quantitation of methamphetamine by time-resolved fluoroimmunoassay using a new europium chelate as a label
26. Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer
27. Homogeneous immunoassays using rare earth cryptates and time resolved fluorescence: Principles and specific advantages for tumor markers